The Johnsonian Septemeber 29, 1969

The Johnsonian is the weekly student newspaper of Winthrop University. It is published during fall and spring semesters with the exception of university holidays and exam periods. We have proudly served the Winthrop and Rock Hill community since 1923.https://digitalcommons.winthrop.edu/thejohnsonian1960s/1202/thumbnail.jp

Protein and cell patterning in closed polymer channels by photoimmobilizing proteins on photografted poly(ethylene glycol) diacrylate

Definable surface chemistry is essential for many applications of microfluidic polymer systems. However, small cross-section channels with a high surface to volume ratio enhance passive adsorption of molecules that depletes active molecules in solution and contaminates the channel surface. Here, we present a one-step photochemical process to coat the inner surfaces of closed microfluidic channels with a nanometer thick layer of poly(ethylene glycol) (PEG), well known to strongly reduce non-specific adsorption, using only commercially available reagents in an aqueous environment. The coating consists of PEG diacrylate (PEGDA) covalently grafted to polymer surfaces via UV light activation of the water soluble photoinitiator benzoyl benzylamine, a benzophenone derivative. The PEGDA coating was shown to efficiently limit the adsorption of antibodies and other proteins to <5% of the adsorbed amount on uncoated polymer surfaces. The coating could also efficiently suppress the adhesion of mammalian cells as demonstrated using the HT-29 cancer cell line. In a subsequent equivalent process step, protein in aqueous solution could be anchored onto the PEGDA coating in spatially defined patterns with a resolution of <15 μm using an inverted microscope as a projection lithography system. Surface patterns of the cell binding protein fibronectin were photochemically defined inside a closed microfluidic device that was initially homogeneously coated by PEGDA. The resulting fibronectin patterns were shown to greatly improve cell adhesion compared to unexposed areas. This method opens for easy surface modification of closed microfluidic systems through combining a low protein binding PEG-based coating with spatially defined protein patterns of interest

Comparative Analysis of Phytochemical and Proximate Contents of Three Orphan Legume Species

Background and Objective: Orphan legume species can provide cheap and quality nutrients, serve as sources of herbal medicine, forage crop, biomass feedstock and green manure but largely unexploited. The present study investigated phytochemical and proximate contents of three orphan legumes comprising three accessions of African Yam Bean (AYB), four of Kersting’s Groundnut (KG) and six of Winged Beans (WB). Materials and Methods: The seeds were collected from the Genebank of the International Institute of Tropical Agriculture via the Standard Material Transfer Agreement (SMTA). The samples were oven-dried, grounded into a fine powder and analyzed for their biochemical profiles using standard procedures. Results: Carbohydrate, betacyanin, quinone and terpenes were found in the samples, while saponins were found only in AYB and KG accessions. Alkaloids and coumarin were only detected in samples of WB and KG. The crude protein was quite high among all the samples ranged from 18.4% in TSs-33 of AYB-52.7% in TPt-126 of WB with a mean of 30.16±3.01. Conclusion: The study indicates that the species and the accessions are rich in proteins and essential proximate contents that can be explored as sources of food and feeds for both humans and animals

BINARY SOFT PRE-SEPARATION AXIOMS IN BINARY SOFT TOPOLOGICAL SPACES

In this article, we introduce binary soft pre-separation axioms in binary soft Topological space along with several properties of binary soft pre τ△i , i = 0; 1; 2, binary soft pre-regular, binary soft pre τ△3 , binary soft pre-normal and binary soft τ△4 axioms using binary soft points. We also mention some binary soft invariance properties namely binary soft topological property and binary soft hereditary property. We hope that these results will be useful for the future study on binary soft topology to carry out general background for the practical applications and to solve the thorny problems containing doubts in different grounds

APPLICATION OF SOFT P-OPEN SET TO BINARY SOFT STRUCTURES

The main aim of this paper is to introduce a single structure which carries the subsets of X as well as the subsets of Y under the parameter E for studying the information about the ordered pair of soft subsets of X and Y. Such a structure is called a binary soft structure from X to Y. The purpose of this paper is to introduce certain binary soft weak axioms that are analogous to the axioms of topology

Role of Click Chemistry in Organic Synthesis

Click chemistry involves highly efficient organic reactions of two or more highly functionalized chemical entities under eco-benign conditions for the synthesis of different heterocycles. Several organic reactions such as nucleophilic ring-opening reactions, cyclo-additions, nucleophilic addition reactions, thiol-ene reactions, Diels Alder reactions, etc. are included in click reactions. These reactions have very important features i.e. high functional group tolerance, formation of a single product, high atom economy, high yielding, no need for column purification, etc. It also possesses several applications in drug discovery, supramolecular chemistry, material science, nanotechnology, etc. Being highly significant and valuable, we have elaborated on several aspects of click reactions in organic synthesis in this chapter. Recent advancements in the field of organic synthesis using click chemistry approach have been deliberated by citing last five years articles

Tumour-infiltrated cortex participates in large-scale cognitive circuits.

The extent to which tumour-infiltrated brain tissue contributes to cognitive function remains unclear. We tested the hypothesis that cortical tissue infiltrated by diffuse gliomas participates in large-scale cognitive circuits using a unique combination of intracranial electrocorticography (ECoG) and resting-state functional magnetic resonance (fMRI) imaging in four patients. We also assessed the relationship between functional connectivity with tumour-infiltrated tissue and long-term cognitive outcomes in a larger, overlapping cohort of 17 patients. We observed significant task-related high gamma (70-250 Hz) power modulations in tumour-infiltrated cortex in response to increased cognitive effort (i.e., switch counting compared to simple counting), implying preserved functionality of neoplastic tissue for complex tasks probing executive function. We found that tumour locations corresponding to task-responsive electrodes exhibited functional connectivity patterns that significantly co-localised with canonical brain networks implicated in executive function. Specifically, we discovered that tumour-infiltrated cortex with larger task-related high gamma power modulations tended to be more functionally connected to the dorsal attention network (DAN). Finally, we demonstrated that tumour-DAN connectivity is evident across a larger cohort of patients with gliomas and that it relates to long-term postsurgical outcomes in goal-directed attention. Overall, this study contributes convergent fMRI-ECoG evidence that tumour-infiltrated cortex participates in large-scale neurocognitive circuits that support executive function in health. These findings underscore the potential clinical utility of mapping large-scale connectivity of tumour-infiltrated tissue in the care of patients with diffuse gliomas

Recent Advances in the Pathobiology of Hodgkin's Lymphoma: Potential Impact on Diagnostic, Predictive, and Therapeutic Strategies

From its first description by Thomas Hodgkin in 1832, Hodgkin's disease, now called Hodgkin's lymphoma, has continued to be a fascinating neoplasm even to this day. In this review, historical aspects, epidemiology, diagnosis, tumor biology, new observations related to host-microenvironment interactions, gene copy number variation, and gene expression profiling in this complex neoplasm are described, with an exploration of chemoresistance mechanisms and potential novel therapies for refractory disease

The Impact of Organizational Culture on Performance foundation of Kindergartens Managers in Lattakia governorate - Afield Study

This Research aimed to Recognize the reality of the prevailing organizational culture on performance foundation of kindergartens managers in lattakia governorate, and recognize impact of organizational culture on performance foundation of kindergartens managers in lattakia governorate, also the impact of the variables ( the training courses, years of experience, the scientific qualification) in organizational culture, and Performance foundation. To achieve the object of the research a questionnaire has been included two dimensions to measures the Organizational Culture and Performance foundation then applied to a sample of (82) Kindergarten Managers for the academic year 2018/2019. And used the Descriptive analytical Method. Validity of the questionnaire was established though a jury of (7) of the teaching staff of educational at Damascus and Tishreen Universities. Pilot sample consisted of (26) Kindergartens Managers, Reliability was established by Cronbach – Alpha Reliability (0.972) for Organizational Culture dimensions, and (0.961) for Performance foundation to Kindergartens Managers. The research ended to the Degree of Organizational Culture Dimensions of Kindergartens Managers in Lattakia governorate was middle degree, and the Reality of the Prevailing Organizational Culture on Performance foundation of Kindergartens Managers in Lattakia governorate was middle degree. The Results have  Shown  a good Correlation connection Between Organizational Culture  and Performance foundation for Kindergartens Managers, also there are found  Impact of the variables (the training courses, years of experience, the scientific qualification) in Organizational Culture, and haven’t  Shown  the Impact of these variables in Performance foundation of Kindergartens Managers in Lattakia governorate.