Some Microbial and Immunophysiological Parameter in Patients with Renal Failure

Background: Escherichia coli reported  to be the  most common  bacteria isolate from patients with renal failure, while Klebsilla pneumoniae. were found  to  be  the  second common isolated, followed  by Staphlococcus aureus.Also study explained an increased in levels of IL-17,FasL, ,creatinine,urea,.Objectives: To isolate  microorganisms  from  patients  with  renal failure, also to determined Immunphsiological  parameter  for patients.Methods:102 blood sample (61 male (59.8%) and 41female (40.1%)) , 150 urine sample for pateints  underogoing hemodialysis at industrial kidney unit in Marjan Teaching Hospital from November 2013 to June 2014.All sample were cultured on different media for full bacteriological identification . In addition blood samples were taken from patients for immunphysiological tests.Results:The most common gram negative bacterial isolates were Escherichiae coli (38.8%), followed Klebsiella pneumoniae (34.32%), Enterobacter spp (6.71%) and Pseudomonas aerogenosa (4.47%).The gram positive bacteria were Staphylococcus aureus (13.43%) and Staphylococcus epidermidis (2.24%).Also results explained an increased in levels of IL-17, Fas L, , creatinine , urea, Keywords :IL17 , Fas-L , Bacteria ,S. creatinine ,B. ure

Expression of Pro Inflammatory Cytokines and Anti-Chlamydia Antibody in Bacterial Ocular Infection.

Current study was done to investigate the bacterial causes of eye infection for 130 patients with different ages in Babylon city , the study includes swab for eye and sample of blood. Bacteriological diagnostic  for eye swab which taken from different site include Conjunctivitis, Dacryocystitis, Lidinfection showed variation in types and number of isolates . Gram positive bacterial isolates include Staphylococcus aureus 67 (38.06%), Which represent the predominating bacteria, Staphylococcus epidermidis 18 (10.22%), While gram negative bacteria represent Escherichia coli 40 (22.7%), Neisseriae gonorrhoeae 5 (2.84%). Detection of Chlamydia trachomatis reveals only one positive case  out of 42 eye infected case for anti Chlamydia IgG –IgM ( 3.7 %), Immunological test of Cytokine IL-6 in patient show increased in their concentration compared with healthy at P< 0.05 reached 8.52 pg/ml especially at age group 41-50. IFN-? also show increased in their concentration in patient than controls although, there is no significant differences between patient and control groups. . Key words ; IFN gamma , IL-6 , eye infection , bacterial isolates ,Chlamydia trachomati

Partially Purification M Protein from Streptococcus Pyogens and Molecular Study of Some Virulence Factors

The current study was conducted to isolation one type of surface protein of Streptococcus pyogens this protein separated using partially purification method. Purifiction  process include precipitation of crude  M protein  one peak of this protein was appeared, the best precipitate rate was 30% . PCR assay were performed to identify the presence of some genes related to pathogenicity in bacteria responsible from Tonsillitis .Streptococcus pyogens represented the largest causative agent of inflammation of the tonsil so the study revealed the presence of certain gene in some isolate of bacteria, including the gene SpeB that gave large proportions in the study isolates 12 out of 37 at a rate of (32.4%). Keywords : S.pyogens , Tonsillitis , M protein ,PCR ,Spe

Evaluation of microRNA and some immunological factors associated with SARS-CoV-2 infection

Coronavirus (CoV) is a family of RNA viruses that cause disease in humans and other animals by entering the body via ACE2 receptors found in various organs such as the heart, lungs, kidneys, and digestive system. Coronaviruses are large particle viruses that are enveloped, spherical, have surface protrusion-forming spikes, and have a large positive- sense single-stranded RNA genome. A total 90 samples were collected from people suspected of being infected with SARSCoV-2, 50 blood samples were collected from patients, and 40 samples were collected from healthy individuals as a control group, who aged 20-80 years and carried out during September (2020) to January (2022) at Al-Hussein Teaching Hospital. All were checked by using real- time quantitative polymerase chain reaction (RTPCR) technology. Evaluations of miRNAs were performed in a group of newly infected patients with the SARSCoV-2 virus and healthy individuals who were cured, the result found MiRNA -192 gene have 84 % positive RT-PCR result in comparison with 16 % in which have negative result , while the other gene MiRNA - 31 have 36% positive result in comparison with 64% as a negative result, as well as 100 % of H.K. gene