Polypyrrole layers electrodeposited from TRIS buffer solution as matrix of tyrosinase biosensor

A novel supporting electrolyte – tris(hydroxymethyl)aminomethane hydrochloride (TRIS) buffer solution was used to obtain polypyrrole (PPy) layers based on the anodic oxidation of pyrrole. Influence of some parameters: temperature, monomer and supporting electrolyte concentration, polarization potential and hydrodynamic conditions on electrodeposition process were investigated. Obtained layers were preliminary tested in construction of an amperometric biosensor based on enzyme tyrosinase, which response was tested using catechol as a substrate

Meldola's Blue : doped titania sol-gel sensor for NADH determination

Titania layers obtained by a sol-gel technique doped with redox mediator, Meldola’s Blue, were employed for construction of a new NADH senor. Optimization of preparation process as well as experimental conditions affecting the response of the sensor were examined. Under optimal conditions NADH could be determined in the wide linear range from 90 to 2300 μM with detection limit 12 μM and a high sensitivity 12.5 nA μM-1. The usefulness of developed sensor was preliminarily checked in determination of NADH forming during enzymatic oxidation of ethanol catalyzed by alcohol dehydrogenase (ADH

Detection of NADH and ethanol at a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite film

For electrocatalytic determination of NADH, a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite was proposed. The composition of the matrix film was optimised in terms of the content of carbon nanotubes and Nafion. Incorporation of a redox mediator, Meldola’s Blue, into the nanocomposite film enabled electrocatalytic determination of NADH at a low potential, −50 mV. For determination of ethanol, alcohol dehydrogenase (ADH) was immobilized into the matrix layer. Experimental conditions affecting the biosensor response were examined, including enzyme loading, temperature of measurement and pH of background electrolyte. Assessments of the analytical characteristics of the biosensor were performed with respect to sensitivity, limit of detection, operational stability, repeatability and reproducibility. The proposed biosensor showed electrocatalytic activity toward oxidation of ethanol with sensitivity of 2.24 µA L mmol^{^{-1}}, linear range from 0.05 to 1.1 mmol L^{-1}, and limit of detection of 25 µmol L^{-1}. The apparent Michaelis-Menten constant was 1.24 mmol L^{-1}, indicating a high biological affinity of ADH/titania sol-gel/Meldola’s Blue/MWCNT/Nafion electrode for ethanol. The developed biosensor was tested in determinations of ethanol content in alcoholic beverages

Monitoring of Scour Around Bridge Piers and Abutments

AbstractScour of the riverbeds around bridge supports is the most frequent cause of their failures. Maintenance and repair costs of the bridges damaged by scour effects are significant, but it is estimated that the social costs are five times higher than the direct repair and replacement costs.The bridge supports are subjected to the scour effects due to the erosive action of the flowing water, involving soil loosening from the bottom and the banks of the watercourse. The condition for the proper monitoring of scour is to understand its nature. The knowledge of the phenomena occurring during the high water flow in the area of the bridge supports is crucial to properly assess the current condition and to develop proper maintenance actions.Scour may be the consequence of:•narrowing the watercourse – a natural or man-made, including construction of a bridge,•lateral movement or lowering of the stream bed,•hydraulic works shortening the length of the meandering section of the watercourse,•changes occurring in the catchment area of the watercourse,•other changes in watercourse hydrology.Construction of a bridge in the certain area disturbs natural stream flow conditions, especially the flood water and may change the terms of the normal water flow. The presence of a bridge causes the stream flow cross-section reduction, which increases the speed and intensity of erosion of the streambed. River tends to stabilize its bed in order to restore the natural flow section. Bridge supports also change the laminar water flow and turbulent flow.Scour present around a bridge supports can be monitored by the mobile and fixed devices.Portable scour monitoring devices are mainly: different types of probes such as: sticks, tape or rope with weights and bars used by divers and sonar acoustic measuring devices.Stationary equipment is used for continuous or regular scour monitoring of the bridge supports of the bridge, for example once a day, once a week. They are stationary devices including various types of robotic probes and stationary hydroacoustic measurement systems. Stationary device can be installed on a support or near the bridge, usually at the head of the pillar, or in the ground near the bottom of the watercourse. It should be installed near the site of the anticipated greatest scour. The device interacts with the data logger, which can be read on the site or transmitted to a remote control unit.The article presents the principles of scour monitoring near the bridge supports, developed during the project “Monitoring system for bridge supports and their surrounding areas” co-founded by the Polish National Centre for Research and Development under the program Innotech. During the project monitoring system for bridge supports was developed with specialized software for online data visualization. The article presents selected measurement results from the sonar measurements

The protective role of carbon monoxide (CO) produced by heme oxygenases and derived from the CO-releasing molecule CORM-2 in the pathogenesis of stress-induced gastric lesions : evidence for non-involvement of nitric oxide (NO)

Carbon monoxide (CO) produced by heme oxygenase (HO)-1 and HO-2 or released from the CO-donor, tricarbonyldichlororuthenium (II) dimer (CORM-2) causes vasodilation, with unknown efficacy against stress-induced gastric lesions. We studied whether pretreatment with CORM-2 (0.1–10 mg/kg oral gavage (i.g.)), RuCl3 (1 mg/kg i.g.), zinc protoporphyrin IX (ZnPP) (10 mg/kg intraperitoneally (i.p.)), hemin (1–10 mg/kg i.g.) and CORM-2 (1 mg/kg i.g.) combined with NG-nitro-l-arginine (l-NNA, 20 mg/kg i.p.), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 10 mg/kg i.p.), indomethacin (5 mg/kg i.p.), SC-560 (5 mg/kg i.g.), and celecoxib (10 mg/kg i.g.) affects gastric lesions following 3.5 h of water immersion and restraint stress (WRS). Gastric blood flow (GBF), the number of gastric lesions and gastric CO and nitric oxide (NO) contents, blood carboxyhemoglobin (COHb) level and the gastric expression of HO-1, HO-2, hypoxia inducible factor 1α (HIF-1α), tumor necrosis factor α (TNF-α), cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) were determined. CORM-2 (1 mg/kg i.g.) and hemin (10 mg/kg i.g.) significantly decreased WRS lesions while increasing GBF, however, RuCl3 was ineffective. The impact of CORM-2 was reversed by ZnPP, ODQ, indomethacin, SC-560 and celecoxib, but not by l-NNA. CORM-2 decreased NO and increased HO-1 expression and CO and COHb content, downregulated HIF-1α, as well as WRS-elevated COX-2 and iNOS mRNAs. Gastroprotection by CORM-2 and HO depends upon CO’s hyperemic and anti-inflammatory properties, but is independent of NO

Research on measurement of electromagnetic fields generated by electric and combustion powered rolling stock.

Axle counters are more and more often applied in train detection systems. The wheel sensor is a main part of each axle counter system. In parallel, more and more complex railway vehicles, especially traction ones, are a potential source of interferences influencing the operation of these train detection systems. It is the reason to verify the electromagnetic compatibility (EMC) between the signalling equipment, particularly train detection systems and new vehicles in the process of obtaining the permission for their exploitation. The measurement of interfering magnetic fields generated by vehicles is one of tests to be carried out. For the simplification and unification purpose of the applied interference test methods the EN 50238 standard and TS 50238-3 technical specification were developed. The specification defines unified testing procedures. However, it is necessary to verify if it may replace different testing methods used in particular European states. It is the goal of the European research project financed from the TEN-T network resources