Agreement between the Open Barbell and Tendo Linear Position Transducers for Monitoring Barbell Velocity during Resistance Exercise

To determine the agreement between the Open Barbell (OB) and Tendo weightlifting analyzer (TWA) for measuring barbell velocity, eleven men (19.4 ± 1.0 y) performed one set of 2–3 repetitions at four sub-maximal percentage loads, [i.e., 30, 50, 70, and 90% one-repetition maximum (1RM)] in the back (BS) and front squat (FS) exercises. During each repetition, peak and mean barbell velocity were recorded by OB and TWA devices, and the average of the 2–3 repetitions was used for analyses. Although the repeated measures analysis of variance revealed significantly (p ≤ 0.005) greater peak and mean velocity scores from OB across all intensities, high intraclass correlation coefficients (ICC2,K = 0.790–0.998), low standard error of measurement (SEM2,K = 0.040–0.119 m·s−1), and coefficients of variation (CV = 2–4%) suggested consistency between devices. Positive (r = 0.491–0.949) Pearson correlations between averages and differences (between devices) in peak velocity, as well as associated Bland-Altman plots, showed greater differences occurred as the velocity increased, particularly at low-moderate intensity loads. OB consistently provides greater barbell velocity scores compared to TWA, and the differences between devices were more apparent as the peak velocity increased with low-to-moderate loads. Strength coaches and athletes may find better agreement between devices if the mean velocity scores are only considered

Mediators of Monocyte Migration in Response to Recovery Modalities following Resistance Exercise

Mediators of monocyte migration, complement receptor-3 (CR3), and chemokine ligand-4 (CCL4) were measured in response to recovery modalities following resistance exercise. Thirty resistance-trained men (23.1 +/- 2.9 y; 175.2 +/- 7.1 cm; 82.1 +/- 8.4 kg) were given neuromuscular electric stimulation (NMES), cold water immersion (CWI), or control (CON) treatments immediately following resistance exercise. Blood samples were obtained preexercise (PRE), immediately (IP), 30 minutes (30 P), 24 hours (24 H), and 48 hours (48 H) after exercise for measurement of circulating CCL4 and CR3 expression on CD14+ monocytes, by assay and flow cytometry. Circulating CCL4 showed no consistent changes. Inferential analysis indicated that CR3 expression was likely greater in CON at 30 P than NMES (90.0%) or CWI (86.8%). NMES was likely lower than CON at 24H (92.9%) and very likely lower at 48H (98.7%). Expression of CR3 following CWI was very likely greater than CON (96.5%) at 24H. The proportion of CR3+ monocytes was likely greater following CWI than NMES (85.8%) or CON (85.2%) at 24 H. The change in proportion of CR3+ monocytes was likely (86.4%) greater following NMES than CON from IP to 30 P. The increased expression of CR3 and increased proportion of CR3+ monocytes following CWI at 24 H indicate a potentially improved ability for monocyte adhesion to the endothelium, possibly improving phagocytosis of damaged tissues

Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in Escherichia coli

Unraveling the mechanisms of microbial adaptive evolution following genetic or environmental challenges is of fundamental interest in biological science and engineering. When the challenge is the loss of a metabolic enzyme, adaptive responses can also shed significant insight into metabolic robustness, regulation, and areas of kinetic limitation. In this study, whole-genome sequencing and highresolution C-13-metabolic flux analysis were performed on 10 adaptively evolved pgi knockouts of Escherichia coli. Pgi catalyzes the first reaction in glycolysis, and its loss results in major physiological and carbon catabolism pathway changes, including an 80% reduction in growth rate. Following adaptive laboratory evolution (ALE), the knockouts increase their growth rate by up to 3.6-fold. Through combined genomic-fluxomic analysis, we characterized the mutations and resulting metabolic fluxes that enabled this fitness recovery. Large increases in pyridine cofactor transhydrogenase flux, correcting imbalanced production of NADPH and NADH, were enabled by direct mutations to the transhydrogenase genes sthA and pntAB. The phosphotransferase system component crr was also found to be frequently mutated, which corresponded to elevated flux from pyruvate to phosphoenolpyruvate. The overall energy metabolism was found to be strikingly robust, and what have been previously described as latently activated Entner-Doudoroff and glyoxylate shunt pathways are shown here to represent no real increases in absolute flux relative to the wild type. These results indicate that the dominant mechanism of adaptation was to relieve the rate-limiting steps in cofactor metabolism and substrate uptake and to modulate global transcriptional regulation from stress response to catabolism

Obesity-Induced Colorectal Cancer Is Driven by Caloric Silencing of the Guanylin-GUCY2C Paracrine Signaling Axis.

Obesity is a well-known risk factor for colorectal cancer but precisely how it influences risks of malignancy remains unclear. During colon cancer development in humans or animals, attenuation of the colonic cell surface receptor guanylyl cyclase C (GUCY2C) that occurs due to loss of its paracrine hormone ligand guanylin contributes universally to malignant progression. In this study, we explored a link between obesity and GUCY2C silencing in colorectal cancer. Using genetically engineered mice on different diets, we found that diet-induced obesity caused a loss of guanylin expression in the colon with subsequent GUCY2C silencing, epithelial dysfunction, and tumorigenesis. Mechanistic investigations revealed that obesity reversibly silenced guanylin expression through calorie-dependent induction of endoplasmic reticulum stress and the unfolded protein response in intestinal epithelial cells. In transgenic mice, enforcing specific expression of guanylin in intestinal epithelial cells restored GUCY2C signaling, eliminating intestinal tumors associated with a high calorie diet. Our findings show how caloric suppression of the guanylin-GUCY2C signaling axis links obesity to negation of a universal tumor suppressor pathway in colorectal cancer, suggesting an opportunity to prevent colorectal cancer in obese patients through hormone replacement with the FDA-approved oral GUCY2C ligand linaclotide

IDCS J1426.5+3508: Cosmological implications of a massive, strong lensing cluster at Z = 1.75

The galaxy cluster IDCS J1426.5+3508 at z = 1.75 is the most massive galaxy cluster yet discovered at z > 1.4 and the first cluster at this epoch for which the Sunyaev-Zel'Dovich effect has been observed. In this paper we report on the discovery with HST imaging of a giant arc associated with this cluster. The curvature of the arc suggests that the lensing mass is nearly coincident with the brightest cluster galaxy, and the color is consistent with the arc being a star-forming galaxy. We compare the constraint on M200 based upon strong lensing with Sunyaev-Zel'Dovich results, finding that the two are consistent if the redshift of the arc is z > 3. Finally, we explore the cosmological implications of this system, considering the likelihood of the existence of a strongly lensing galaxy cluster at this epoch in an LCDM universe. While the existence of the cluster itself can potentially be accomodated if one considers the entire volume covered at this redshift by all current high-redshift cluster surveys, the existence of this strongly lensed galaxy greatly exacerbates the long-standing giant arc problem. For standard LCDM structure formation and observed background field galaxy counts this lens system should not exist. Specifically, there should be no giant arcs in the entire sky as bright in F814W as the observed arc for clusters at z \geq 1.75, and only \sim 0.3 as bright in F160W as the observed arc. If we relax the redshift constraint to consider all clusters at z \geq 1.5, the expected number of giant arcs rises to \sim15 in F160W, but the number of giant arcs of this brightness in F814W remains zero. These arc statistic results are independent of the mass of IDCS J1426.5+3508. We consider possible explanations for this discrepancy.Comment: 7 pages, 4 figures, Accepted to The Astrophysical Journa

Effects of beta-Hydroxy-beta-methylbutyrate Free Acid Ingestion and Resistance Exercise on the Acute Endocrine Response

Objective. To examine the endocrine response to a bout of heavy resistance exercise following acute beta-hydroxy-beta-methylbutyrate free acid (HMB-FA) ingestion. Design. Twenty resistance trained men were randomized and consumed either 1 g of HMB-FA (BetaTor) or placebo (PL) 30 min prior to performing an acute heavy resistance exercise protocol. Blood was obtained before (PRE), immediately after (IP), and 30 min after exercise (30P). Circulating concentrations of testosterone, growth hormone (GH), insulin-like growth factor (IGF-1), and insulin were assayed. Data were analyzed with a repeated measures ANOVA and area under the curve (AUC) was analyzed by the trapezoidal rule. Results. The resistance exercise protocol resulted in significant elevations from PRE in testosterone (P \u3c 0.01), GH (P \u3c 0.01), and insulin (P = 0.05) at IP, with GH (P \u3c 0.01) and insulin (P \u3c 0.01) remaining elevated at 30P. A significant interaction was noted between groups in the plasma GH response at IP, which was significantly higher following HMB-FA compared to PL (P \u3c 0.01). AUC analysis revealed an elevated GH and IGF-1 response in the HMB-FA group compared to PL. Conclusion. HMB-FA prior to resistance exercise augments the GH response to high volume resistance exercise compared to PL. These findings provide further support for the potential anabolic benefits associated with HMB supplementation

Drivers of Bird- Window Collisions in Florida International University’s Modesto A. Maidique Campus

Bird-window collisions are among the top leading causes of death for birds in North America. Studies have found primary drivers of bird-window collisions to be window area, building size, and presence of vegetation around the buildings. We hypothesized that higher window area and vegetation would increase birdwindow collisions, with an inverse relationship between number of stories and bird-window collisions. Surveys were conducted for 21 consecutive days around six buildings at Florida International University (FIU) in October 2014. Using ArcGIS, 50m buffers were created around each building to calculate percent vegetation and ImageJ was used to calculate percent window cover. We collected 10 bird carcasses and 13 feather piles assumed to represent bird-window collisions. Most of the collisions (8 out of 21) occurred at the building with the lowest percent vegetation and low window area. Our sample size limits our ability to make general conclusions, however, our data is a part of a larger collaborative project that includes over 30 campuses across North America. Results from this larger project could provide insight on whether the magnitude of bird-window collisions relates to landscape structure and functional connectivity

A Uniform Analysis of the Ly-alpha forest at z = 0 - 5: II. Measuring the mean intensity of the extragalactic ionizing background using the proximity effect

A homogeneous sample of 99 moderate resolution QSO spectra at z > 1.7 were presented in Paper I, including 39 previously unpublished spectra from the Multiple Mirror Telescope. The statistics of the Lyman alpha forest were discussed. In this analysis, we demonstrate that a proximity effect is present in the data, ie. there exists a significant (5.5$\sigma$) deficit of lines at $z_{abs} \approx z_{em}$. Within 1.5 $h^{-1}$ Mpc of the QSO emission redshift, the significance does depend on QSO luminosity, in accordance with the theory that this effect is caused by enhanced ionization of hydrogen in the vicinity of the QSO from UV photons from the QSO itself. The photoionization model of Bajtlik, Duncan, and Ostriker (1988) permits an estimate of the mean intensity of the extragalactic background radiation at the Lyman limit. We compare the results of this standard analysis with those obtained using a maximum likelihood technique. The best fit value for $J(\nu_{0})$ is 7.0$^{+3.4}_{-4.4}$ x 10$^{-22}$ ergs/s/cm$^{2}$/Hz/sr, over the redshift range 1.7 < z < 3.8, using QSO redshifts based on narrow emission lines. The best fit value for the HI ionization rate is 1.9$^{+1.2}_{-1.0}$ x 10$^{-12}$ s$^{-1}$, in good agreement with models of the background which incorporate QSOs only. This large absorption line sample and these techniques for measuring the background and understanding the systematics involved allow us to place what we believe are are the firmest limits on the background at these redshifts.Comment: revised figures 13 and 14, and other minor corrections, 42 Latex pages, 23 encapsulated Postscript figures, uses emulateapj.sty, To appear in the Sept. 2000 ApJ