Efecto de la fertilización con potasio y de la aplicación foliar de zinc y fósforo en el rendimiento de aceite y proteína y en las propiedades del aceite de algodón

In maximizing the quantity and quality of a crop’s nutritional value in terms of fatty acids and protein, it is necessary to identify the constraints which may affect it and to devise methods of overcoming them through the use of inputs or changes in management practices. Field experiments were conducted during two successive seasons at the Agricultural Research Center, Giza, Egypt, on the cotton cultivar “Giza 86” (Gossypium barbadense L.) to study the effects of potassium fertilization (at 0.0 and 47.4 kg of K ha–1) and foliar application of zinc (at 0.0 and 57.6 g of Zn ha–1, two times, 70 and 85 days after planting, “during square initiation and boll setting stage”) and phosphorus (at 0.0, 576, 1152 and 1728 g of P ha–1, two times, 80 and 95 days after planting) on cottonseed. The application of potassium along with spraying plants with zinc and phosphorus caused  an increase in cottonseed yield ha–1, seed index, seed oil content, oil and protein yields ha–1, seed oil unsaponifiable matter and total unsaturated fatty acids (oleic and linoleic). However, those treatments resulted in a decrease in oil acid value, saponification value and total saturated fatty acids. The highest P concentration of 1728 g ha–1 gave the best values of cottonseed yield/ha, seed index, and seed oil and protein yields/ha and oil saponifiable matter.Para maximizar la cantidad y calidad del valor nutricional de una semilla en términos de ácidos grasos y proteínas es necesario identificar los factores que los afectan y proponer métodos que favorezcan los resultados deseados a través de cambios o mejoras en las prácticas utilizadas. Los experimentos se llevaron a cabo en dos campañas sucesivas en el Agricultural Research Center, Giza, Egipto, en el cultivo “Giza 86” (Gossypium barbadense L.) para estudiar los efectos de la fertilización con potasio (a 0.0 y 47.7 kg por ha) y las aplicaciones foliares de zinc (a 0.0 y 57.6 g por ha, dos veces, 70 y 85 días después de la plantación) y fósforo (a 0.0, 576, 1152, y 1728 g por ha, dos veces, 80 y 95 días después de la plantación). La aplicación del potasio junto con la pulverización de las plantas con zinc y fósforo causó un incremento en el rendimiento del algodón, el índice de semilla, el contenido graso, los rendimientos de aceite y proteína, el contenido de materia insaponificable en el aceite y el contenido total de ácidos grasos insaturados (oleico y linoleico). Por el contrario estos tratamientos disminuyeron el índice de acidez, el índice de saponificación y el contenido de ácidos grasos saturados. La mayor concentración de fósforo produjo los mejores rendimientos de algodón, de índice de semilla, de los rendimientos de aceite y proteína, y de materia insaponificable en el aceite

Molecular Regulation of Muscle Protein Synthesis at Rest and Following Endurance Exercise

The purpose of this study was to investigate whether protein consumption after endurance exercise modulates postprandial fraction-specific muscle protein synthesis. In crossover trials, eight trained men received a primed continuous infusion of L-[ring-2H5]phenylalanine to measure changes in myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis in response to 20g of egg protein with 50 g carbohydrates at rest and after 1 h of treadmill running at 70%VO2peak. Muscle biopsies were obtained while fasted and at 0, 1 and 5h after beverage ingestion to determine Myo/MitoPS (LC/MS/MS) and intracellular signaling (Western blotting/immunofluorescence). We demonstrate that MitoPS is uninfluenced by feeding and exercise whereas MyoPS is primarily nutrient sensitive over a 5h postprandial period at rest and after endurance exercise, which may be underpinned in part by enhanced mRNA translational capacity mediated through an increase in 4E-BP1 phosphorylation and secondary to an increase in mTOR/Rheb colocalization and reciprocal decrease in TSC2/Rheb colocalization.M.Sc

‘Engineering physiology’ to understand the post-exercise biochemical milieu: mixing it up with anabolic hormones

Corum (conference center, 1988); Montpellier was one of the pilgrimage sites on route to the Cathedral of Santiago de Compostela. It is famous for the terraced 17th- and 18th-century Promenade du Peyrou which offers spectacular views. Montpellier is the site of the oldest Botanical Gardens in France which was founded by Henry IV in 1593. It has an important French paintings museum called the Fabre Museum and is the seat of International Vine and Wine Fair. Historic sites include city walls, early churches, and hospitals. Source: Thesaurus of Geographic Names (Notes) [website]; http://www.getty.edu/research/conducting_research/vocabularies/tgn/ (accessed 7/14/2010

Whey Protein Supplementation Enhances Whole Body Protein Metabolism and Performance Recovery after Resistance Exercise: A Double-Blind Crossover Study

No study has concurrently measured changes in free-living whole body protein metabolism and exercise performance during recovery from an acute bout of resistance exercise. We aimed to determine if whey protein ingestion enhances whole body net protein balance and recovery of exercise performance during overnight (10 h) and 24 h recovery after whole body resistance exercise in trained men. In a double-blind crossover design, 12 trained men (76 ± 8 kg, 24 ± 4 years old, 14% ± 5% body fat; means ± standard deviation (SD)) performed resistance exercise in the evening prior to consuming either 25 g of whey protein (PRO; MuscleTech 100% Whey) or an energy-matched placebo (CHO) immediately post-exercise (0 h), and again the following morning (~10 h of recovery). A third randomized trial, completed by the same participants, involving no exercise and no supplement served as a rested control trial (Rest). Participants ingested [15N]glycine to determine whole body protein kinetics and net protein balance over 10 and 24 h of recovery. Performance was assessed pre-exercise and at 0, 10, and 24 h of recovery using a battery of tests. Net protein balance tended to improve in PRO (P = 0.064; effect size (ES) = 0.61, PRO vs. CHO) during overnight recovery. Over 24 h, net balance was enhanced in PRO (P = 0.036) but not in CHO (P = 0.84; ES = 0.69, PRO vs. CHO), which was mediated primarily by a reduction in protein breakdown (PRO < CHO; P < 0.01. Exercise decreased repetitions to failure (REP), maximal strength (MVC), peak and mean power, and countermovement jump performance (CMJ) at 0 h (all P < 0.05 vs. Pre). At 10 h, there were small-to-moderate effects for enhanced recovery of the MVC (ES = 0.56), mean power (ES = 0.49), and CMJ variables (ES: 0.27–0.49) in PRO. At 24 h, protein supplementation improved MVC (ES = 0.76), REP (ES = 0.44), and peak power (ES = 0.55). In conclusion, whey protein supplementation enhances whole body anabolism, and may improve acute recovery of exercise performance after a strenuous bout of resistance exercise

Leucine-Enriched Essential Amino Acids Improve Recovery from Post-Exercise Muscle Damage Independent of Increases in Integrated Myofibrillar Protein Synthesis in Young Men

Background: Leucine-enriched essential amino acids (LEAAs) acutely enhance post-exercise myofibrillar protein synthesis (MyoPS), which has been suggested to be important for muscle repair and recovery. However, the ability of LEAAs to concurrently enhance MyoPS and muscle damage recovery in free-living humans has not been studied. Methods: In a randomized, double-blind, placebo-controlled, parallel-group design, twenty recreationally active males consuming a controlled diet (1.2 g/kg/d of protein) were supplemented thrice daily with 4 g of LEAAs (containing 1.6 g leucine) or isocaloric placebo for four days following an acute bout of lower-body resistance exercise (RE). MyoPS at rest and integrated over 96 h of recovery was measured by D2O. Isometric and isokinetic torque, muscle soreness, Z-band streaming, muscle heat shock protein (HSP) 25 and 72, plasma creatine kinase (CK), and plasma interleukin-6 (IL-6) were measured over 96 h post-RE to assess various direct and indirect markers of muscle damage. Results: Integrated MyoPS increased ~72% over 96 h after RE (p < 0.05), with no differences between groups (p = 0.98). Isometric, isokinetic, and total peak torque decreased ~21% by 48 h after RE (p < 0.05), whereas total peak torque was ~10% greater overall during recovery in LEAAs compared to placebo (p < 0.05). There were moderate to large effects for peak torque in favour of LEAAs. Muscle soreness increased during recovery with no statistical differences between groups but small to moderate effects in favour of LEAAs that correlated with changes in peak torque. Plasma CK, plasma IL-6, and muscle HSP25 increased after RE (p < 0.05) but were not significantly different between groups (p ≥ 0.13). Consistent with a trend toward attenuated Z-band streaming in LEAAs (p = 0.07), muscle HSP72 expression was lower (p < 0.05) during recovery in LEAAs compared with placebo. There were no correlations between MyoPS and any measures of muscle damage (p ≥ 0.37). Conclusion: Collectively, our data suggest that LEAAs moderately attenuated muscle damage without concomitant increases in integrated MyoPS in the days following an acute bout of resistance exercise in free-living recreationally active men

Leucine-Enriched Essential Amino Acids Improve Recovery from Post-Exercise Muscle Damage Independent of Increases in Integrated Myofibrillar Protein Synthesis in Young Men

Background: Leucine-enriched essential amino acids (LEAAs) acutely enhance post-exercise myofibrillar protein synthesis (MyoPS), which has been suggested to be important for muscle repair and recovery. However, the ability of LEAAs to concurrently enhance MyoPS and muscle damage recovery in free-living humans has not been studied. Methods: In a randomized, double-blind, placebo-controlled, parallel-group design, twenty recreationally active males consuming a controlled diet (1.2 g/kg/d of protein) were supplemented thrice daily with 4 g of LEAAs (containing 1.6 g leucine) or isocaloric placebo for four days following an acute bout of lower-body resistance exercise (RE). MyoPS at rest and integrated over 96 h of recovery was measured by D2O. Isometric and isokinetic torque, muscle soreness, Z-band streaming, muscle heat shock protein (HSP) 25 and 72, plasma creatine kinase (CK), and plasma interleukin-6 (IL-6) were measured over 96 h post-RE to assess various direct and indirect markers of muscle damage. Results: Integrated MyoPS increased ~72% over 96 h after RE (p < 0.05), with no differences between groups (p = 0.98). Isometric, isokinetic, and total peak torque decreased ~21% by 48 h after RE (p < 0.05), whereas total peak torque was ~10% greater overall during recovery in LEAAs compared to placebo (p < 0.05). There were moderate to large effects for peak torque in favour of LEAAs. Muscle soreness increased during recovery with no statistical differences between groups but small to moderate effects in favour of LEAAs that correlated with changes in peak torque. Plasma CK, plasma IL-6, and muscle HSP25 increased after RE (p < 0.05) but were not significantly different between groups (p ≥ 0.13). Consistent with a trend toward attenuated Z-band streaming in LEAAs (p = 0.07), muscle HSP72 expression was lower (p < 0.05) during recovery in LEAAs compared with placebo. There were no correlations between MyoPS and any measures of muscle damage (p ≥ 0.37). Conclusion: Collectively, our data suggest that LEAAs moderately attenuated muscle damage without concomitant increases in integrated MyoPS in the days following an acute bout of resistance exercise in free-living recreationally active men