Impact of Renal of Lymphatic Hyperplasia on Blood Pressure Regulation in Male Mice

Chronic high blood pressure, or hypertension, is identified as a risk factor for heart disease, stroke, and chronic kidney disease. The primary cause of most hypertension is increased peripheral vascular resistance that is controlled, in large part, by the kidney’s water handling. In the kidney, specific immune cell subsets and overall renal inflammation have both been identified as drivers of hypertension in preclinical models. Lymphatic vessels serve as a route of both fluid and immune cell clearance and their expansion, lymphangiogenesis, is necessary for the resolution of tissue inflammation. We hypothesized that by increasing renal lymphangiogenesis, renal inflammation would be reduced and blood pressure would be normalized during salt-sensitive hypertension. To investigate the role of renal immune cell trafficking in instances of blood pressure challenge, we employed a murine model of inducible renal lymphatic expansion. Mice expressing a lymphatic growth factor, vascular endothelial growth factor (VEGF)-D, under the control of a TRE-promoter were crossed with mice expressing a Kidney Specific Protein-regulated transactivator (rtTA). Upon administration of doxycycline, the rtTA-dox complex binds to the TRE promoter region, causing transcription of VEGF-D only in renal tubular epithelial cells. The resultant kidney-specific VEGF-D overexpression caused expansion of the existing renal lymphatic network in addition to generating de novo lymphangiogenesis in the cortex of “Kid-VD” mice. We then utilized the Kid-VD mouse model during an established rodent hypertension regimen of nitric oxide inhibition and high salt diet loading to identify the impact – through weekly blood pressure measures - and mechanism – by cellular, protein, and RNA analysis – of expanded renal lymphatics on blood pressure regulation. Lymphatic circulation may thus provide a new target for the treatment of chronic hypertension and its associated co-morbidities

A Novel Approach in Pigment Printing Using Nano-keratin Based Binder

This study is devoted to preparation and characterization of nano-keratin based binder (NKBB) from cheap renewable natural resources, namely coarse Egyptian wool or feather. The prepared NKBB is utilized as a biodegradable, environment-friendly and relatively cheap binder in textile pigment printing of polyester, pure polyacrylic, viscose, polyester/viscose, and polyester/acrylic fabrics. Different concentrations of the prepared NKBB as well as its mixture with commercially produced one are used in the pigment printing paste. The colour strength of the printed fabrics as well as their fastness properties to light, washing, and perspiration are evaluated. The effect of the used binder on the bending stiffness of the printed fabrics is also assessed. Results show that the NKBB gives almost the same colour strength and fastness properties as the commercial binder with improved stiffness of the printed samples in relation to that printed with commercial one

Bone metastatic prostate cancer and resistance to tyrosine kinase inhibitors: an intimate relationship between loss of miR-203 and up-regulation of EGFR signaling: DOI: 10.14800/rd.345

A tumor suppressor role for miR-203 in RAS-dependent prostate cancer metastasis has been described recently by our group. We have explored the regulatory mechanisms by which miR-203 is being regulated through EGFR signaling. We investigated the molecular mechanism of metastasis and identified novel roles of genes that interact with miR-203 downstream of activated Ras. We showed an amplifying regulatory loop involving the direct interaction of miR-203 with the EGFR ligands, AREG, EREG, and TGFA 3’UTR. Using clinical specimens and database analysis, our data suggested that decreasing miR-203 and increasing EGFR ligands, AREG, EREG, and TGFA expressions are correlated with prostate cancer progression. Since tyrosine kinase inhibitors (TKIs) have been shown to inhibit tyrosine phosphorylation of EGFR in a dose-dependent manner, we examined a role for miR-203 in TKIs-induced apoptosis in RAS-activated prostate cancer. We investigated the mechanisms by which miR-203 overexpression contributes to TKIs-resistant RAS-activated prostate cancer cells apoptosis. We have shown indications for candidate miR-203 targets that are either influenced by anti-apoptotic proteins (e.g. API5, BIRC2, and TRIAP1) or positively influenced by a novel NF-?B-inducible oncogenic molecule, TNFAIP8. These observations suggest that the latter category may be synergistically affected by the regulatory loop of miR-203 depletion and anti-apoptotic proteins overexpression. Our results provided evidence showing a role of miR-203 in regulating the expression of EGFR signaling genes in response to TKIs-resistance during prostate cancer progression

Bone metastatic prostate cancer and resistance to tyrosine kinase inhibitors: an intimate relationship between loss of miR-203 and up-regulation of EGFR signaling

A tumor suppressor role for miR-203 in RAS-dependent prostate cancer metastasis has been described recently by our group. We have explored the regulatory mechanisms by which miR-203 is being regulated through EGFR signaling. We investigated the molecular mechanism of metastasis and identified novel roles of genes that interact with miR-203 downstream of activated Ras. We showed an amplifying regulatory loop involving the direct interaction of miR-203 with the EGFR ligands, AREG, EREG, and TGFA 3’UTR. Using clinical specimens and database analysis, our data suggested that decreasing miR-203 and increasing EGFR ligands, AREG, EREG, and TGFA expressions are correlated with prostate cancer progression. Since tyrosine kinase inhibitors (TKIs) have been shown to inhibit tyrosine phosphorylation of EGFR in a dose-dependent manner, we examined a role for miR-203 in TKIs-induced apoptosis in RAS-activated prostate cancer. We investigated the mechanisms by which miR-203 overexpression contributes to TKIs-resistant RAS-activated prostate cancer cells apoptosis. We have shown indications for candidate miR-203 targets that are either influenced by anti-apoptotic proteins (e.g. API5, BIRC2, and TRIAP1) or positively influenced by a novel NF-κB-inducible oncogenic molecule, TNFAIP8. These observations suggest that the latter category may be synergistically affected by the regulatory loop of miR-203 depletion and anti-apoptotic proteins overexpression. Our results provided evidence showing a role of miR-203 in regulating the expression of EGFR signaling genes in response to TKIs-resistance during prostate cancer progression

Nurses' Knowledge, Attitude and Practices of Nursing Informatics System in Outpatient Clinics of Children's Cancer Hospital (57357) at Cairo

Technology in health care is increasingly becoming an integral part of the Egypt. Health care delivery system and is declared by strategists as a means whereby sustained improvement in health-care outcomes may be attained. This study was carried out aiming to assess nurses` Knowledge, attitude and practice about the nursing informatics system at out -patient clinics of Children's Cancer Hospital (57357), at Cairo. A descriptive research design will be utilized in this study was carried out in this hospital during April 2013. Sample consists of nineteen male and female nurses who are working in out-patient clinics. This study was conducted at the different out-patient clinics at Children Cancer Hospital (57357) at Cairo. Two tools were utilized to collect data pertinent to the study; one of them is a socio demographic data sheet and the second tool is a structured interview questioner schedule developed by investigator. Findings of this study showed that the majority of the sample was females and more than half of them were single. In addition to, it was found that, more than half of them academic faculties. Also, there was highly statistical significant difference between total knowledge of nursing informatics system according to total practice and total attitude of nursing informatics system. This study concluded that nurses are responsible for a substantial part of the patient record and hence are particularly affected by the computerization. The appliance of information system into nursing provides important advantages of the nursing personnel’s data and contributing to the improvement of the operating effectiveness of the nursing service. Recommendation: In Egypt (2013), nursing informatics is a new specialty and therefore should cover major sectors of the healthcare system where nurses work; this includes the clinical, administrative, research and education areas. Keywords: Knowledge, Attitude and practices nursing informatics, Nursing Informatics System, Hospital Information System

Flexural resistance of the polypropylene fibres reinforced cement mixes with waste material

The polypropylene (PP) fibers in shotcrete has been used for ground support and building strengthening, since several decades. However, the recent trend is to use the waste material in cementbased mixes to produce an eco-friendly material. Such waste material is the incineration fly ash (FA) that is classified as a hazardous product. This study is intended to establish the mechanical properties of fiber reinforced mortar in addition to cement or sand partial replacement by fly ash, in terms of flexural strength testing. The mechanical properties reflect the influence of the dosage of fiber content and the proportion of the fly ash on the flexural strength. The percentage of cement or sand was replaced by 0, 10, 20, and 30% fly ash. The dosage of fibers was 0, 0.6, 1.2, and 1.8 kg/m3. This green mix with fibers provides a partial substitute of cement as it is cheaper, by incorporating waste product, and saving energy consumption in the production. Due to growing interest in sustainable construction, engineers and architects are motivated to choose such materials which are more sustainable

Potentiometric titration of reducing carbohydrates IV. : application of sulphide-selective electrode in sugar analysis

A javasolt módszer abból áll, hogy a redukáló cukrot 0,1 m Hg(II)-EDTA oldattal melegítik. A reagálatlan Hg(II) mennyiségét úgy határozzák meg, hogy az oldathoz szűrés nélkül fölös mennyiségben adnak 0,1 m nátrium-szulfid oldatot és a szulfidfölösleget (0,1 m Hg(II)-EDTA oldattal visszatitrálják. A végpontot szulfidszelektív elektród alkalmazásával potenciometriásan határozzák meg. Ha a melegítést vízfürdőn végzik, a reakció a Hg(II) és a cukor között 30 perc alatt befejeződik. 12 — 42 mg közötti redukáló cukor mennyiségek 10 cm3 0,1 m Hg(II)-EDTA oldattal +1 — 2% átlagos hibával meghatározhatók. Fémhigany jelenléte nem zavarja az eljárást. Die vorgeschlagene Methode besteht aus Erwärmung des reduzierenden Zuckers mit einer 0,1 m Hg(II)-EDTE-Lösung. Unverbrauchtes Hg(II) wird dann —ohne Filtrierung— durch Zugabe einer überschüssigen 0,1 m Natriumsulfidlösung und Zurücktitrierung des Sulfidüberschusses mit einer 0,1 mHg(II)- EDTE-Lösung bestimmt. Die Endpunktbestimmung erfolgt potentiometrisch, unter Anwendung einer sulfidselektiven Elektrode. Wird die Erwärmung aif einem Wasserbad durchgeführt, so beendet sich die Reaktion zwischen Hg(II) und dem reduzierenden Zucker binnen 30 Minuten. Eine Menge von 12-42 mg von reduzierendem Zucker wird mit 10 ml 0,1 m Hg(I I)-EDTE-Lösung mit einem durchschnittlichen Fehler von + 1—2% bestimmt. Anwesenheit von metallischem Quecksilber hat keinen störenden Einfluss. The suggested method consists in heating the reducing sugar with a 0.1 M Hg(II)-EDTA solution. Unconsumed Hg(II) is then determined without filtration, by adding an excess of 0.1 M sodium sulphide solution and back-titrating excess sulphide with aO.l M Hg(II)-EDTA solution. The end point is detected by potentiometry, using a sulphide-selective electrode. If heating is carried out on the water bath, the reaction between Hg(II) and sugar is completed in 30 minutes. Amounts of 12 — 42 mg of reducing sugar are determined with 10 ml of 0.1 M Hg(II)-EDTA solution with an average error of +1 — 2%. The method is not affected by the presence of metallic mercury. Selon la méthode proposée on chauffe le sucre réducteur avec une 0,1 M solution Hg(II)-EDTA. On effectue le dosage du Hg(II) non réagi en ajoutant ä la solution, sans filtrer, un excés d’une solution 0,1 M de Na.S, ensuite on effectue la titration de l’excés du sulphide avec la solution 0,1 M de Hg(II)-EDTA. On déterinine le point final par potentiométrie, ä l’aide d’une electrode sulphideselective. En effectuant le chauffage dans un bain-marie, la reaction entre le Hg(II) et le sucre se termine en 30 minutes. Des quantités de sucre réducteur de 12 ä 42 mg se font déterminer avec 10 ml de la solution 0,1 M de Hg(II)-EDTA avec une erreur de ± 1 —2%. La présence de mercure métallique n’interfére pas avec la méthode

REVISITING PREHISTORIC ARCHEOLOGICAL SITES: ENVISIONING FIRST BUILT ENVIRONMENTS TO REPOSSESS GEOGRAPHICALLY SPECIFIC APPROACHES IN ARCHITECTURE

Since Prehistoric times, architecture had been a human response to an occurring natural setting. Starting from places of dwelling to buildings that no longer only serve physical requirements for survival. Architectural languages were approached initially as an expression of culture, evolution, and growth of a community within a natural setting. This response resulted in the creation of built environments, humanity’s decision to become sedentary. This decision took place in the Late Stone age, a key phase in our timeline. First built environments were born in a time known as the Neolithic revolution, which shown itself as humans transitioned from hunter-gatherer societies to agrarian based ones. Once Nature shaped man, now man shapes nature. We observe the dynamic created between man the hosting setting. While observing this two sided complex operating system through revisiting prehistoric archeological sites, we can notice how various geographic zones birthed a diversity of built environments. However, by observing the paradigm of duality in our modern world, we can see neglected neighborhoods and cities, and more importantly that we failed to fulfill our fair part of shaping our natural setting and this reflected on the state of our communities as well. By repossessing how to be geographically specific in architecture, we can set the parameters to architectural planning that includes nature as a co-partner and as a result attempt to improve the well-being of our neglected communities

Derivatographic detection of pig's fat in other animal fats

Egyszerű és gyors módszert írnak le a sertészsír kimutatására más állati zsírokban vagy hidrogénezett növényi olajokhoz keverten. Az eljárásnál nincs szükség előzetes elválasztásra, és alkalmasnak bizonyult a sertészsír jelenlétének kimutatására egyes importált húskonzervekben. Eine einfache und rasche Methode wird zum Nachweis des Schweinefet ts in anderen tierischen Fetten oder in Fettgemischen m it hydrierten Pflanzenöl en eschrieben. Bei der Methode wird keine vorangehende Abtrennung benöti gt, und dieMethode erwies sich als geeignet, in einigen importierten Fleischkons erven die Gegenwart von Schweinefleisch nachwuweisen