Effect of substrate concentration on profile growth of candida tropicalis ifo 0618

This study presents effect of substrate concentration on the profile growth of Candida tropicalis IFO 0618. Different substrate concentration; D-Glucose were introduced to Candida tropicalis IFO 0618 in the Solid State Fermentation (SSF) process. The effect of substrate concentration on the profile growth of Candida tropicalis IFO 0618 was analyzed using UV-Vis Spectrophotometer and High Performance Liquid Chromatography (HPLC). The kinetic parameter (μmax) was also calculated to describe the microbial growth. From the laboratory data, the specific growth rate (μ), maximum specific growth rate (μmax) and half saturation coefficients (KS) were determined using the Monod equation. The maximum observed growth rate (μmax) for Candida tropicalis IFO 0618 was at the substrate concentration of 8.5 (g/g) with μmax value of 1.9991 hr-1, specific growth rate (μ) of 0.0821 hr-1 and half saturation coefficients (KS) of 198.4663 g/l. Result from the High Performance Liquid Chromatography also shows that there was sorbitol produced at 8.5 (g/g) substrate concentration

Effective Iron-Accumulating Bacteria Isolated from Chemical Laboratory Drainage for Iron Removal

Improperly treated heavy metal wastewater discharged into water sources could cause a serious issue for the environment. The aim of this study was to bioaccumulate iron (Fe) using native bacteria isolated from the laboratory drainage water containing a high concentration of iron. The experiment was conducted in 250 mL conical flasks containing 150 mL Fe solution in concentrations of 25, 100, and 250 mg/L, respectively. Approximately 10% of bacteria inoculum was cultivated in each Fe concentration for 24 and 48 hours. The results showed that Pseudomonas hibiscicola was identified as an effective iron-accumulating species of bacteria. The species could remove Fe up to 82% (25 mg/L), 77.8% (100 mg/L) and 32% (250 mg/L). This promising result indicates that the native bacteria isolated from the environment pose a great potential for the remediation of wastewater containing iron

Physico-chemical and Biological Techniques of Bisphenol A Removal in an Aqueous Solution

Bisphenol A (BPA) is widely used in everyday life and can be found everywhere, including in the ecosystem and manufactured goods. BPA not only has a negative impact in low doses, but it also has biological and pathophysiological implications for obesity and hormonal effects. The objectives of this paper were to review the BPA removal technology and the factors that influence the BPA removal based on biological methods. BPA elimination from water is crucial for environmental protection, in terms of biological treatment. In addition, the future prospect of biological removal of BPA indicates that effective microorganism cultures could disturb the pathogen growth and increase composition rate of BPA. The biological technology by the implementation of microorganisms for the removal of BPA through break down of organic contaminants is straightforward, money saving, and widely acknowledged by the public

Application of Response Surface Methodology for Preparation of ZnAC2/CAC Adsorbents for Hydrogen Sulfide (H2S) Capture

Hydrogen sulfide (H2S) should be removed in the early stage of biogas purification as it may affect biogas production and cause environmental and catalyst toxicity. The adsorption of H2S gas by using activated carbon as a catalyst has been explored as a possible technology to remove H2S in the biogas industry. In this study, we investigated the optimal catalytic preparation conditions of the H2S adsorbent by using the RSM methodology and the Box–Behnken experimental design. The H2S catalyst was synthesized by impregnating commercial activated carbon (CAC) with zinc acetate (ZnAc2) with the factors and level for the Box–Behnken Design (BBD): molarity of 0.2–1.0 M ZnAc2 solution, soaked temperature of 30–100 °C, and soaked time of 30–180 min. Two responses including the H2S adsorption capacity and the BET surface area were assessed using two-factor interaction (2FI) models. The interactions were examined by using the analysis of variance (ANOVA). Hence, the optimum point of molarity was 0.22 M ZnAc2 solution, the soaked period was 48.82 min, and the soaked temperature was 95.08 °C obtained from the optimum point with the highest H2S adsorption capacity (2.37 mg H2S/g) and the optimum BET surface area (620.55 m2/g). Additionally, the comparison of the optimized and the non-optimized catalytic adsorbents showed an enhancement in the H2S adsorption capacity of up to 33%