72 research outputs found
Use of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil
Brazil is one the largest producers and exporters of food commodities in the world. The evaluation of fungi capable of spoilage and the production mycotoxins in these commodities is an important issue that can be of help in bioeconomic development. The present work aimed to identify fungi of the genus Aspergillus section Flavi isolated from different food commodities in Brazil. Thirty-five fungal isolates belonging to the section Flavi were identified and characterised. Different classic phenotypic and genotypic methodologies were used, as well as a novel approach based on proteomic profiles produced by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Type or reference strains for each taxonomic group were included in this study. Three isolates that presented discordant identification patterns were further analysed using the internal transcribed spacer (ITS) region and calmodulin gene sequences. The data obtained from the phenotypic and spectral analyses divide the isolates into three groups, corresponding to taxa closely related to Aspergillus flavus, Aspergillus parasiticus, and Aspergillus tamarii. Final polyphasic fungal identification was achieved by joining data from molecular analyses, classical morphology, and biochemical and proteomic profiles generated by MALDI-TOF MS.Acknowledgments are due to FAPEMIG -
Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (Brazil)
for financial support. F. C. da Silva extends thanks to CAPES -
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Brazil)
for the PhD grant. C. Santos and N. Lima thank CAPES for the financial
support as international visiting professors in the Post-Graduate Programme
in Agricultural Microbiology, Federal University of Lavras,
Lavras (MG), Brazil
Intense quenching of fluorescence intensity of poly(vinyl pyrrolidone) molecules in presence of gold nanoparticles
Mesoporous drug carrier systems for enhanced delivery rate of poorly water-soluble drug: nimodipine
The Effects of the Composition of the Plating Bath on the Electrocrystallization of Nickel
General Models for Surface Nucleation and Three‐Dimensional Growth: the Effects of Concurrent Redox Reactions and of Diffusion
The investigation of nucleation using microelectrods. II: The second moment of the times of birth of the first nucleus
The investigation of nucleation using microelectrods. I: The ensemble averages of the times of birth of the first nucleus
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