AutomatedDevelopmentof a KineticMethodfor the Continuous- Flow Determinationof Creatinine

A “stopped-flow ” method for the kinetic Jaff#{233}determi-nation of creatinine was developed, with the use of a computer-controlled continuous-flow system. Simplex optimization was used to find conditions of hydroxide and picrate giving maximum sensitivity for creatinine. We used a modified central composite experimental design to evaluate creatinine sensitivity andalbumin, glucose, and acetone interferences as functions of hydroxideand pic-rate concentrations.More importantly,this work illustrates that the automated development of clinical chemical methodsoffers an efficient meansof obtainingoptimized, well-understood analytical procedures for subsequent routine use in the clinical chemistry laboratory. AdditonalKeyphrasee: simplex optimization variation, sources of analytical error response-surface mapp