Prevalence of parasitical infestations in sheep from the Didactic Station of the University of Agricultural Sciences and Veterinary Medicine of Iaşi, during 2003-2005

The parasitical cases were monitored in sheep from the Experimental Didactic Station of the University of Agricultural Sciences and Veterinary Medicine of Iaşi, during 2003-2005. Cestode (Thysaniezia giardi), trematodes (Dicrocoelium lanceatum), nematode (Trichostrongyloidea/Trichostrongylidae family, Dictyocaulida family; Metastrongyloidea/Protostrongylidae family) and arthropods from Arachnida classes (Acari subclass) and Insecta caused the parasitoses diagnosed in that period. Every year, psoroptic scabies has affected sheep during stable period, when the lowest temperatures of the season were reached. Grazing after the prophylactic autumn disinfection developed a trichostrongili population in the body of hosts, which was sufficient to pollute grassland and perpetuate parasitosis. The interruption of biological cycle by adult or one of the intermediary host systematic destroying has resulted in the diminution of dicroceliosis incidenc

Farmed cyprinids diseases from the Prut River Basin

The current paper aims to outline the most common fish diseases that affect farmed cyprinids in ponds from the Prut river basin, in order to better understand and reduce fish health risks. After 2 field work expeditions to Rompescaris farm -Podu-Iloaiei from Iasi county and to Dracșani fish farm from Piscicola-Botoșani county, biological samples were collected, in May 2021. Using net fishing the following fish species were harvested: carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix) and mirror carp (Cyprinus carpio var. specularis). the biological material, the moribund specimens with visible lesions were isolated and the apparently healthy specimens were released. The collected specimens were subjected to a clinical, a parasitological and a bacteriological investigation

The prevalence of giardiosis in animals and humans in Iași county with the establishment of zoonotic risk

The study on the prevalence of Giardia sp. in bovines was performed by analysing the cases recorded at Dancu Research Station, the results showing that this is a protozoan, commonly found in bovine farms, being one of the main causes of diarrhoea in calves. The source of contamination with Giardia sp. is represented by drinking water, breast milk and environment. In the period 2017-2020, the cases of giardiosis diagnosed in pets at the Faculty of Veterinary Medicine were analysed; a coinfection was found in dogs in a proportion of 64% with yeast cells, 22% with Cryptosporidium sp., 6% with Isospora sp. and only in 3% of cases Giardia sp. has been reported as the only pathogen involved in the clinical picture. The study on the prevalence of Giardia sp. in humans during 2017-2020 was performed using data provided by the Praxis Laboratory. The conclusions demonstrate the presence of a high rate of giardiosis in pets (dogs, cats), in farm animals (bovine), and in humans, each representing a source of contamination of the environment and of the other categories. The results showed for both humans and animals that drinking water can be a major source of infection with Giardia sp., requiring the much more frequent and rigorous control of drinking water

Observations regarding in vitro hatching of Raillietina spp. (Cestoda: Cyclophyllidea) onchosphere

The study has been realised in May 2016 having as purpose the observation of the onchosphere hatching process. The eggs of Raillietina spp. were aquired from feces belonging to Great Spotted Woodpecker (Dendrocopos major) that was naturaly infested. Initally, the samples were examined from a parasitological point of view using the Willis method. The egg concentrate has been obtained using the protocol described by VOGE and all. (1961) with some adjustments. The egg suspension was incubated at 30 oC for an hour. The hatching process of the oncosphere has been observed on the slide with the optical microscope (Motic B series) fitted with a Moticam 1000 camera, using the x400 magnification. Measuring has been realised with Motic Images Plus 2.0 software. The temperature of the slide in the moment of examination was 32oC. The whole hatching proces lasted 5 hours and went through the following stages: after half an hour the hexachant embryo has broken the basal membrane and entered the vitelline layer, the onchosphere was vigorously pushing and scraping the granular structure belonging to the vitelline layer, then the vitelline membrane and the egg shell was perforated after four and a half hours, thus completing the actual hatching process. After hatching, the onchosphere engaged in a series of swim-like motions in the liquid mass

First report of Dirofilaria repens infection in a microfilaraemic cat from Romania

The present study describes the first report of Dirofilaria repens infection with the presence of both microfilariae and adult nematodes in a cat from Northeastern Romania. Briefly, a 5-year-old male mixed breed cat was presented to a veterinary clinic in Iasi (Romania), for neutering, in early February 2020. During the surgery, two whitish worms were removed from the internal part of the scrotum. Two adult nematodes, one female and one male, were identified, on the basis of morphological features, as D. repens with whitish, cylindrical bodies, measuring 12.5 cm and 6.5 cm in length, respectively. At histology, the female nematode showed two cavitated structures containing myriads of variably arranged microfilariae. The male had a transversal diameter of 350 μm, a 10 μm thick cuticle and a ridge-period of 10 Мm. multiplex PCR confirmed the diagnosis of D. repens from both nematodes. The Knott’s test revealed the presence of microfilariae of D. repens. Routine biochemistry panel was within range with one exception, urea serum level slightly increased. The haematology results revealed an increased number of neutrophils, lymphocytes and eosinophils. The cat had an infection with Otodectes cynotis as well. The cat was discharged with the following therapy recommended: oral doxycycline (10 mg/kg) for 30 days and topical moxidectin, monthly doses. After six months, the Knott’s test gave negative results. Further studies should include new insights of D. repens infection in cats concerning its epidemiology, diagnosis and control

Epidemiology and clinical presentation of dogs infected with sarcoptic mange

Sarcoptic mange in dog is a common parasitic dermatitis, especially in non-controlled, stray dogs that develop evocative clinical signs. The present study includes 48 dogs, with different backgrounds, both privately owned and dogs from shelters. We searched for predisposing factors for contacting sarcoptic mange, such as: age, sex, breed, source of contamination. Their age ranged from 1.15 months to 12 years (with more than a half being under 1 year old), they were mostly common or cross-breed dogs, and the sex ratio was almost equal, with 25 females and 23 males. All dogs were naturally infected with Sarcoptes scabiei, as confirmed by identification on the microscope of skin scrapings from different body areas. Furthermore, we aimed to have a general idea concerning the severity of infection with Sarcoptes scabiei in the dogs we studied. The dogs received a clinical score based on the evaluation of typical signs that appear in sarcoptic mange such as alopecia, erythema, scales/crusts and the extent of mange on the cutaneous surface on different body parts (head, trunk, legs, tail). The most affected body part was the head, followed by the trunk and the most scored sign was the extent of affected skin and alopecia. The treatment consisted in the administration of either afoxolaner (Nexgard®), twice at a monthly interval (2.7-6.9 mg/kg), either sarolaner (Simparica®), twice at a monthly interval (2-4 mg/kg), or doramectin (Dectomax®) 0.2 mg/kg, twice at 14 days interval

Septicemic Outbreak in A Rainbow Trout Intensive Aquaculture System: Clinical Finds, Etiological Agents, and Predisposing Factors

On the 23rd of September 2022, a small intensive aquaculture unit populated with rainbow trout (Oncorhynchus mykiss) reported increased mortality in adults and juvenile fish. The unit comprised 12 enclosed concrete basins with a capacity of ten cubic meters of water, populated with 150 kg of fish each. Fish were subjected to a clinical examination on the site, after which whole fish were harvested for a bacteriological and histopathological examination. Water quality parameters were examined using classic biochemical methods and Fourier Transform Infrared Spectroscopy in order to find out whether the environment in which the fish live is also a predisposing factor that could facilitate different pathogens and induce a state of disease in the fish. Real-time PCR was performed on strains of Aeromonas spp. sampled from the fish to accurately identify the pathogen species. The goal was to accurately identify the problems and predisposing factors that lead to disease outbreaks

Fifth European Dirofilaria and Angiostrongylus Days (FiEDAD) 2016

Peer reviewe

Climate Change Is Increasing the Risk of the Reemergence of Malaria in Romania

The climatic modifications lead to global warming; favouring the risk of the appearance and development of diseases are considered until now tropical diseases. Another important factor is the workers’ immigration, the economic crisis favouring the passive transmission of new species of culicidae from different areas. Malaria is the disease with the widest distribution in the globe. Millions of people are infected every year in Africa, India, South-East Asia, Middle East, and Central and South America, with more than 41% of the global population under the risk of infestation with malaria. The increase of the number of local cases reported in 2007–2011 indicates that the conditions can favour the high local transmission in the affected areas. In the situation presented, the establishment of the level of risk concerning the reemergence of malaria in Romania becomes a priority