Living in close quarters: Epibionts on Dendrophyllia ramea deep-water corals (Cyprus and Menorca Channel)

In sharp contrast to shallow and/or tropical coral habitats, the role of deep-water corals (DWC) as habitat providers is not well known and even less understood. For this purpose, epibionts on the deep-water coral Dendrophyllia ramea were studied from samples collected in Cyprus and compared to those from Menorca Channel. A total of 63 species were found; bryozoans (ca. 60%) and serpulid polychaetes (ca. 10%) dominated the assemblage of species. Cyprus (48 species in total) and Menorca (22) corals shared few epizoic species (7). Several of these species were previously thought absent from the Levantine basin. These results are important contributions to the knowledge on the deep-water epibiotic biodiversity of the Levantine Basin and the Mediterranean Sea in genera

The Deep‐water corals of Cyprus: Environmental settings and ecological features (CYprus Cold‐corals Levantine SeA, Eastern MEditerraneaN: CYCLAMEN)

The recently started research project CYCLAMEN (CYprus Cold-corals Levantine SeA, Eastern MEditerraneaN), will conduct the first detailed study of cold-water coral communities in eastern Cypriot waters. Cold-water coral habitats have been found during exploratory surveys. The 2-yr long project will include the environmental characterization of the area, as well as the study of the spatial distribution of cold-water coral communities. In addition to the study of the biology of the coral species, genetic and eco-physiological studies will be included. This project is the first of its kind in Cyprus and will additionally have an associated scientific outreach programme in order to bring these ecosystems, still poorly known, to the general public. The project is led by the Spanish Institute of Oceanography (IEO), and relies on the participation of research Institutions in Cyprus: The Cyprus Institute (CyI) and the NGO Enalia Physis Environmental Research Centre (EPERC); France: Aix-Marseille University – Mediterranean Institute for Biodiversity & Ecology (AMU-IMBE); Greece: The Hellenic Centre for Marine Research (HCMR); Mónaco: Centre Scientifique de Monaco (CSM); United Kingdom: National Oceanography Centre (NOC), and Spain: Universitat de Barcelona (UB). Here we present the conceptual frame of the project, the background knowledge and the first obtained results in the oceanographic cruise carried out in summer 2015

Occurrence and distribution of the coral Dendrophyllia ramea in Cyprus

Occurrence and abundance of deep-water corals in the Levantine Mediterranean Sea is still largely unknown. This is the first attempt to quantitatively describe a Dendrophyllia ramea population discovered in June 2015 during the CYCLAMEN expedition on board the Research Vessel Aegaeo. This population is the deepest ever described until now in the Mediterranean and was found on the outer insular shelf off eastern Cyprus (Protaras, 35°02′N; 34°05′E). Video transects conducted by means of a remotely operated vehicle revealed a well-developed population of D. ramea located on a sandy seabed at 125–170 m depth. The highest density was 6 colonies m−2 and on average 1.6 ± 1.4 (SD) colonies m−2. The population consists of isolated or piled up branches of various sizes and large colonies, some ∼50 cm max width. The corals thrive on soft bottoms, representing a rather novel aspect of the research on D. ramea, since the species is still considered to be mostly associated with rocky substrates. The occurrence of the species in sedimentary grounds makes it especially vulnerable to bottom contact fishing gears as bottom trawling. Spatial distribution of the coral population, as well as a first attempt to characterize its habitat, are explored as an approach to describe the habitat's suitability and the vulnerability for the species in the are

The “MetaCopepod” project: Designing an integrated DNA metabarcoding and image analysis approach to study and monitor the diversity of zooplanktonic copepods and cladocerans in the Mediterranean Sea

The timely and accurate analysis of marine zooplankton diversity is a challenge in ecological and monitoring studies. Morphology-based identification of taxa, which requires taxonomy experts, is time consuming and cannot provide accurate resolution at species level in several cases (e.g. immature stages, cryptic species, broken specimens). The “MetaCopepod” project is aimed at overcoming these limitations by developing a high-throughput and cost effective methodology that integrates DNA metabarcoding and image analysis. Utilizing the accuracy of DNA metabarcoding in species recognition and the quantitative results of image analysis, zooplankton diversity (mainly of copepods and cladocerans) is assessed both qualitatively (species' composition) and quantitatively (abundance, biomass and size-distribution). To achieve this goal, bulk zooplankton samples are first scanned and analyzed with ZooImage and then massively sequenced for a selected fragment of the mitochondrial 16S rRNA gene. Through a bioinformatic pipeline, sequences are compared to a reference genetic database, constructed within the project, and identified at species- level. The methodology was calibrated by using both mock and taxonomically identified samples and demonstrated on samples collected monthly from monitoring stations across the Mediterranean Sea. It is currently optimized for higher integration and accuracy and is expected to become a powerful tool for monitoring zooplankton in the long term and for early warning of bioinvasions and other ecosystem change

Testing Bergmann's rule in marine copepods

Macroecological relationships provide insights into rules that govern ecological systems. Bergmann's rule posits that members of the same clade are larger at colder temperatures. Whether temperature drives this relationship is debated because several other potential drivers covary with temperature. We conducted a near-global comparative analysis on marine copepods (97 830 samples, 388 taxa) to test Bergmann's rule, considering other potential drivers. Supporting Bergmann's rule, we found temperature better predicted size than did latitude or oxygen, with body size decreasing by 43.9% across the temperature range (-1.7 to 30ºC). Body size also decreased by 26.9% across the range in food availability. Our results provide strong support for Bergman's rule in copepods, but emphasises the importance of other drivers in modifying this pattern. As the world warms, smaller copepod species are likely to emerge as ‘winners', potentially reducing rates of fisheries production and carbon sequestration