Ann Trop Med Parasitol.

The isoenzyme profiles of a group of five Trypanosoma cruzi stocks obtained from congenital cases and of a second group of seven stocks obtained from chagasic mothers who did not transmit their infection congenitally were studied by electrophoresis of nine enzymes. All the mothers became infected in Bahia State (Brazil). With the exception of a triple 'heterozygous' GPI pattern, all T. cruzi stocks were identified as zymodeme 2 (Z2). The heterozygous pattern has not been recorded previously in Bahia. This study shows that enzymically similar T. cruzi stocks can show different behaviour with respect to transplacental transmission, and also with respect to clinicopathological presentation and therapeutic response of the congenital cases

Evaluation of the histopathological classifications of american cutaneous and mucocutaneous leishmaniasis

Barral, Aldina Maria Prado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-07-14T19:01:43Z No. of bitstreams: 1 Bittencourt ACL Evaluation of the histopathological....pdf: 344770 bytes, checksum: 0f5b694f66409e1e9c450e065c6538e8 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-07-14T19:18:59Z (GMT) No. of bitstreams: 1 Bittencourt ACL Evaluation of the histopathological....pdf: 344770 bytes, checksum: 0f5b694f66409e1e9c450e065c6538e8 (MD5)Made available in DSpace on 2017-07-14T19:18:59Z (GMT). No. of bitstreams: 1 Bittencourt ACL Evaluation of the histopathological....pdf: 344770 bytes, checksum: 0f5b694f66409e1e9c450e065c6538e8 (MD5) Previous issue date: 1991NIH grant AI 16282-11 and FINEPUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Laboratórios de Anatomia Patológica e Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Laboratórios de Anatomia Patológica e Imunologia. Salvador, BA, BrasilIn order to evaluate the reliability of histopathological classifications of cutaneous and mucocutaneous leishmaniasis the authors compared the histopathological patterns of two biopsies taken simultaneously from the same patient, and classified the material according to Ridley et al. (1980), to Magalhães et al (1986a), and to a more simplified classification with only three patterns. Distinct histopathological aspects were observed in diferente lesions or even in the same lesion. The authors concluded that histopathological patterns do not represent a stage of tegumentar leishmaniasis, thus they can not be correlated with prognosis and therapeutical response as suggested in the literature

Adult T-cell leukemia/lymphoma

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2015-06-11T14:21:04Z No. of bitstreams: 1 Bittencourt, AL. Leucemis linfoma de células T....pdf: 5046133 bytes, checksum: 8a1fb41a0749f476753dfee90473c995 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2015-06-11T14:35:17Z (GMT) No. of bitstreams: 1 Bittencourt, AL. Leucemis linfoma de células T....pdf: 5046133 bytes, checksum: 8a1fb41a0749f476753dfee90473c995 (MD5)Made available in DSpace on 2015-06-11T14:35:17Z (GMT). No. of bitstreams: 1 Bittencourt, AL. Leucemis linfoma de células T....pdf: 5046133 bytes, checksum: 8a1fb41a0749f476753dfee90473c995 (MD5) Previous issue date: 2008Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilResumo: A leucemia/linfoma de células T do adulto (ATL) é tipo agressivo de doença linfoproliferativa causada pelo vírus linfotrópico para células T humanas (HTLV-I), geralmente fatal e que não responde a quimioterapia. Classifica-se em formas aguda, crônica, linfomatosa e indolente (smoldering). Outra forma clínica, a tumoral primária de pele, com características diferentes, foi sugerida recentemente. As formas aguda, linfomatosa e tumoral primária de pele são as de pior prognóstico. Os critérios diagnósticos de ATL são: sorologia positiva para o HTLV-I; diagnóstico citológico ou histológico de leucemia/linfoma de células T, CD4+/CD25+; presença de linfócitos T anormais em sangue periférico; confirmação de integração monoclonal do DNA proviral do HTLV-I. Há lesões de pele em cerca de 70% dos casos, que podem ser primários (formas indolente e tumoral primária da pele) ou secundários. As lesões cutâneas são múltiplas, sendo as mais freqüentes a eritrodermia, as pápulas e as placas. A ATL não tem aspecto histológico característico, podendo apresentar padrões superponíveis ao linfoma periférico T não especificado, à micose fungóide ou ao linfoma anaplásico de grandes células. O padrão imuno-histoquímico pode também simular o de outros tipos de linfoma T. Por esse motivo, é muito importante que no Brasil seja solicitada sorologia para o HTLV-I em todos os casos de leucemia e/ou linfoma de células T maduras.Abstract: Adult T cell leukemia/lymphoma (ATL) is an aggressive type of lymphoproliferative disease associated with the human T-cell lymphotropic virus type I (HTLV-I) that is characterized by a short survival time and absence of response to chemotherapy. ATL is classified into four clinical types: acute, chronic, lymphoma, and smoldering. Another clinical form of ATL, the primary cutaneous tumoral, with diverse characteristics, has been recently suggested. Patients with acute, lymphoma and primary cutaneous tumoral types have a poor prognosis. The diagnostic criteria of ATL consist of: positive serology for HTLV-I; cytologic or histologic confirmation of CD4+/CD25+ T-cell leukemia/lymphoma; abnormal T lymphocytes in peripheral blood; and confirmation of monoclonal integration of HTLV-I proviral DNA. There is skin involvement in around 70% of ATL cases, which could be primary (smoldering and primary cutaneous tumoral) or secondary. The skin lesions are multiple, erythroderma, papules and plaques being the most common. ATL has no characteristic histological pattern, and may present patterns that could superimpose nonspecific peripheral T-cell lymphoma, mycosis fungoides or anaplastic large cell lymphoma. The immunohistochemistry pattern may also be similar to that of other T-cell lymphomas. Thus, it is very important that in Brazil HTLV-I infection be investigated in all mature T-cell leukemias/lymphoma

Microsatellite alterations are also present in the less aggressive types of adult T-cell leukemia-lymphoma

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2016-04-12T17:41:31Z No. of bitstreams: 1 Magalhaes, M. Microsatellite alterations....pdf: 320318 bytes, checksum: f7780b37b7d568b38ab42107147e4d61 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2016-04-12T17:51:50Z (GMT) No. of bitstreams: 1 Magalhaes, M. Microsatellite alterations....pdf: 320318 bytes, checksum: f7780b37b7d568b38ab42107147e4d61 (MD5)Made available in DSpace on 2016-04-12T17:51:50Z (GMT). No. of bitstreams: 1 Magalhaes, M. Microsatellite alterations....pdf: 320318 bytes, checksum: f7780b37b7d568b38ab42107147e4d61 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Departamento de Dermatologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Departamento de Patologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil / National Institute of Science and Technology of Tropical Diseases. INCT. Salvador, BA, BrasilBACKGROUND: Adult T-cell leukemia/lymphoma (ATL) is a mature T-cell neoplasia etiologically linked to HTLV-1. Manifestations of ATL are diverse and different clinical types with different tissue involvement and aggressiveness have been described. The mechanisms that lead to the development of ATL clinical types have not yet been clarified. Considering that in ATL patients HTLV-1 infection generally occurs in childhood, a multistep carcinogenesis model has been proposed. Microsatellite alterations are important genetic events in cancer development and these alterations have been reported in the aggressive types of ATL. Little is known about oncogenesis of the less aggressive types. METHODOLOGY/PRINCIPAL FINDINGS: In this study we investigated the role of the microsatellite alterations in the pathogenesis mediated by HTLV-1 in the different types of ATL. We examined the presence of microsatellite instability (MSI) and loss of heterozigosity (LOH) in matched pair samples (tumoral and normal) of 24 patients with less aggressive types (smoldering and chronic) and in aggressive types (acute and lymphoma) of ATL. Four microsatellite markers D10S190, D10S191, D1391 and DCC were analyzed. MSI was found in four patients, three smoldering and one chronic, and LOH in four patients, three smoldering and one acute. None of the smoldering patients with microsatellite alterations progressed to aggressive ATL. CONCLUSIONS/SIGNIFICANCE: To our knowledge, this is the first report describing the presence of MSI and LOH in the less aggressive types of ATL. These results indicate that microsatellite alterations may participate in the development of the less aggressive types of AT