28 research outputs found
Effects of ethanolic leaf extract of Chrysophyllum albidum G. on biochemical and haematological parameters of albino Wistar rats
The effect of oral administration of the leaf extract of Chrysophyllum albidum G. on biochemical and haematological parameters were investigated in albino rats for 16 days. The extract did not show any significant effect (p > 0.05) on the plasma concentrations of total bilirubin, albumin and alkaline phosphatase (ALP) as well as the packed cell volume (PCV), haemoglobin (Hb), red blood cell (RBC), reticulocytes, neutrophils, lymphocytes, eosinophils, basophils, mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular haemoglobin (MCH). The concentration of the platelets was significantly decreased (p < 0.05) at 1000 mg/kg body weight, while white blood cell (WBC) was significantly increased at 500 mg/kg body weight. The doses significantly reduced (p < 0.05) plasma levels of AST, ALT, total protein, glucose and creatinine while urea was significantly increased. While the extract significantly increased the lung, brain and liver-body weights, the kidney, heart, testis, spleen and epididymis-body weights were not significantly affected. The result suggests that the leaf extract of C. albidum contains antiplatelet and hypoglycemic properties and exhibited selective organ toxicity to the rats
Genetic diversity of Plasmodium falciparum field Isolates from South western Nigeria
Abstract Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria
Genetic diversity of Plasmodium falciparum field isolates from south western Nigeria
Background: Plasmodium falciparum the main causative agent of malaria
is an important public health vector. With the use of PCR, its genetic
diversity has been extensively studied with dearth information from
Nigeria. Methods: In this study, 100 P. falciparum strains merozoite
surface protein 1( msp-1), merozoite surface protein 2 (msp-2) and
Glutamate rich protein (Glurp) from Ogun State General Hospitals were
characterized. The genetic diversity of P. falciparum isolates was
analyzed by restriction fragment length polymorphism following gel
electrophoresis of DNA products from nested polymerase chain reactions
(PCR) of their respective allelic families KI, MAD 20, RO33
(MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively.
Results: Majority of the patients showed monoclonal infections while
multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2
respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3
MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for
Glurp with allelic size ranged between 700 and 900 bp. Conclusion: The
allelic distributions however were similar to those previously reported
in other endemic malaria countries. Future studies will be designed to
include other malaria endemic regions of Nigeria such as the oil
exploration regions
Genetic diversity of Plasmodium falciparum field isolates from south western Nigeria
Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria.Methods: In this study, 100 P. falciparum strains merozoite surface protein 1( msp-1), merozoite surface protein 2 (msp-2) and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P. falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively.Results: Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp.Conclusion: The allelic distributions however were similar to those previously reported in other endemic malaria countries. Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration regions.Keywords: Genetic diversity; Plasmodium; Merozoite surface protein, monoclonal infectio