BAL fluid cytology proportions for mice fed (n-3) or (n-6) PUFA enriched foods for 8 weeks and then sensitized and challenged with OVA for an additional 4 weeks compared with mice fed control food that were sensitized and challenged with saline (Negative Control) or OVA (Positive Control)¹.

<p>BAL fluid cytology proportions for mice fed (n-3) or (n-6) PUFA enriched foods for 8 weeks and then sensitized and challenged with OVA for an additional 4 weeks compared with mice fed control food that were sensitized and challenged with saline (Negative Control) or OVA (Positive Control)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163819#t004fn001" target="_blank">¹</a>.</p

Time line for the experimental protocol.

<p>Mice continued to eat their respective foods throughout the allergen sensitization and challenge period. They were sensitized with saline or OVA at 8 and 10 weeks, and then challenged by nebulization with saline or OVA once at 10 weeks and on three consecutive days during week 12. Mice were sacrificed 24 h after the final nebulization.</p

Plasma concentrations (g/100 g FA) of selected FA in mice after dietary intervention for 12 weeks¹.

<p>Plasma concentrations (g/100 g FA) of selected FA in mice after dietary intervention for 12 weeks<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163819#t003fn001" target="_blank">¹</a>.</p

Composition of selected FA in diets fed to mice for 12 weeks.

<p>Composition of selected FA in diets fed to mice for 12 weeks.</p

Percent of lung tissue staining positive with periodic acid-Schiff (PAS) stain for mucus and rat anti-mouse CD45 stain, after mice were fed (n-3) or (n-6) PUFA enriched foods for 8 weeks and then sensitized and challenged with OVA for an additional 4 weeks compared with mice fed control food that were sensitized and challenged with saline (Negative Control) or OVA (Positive Control)¹.

<p>Percent of lung tissue staining positive with periodic acid-Schiff (PAS) stain for mucus and rat anti-mouse CD45 stain, after mice were fed (n-3) or (n-6) PUFA enriched foods for 8 weeks and then sensitized and challenged with OVA for an additional 4 weeks compared with mice fed control food that were sensitized and challenged with saline (Negative Control) or OVA (Positive Control)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163819#t006fn001" target="_blank">¹</a>.</p

Representative light microscopy images of lung tissue.

<p>Lung tissues were stained with rat anti-mouse CD45 after mice were fed their respective foods for 12 weeks. They were sensitized and challenged with saline or OVA starting at 8 weeks. (A) Only circulating leukocytes are seen in control mice fed standard rodent food and sensitized and challenged with saline (Negative Control). (B) Mice fed standard rodent food and sensitized and challenged with OVA (Positive Control) or (C) Corn Oil-enriched food exhibit a marked increase in CD45+ inflammatory cells, particularly peribronchial infiltrates. (D) Overall, there were less CD45+ inflammatory cells in mice fed Fish-Oil enriched food compared with mice fed control food (Positive Control; <i>P</i> = 0.04). Original magnification 25X.</p

Cytokine (IL-4 and IL-13) concentrations in BAL fluid of mice fed (n-3) or (n-6) PUFA enriched foods for 8 weeks and then sensitized and challenged with OVA for an additional 4 weeks compared with mice fed control food that were sensitized and challenged with saline (Negative Control) or OVA (Positive Control)¹.

<p>Cytokine (IL-4 and IL-13) concentrations in BAL fluid of mice fed (n-3) or (n-6) PUFA enriched foods for 8 weeks and then sensitized and challenged with OVA for an additional 4 weeks compared with mice fed control food that were sensitized and challenged with saline (Negative Control) or OVA (Positive Control)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163819#t005fn001" target="_blank">¹</a>.</p

Average nutrient composition (dry matter basis) of diets fed to mice for 12 weeks.

<p>Average nutrient composition (dry matter basis) of diets fed to mice for 12 weeks.</p