3 research outputs found
Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals.
We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for the detection of nucleocapsid and spike antibodies specific to the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and dried blood spots (DBS)
A Rapid and Quantitative Serum Test for SARS-CoV-2 Antibodies with Portable Surface Plasmon Resonance Sensing
We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies
Cross-Validation of ELISA and a Portable Surface Plasmon Resonance Instrument for IgG Antibodies Serology with SARS-CoV-2 Positive Individuals
We
report on the development of surface plasmon resonance (SPR) sensors and
matching ELISAs for the detection of nucleocapsid and spike antibodies specific
against the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and
dried blood spots (DBS). When exposed to SARS-CoV-2 or a vaccine against
SARS-CoV-2, the immune system responds by expressing antibodies at levels that
can be detected and monitored to identify the fraction of the population
potentially immunized against SARS-CoV-2 and support efforts to deploy a
vaccine strategically. A SPR sensor coated with a peptide monolayer and
functionalized with various sources of SARS-CoV-2 recombinant proteins expressed
in different cell lines detected human anti-SARS-CoV-2 IgG in the nanomolar
range. Nucleocapsid expressed in different cell lines did not significantly change
the sensitivity of the assays, whereas the use of a CHO cell line to express
spike ectodomain led to excellent performance. This bioassay was performed on a
portable SPR instrument capable of measuring 4 biological samples within 30
minutes of sample/sensor contact and the chip could be regenerated at least 9
times. Multi-site validation was then performed with in-house and commercial
ELISA, which revealed excellent cross-correlations with Pearson’s coefficients
exceeding 0.85 in all cases, for measurements in DBS and plasma. This strategy
paves the way to point-of-care and rapid testing for antibodies in the context
of viral infection and vaccine efficacy monitoring