Process for the production of Pen V acylase from Streptomyces Lavendula

Penicillin VThe invention relates to a method of producing the penicillin V acylase enzyme from Streptomyces lavendulae in recombinant micro-organisms. The inventive enzyme production method comprises the following steps: (1) identification of the DNA corresponding to the pva gene, which encodes the penicillin V acylase enzyme from S. lavendulae ATCC 13664, (2) cloning of said gene in a vector suited to the host cell used, (3) cultivation of recombinant host cells bearing the pva gene, and (4) recovery of the enzyme from the cell culture. The PVA enzyme thus obtained is used in the individual production of ss-lactamic antibiotics, particularly 6-aminopenicillanic (6-APA) acid from penicillin V, although other penicillins (K, F, and dihydro-F) can also be used as a substrate