1 research outputs found
Layer-by-Layer Polymer Coated Gold Nanoparticles for Topical Delivery of Imatinib Mesylate To Treat Melanoma
The aim of this study was to investigate
the feasibility of using
layer-by-layer polymer coated gold nanoparticles (AuNP) as a carrier
for topical iontophoretic delivery of imatinib mesylate (IM). AuNP
were prepared by the Turkevich method and were stabilized and functionalized
using polyvinylpyrrolidone and polyethylene imine. The functionalized
AuNP were then sequentially coated with anionic poly(styrenesulfonate)
and cationic polyethylene imine and loaded with IM. The layer-by-layer
polymer coated AuNP (LbL-AuNP) showed average particle size and zeta-potential
of 98.5 ± 4.3 nm and 32.3 ± 1.3 mV respectively. After LbL
coating of AuNP, the surface plasmon resonance wavelength shifted
from 518 to 530 nm. The loading efficiency of IM in LbL-AuNP was found
to be 28.3 ± 2.3%, which was greatest for any small molecule
loaded in AuNP. <i>In vitro</i> skin penetration studies
in excised porcine ear skin showed that iontophoresis (0.47 mA/cm<sup>2</sup>) application enhanced the skin penetration of IM loaded AuNP
by 6.2-fold compared to passive application. Tape stripping studies
showed that iontophoresis of IM loaded LbL-AuNP retained 7.8- and
4.9-fold greater IM in stratum corneum and viable skin respectively
compared with iontophoresis of free IM. LbL-AuNP were taken up rapidly
(15 min) by B16F10 murine melanoma cells. Furthermore, IM loaded LbL-AuNP
significantly (<i>p</i> < 0.001) decreased B16F10 cell
viability compared to free IM. We have shown for the first time that
IM can be delivered by topical application using LbL coated gold nanoparticles
to treat melanoma