Detection of Lung Cancer: Concomitant Volatile Organic Compounds and Metabolomic Profiling of Six Cancer Cell Lines of Different Histological Origins

In recent years, there has been an extensive search for a non-invasive screening technique for early detection of lung cancer. Volatile organic compound (VOC) analysis in exhaled breath is one such promising technique. This approach is based on the fact that tumor growth is accompanied by unique oncogenesis, leading to detectable changes in VOC emitting profile. Here, we conducted a comprehensive profiling of VOCs and metabolites from six different lung cancer cell lines and one normal lung cell line using mass spectrometry. The concomitant VOCs and metabolite profiling allowed significant discrimination between lung cancer and normal cell, nonsmall cell lung cancer (NSCLC) and small cell lung cancer (SCLC), as well as between different subtypes of NSCLC. It was found that a combination of benzaldehyde, 2-ethylhexanol, and 2,4-decadien-1-ol could serve as potential volatile biomarkers for lung cancer. A detailed correlation between nonvolatile metabolites and VOCs can demonstrate possible biochemical pathways for VOC production by the cancer cells, thus enabling further optimization of VOCs as biomarkers. These findings could eventually lead to noninvasive early detection of lung cancer and differential diagnosis of lung cancer subtypes, thus revolutionizing lung cancer treatment

Investigating the Role of Copper Oxide in Electrochemical CO₂ Reduction in Real Time

Copper oxides have been of considerable interest as electrocatalysts for CO₂ reduction (CO2R) in aqueous electrolytes. However, their role as an active catalyst in reducing the required overpotential and improving the selectivity of reaction compared with that of polycrystalline copper remains controversial. Here, we introduce the use of selected-ion flow tube mass spectrometry, in concert with chronopotentiometry, in situ Raman spectroscopy, and computational modeling, to investigate CO2R on Cu₂O nanoneedles, Cu₂O nanocrystals, and Cu₂O nanoparticles. We show experimentally that the selective formation of gaseous C₂ products (i.e., ethylene) in CO2R is preceded by the reduction of the copper oxide (Cu₂OR) surface to metallic copper. On the basis of density functional theory modeling, CO2R products are not formed as long as Cu₂O is present at the surface because Cu₂OR is kinetically and energetically more favorable than CO2R