Uklanjanje kolodijskog ugljika iz krvi tilapije (Oreochromis niloticus L.).

This study reports the rate of clearance of colloidal carbon from the blood of Tilapia (Oreochromis niloticus L.), over a period of 250 minutes following intravenous injection of five different doses. Thirty Tilapias (125-170g) were divided into 6 equal groups (A-F). Carbon doses of 4 mg, 8 mg, 16 mg, 32 mg and 48 mg were injected to fish in 0.15 ml distilled water. The control, group F, was injected with 0.15 ml distilled water. The clearance of carbon was observed to be regular and progressive for all doses. Carbon particles uptake by monocytes was noticed 5 minutes post-injection. However, the phagocytic index was inversely proportional to the dose of carbon injected. The significance of clearance of carbon and other particulate antigens is discussed.Istražen je stupanj uklanjanja intravenski ubrizganih različitih doza kolodijskog ugljika iz krvi tilapije (Oreochromis niloticus L.) tijekom 250 minuta. Trideset riba (125-170 g) bilo je svrstano u šest skupina (A-F). Ugljik u dozi 4 mg, 8 mg, 16 mg, 32 mg i 48 mg bio je ubrizgan s 0,15 ml destilirane vode po ribi. Kontrolnoj skupini je bilo ubrizgano 0,15 ml destilirane vode. Potvrđeno je redovito uklanjanje bez obzira na količinu ubrizganog ugljika. Uklanjanje ugljika monocitima potvrđeno je već nakon 5 minuta iako je indeks fagocitoze bio obrnuto razmjeran dozi ubrizganog ugljika. Raspravlja se o značenju ukljanjanju ugljika i drugih stranih tvari

Presence of trypanosome species and anemic status of dogs in Zuru, Nigeria

The aim of this research is to study the presence and prevalence of trypanosome species in local dogs between January and July, 2010 in the Zuru area of Kebbi State, Nigeria.Standard trypanosome detection methods comprising of wet blood films, thin films and microhaematocrit centrifugation technique were used to detect trypanosomes; while the degree of anemia was determined through the use of FAMACHA® eye colour chart and packed cell volume values. A total of 567 dogs were enumerated in fourteen locations within the study area out of which 192 (33.7%) were randomly examined and 4 (2.08%) were positive for the presence of trypanosomes. All positive samples morphologically belong to the Trypanosoma brucei group. The obtained PCV values showed that 50 (26.04%) dogs were anemic, while the FAMACHA® detected anemia status of varying degrees in 104 (77%) sampled dogs.These findings are significant as this is the first time that the trypanosome infection will be reported in dogs from the study area. This study establishes the presence of Trypanosoma brucei group in the study area, which is of zoonotic and economic importance