Expression Of Phac1 And Phac2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System [QR82.P78 B195 2007 f rb].

Polihidrosialkanoat (PHA) disintesiskan secara intrasel dalam pelbagai mikroorganisma sebagai bahan simpanan untuk sumber karbon dan tenaga. PHA sintase (PhaC) merupakan enzim utama dalam pempolimeran PHA. Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs

Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate

Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupriavidus necator. A broad-host range plasmid pBBR1MCS-2 was modified to construct an expression plasmid pYEB-100, which was designed to facilitate cloning of PHA synthases in order to assess the performance of various PhaCs and the composition of PHA accumulated in a standard environment

Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System

Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs. Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend of short chain length and medium chain length of polymer. Genes coding for PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified (each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for sequencing and then expressed. For expression studies, pQE-30 was used as the expression vector under the control of PT5 promoter. These clones were named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside (IPTG) at ODeoo of 0.6

Potential Hazards at the Construction Workplace due to Temporary Structures

AbstractScaffolds are types of temporary structures which contributes towards the accident occurences at the construction workplace. Thus, the root causes should be identified to avoid unpredicted events at the workplace. This study is to investige the level of awareness amongst the contractors due to scaffold's potential hazards whichlead to injuries and fatalities at the workplace. View from Project Manager, Safety and Health Officer and Site Engineer is been focussed by using structured questionnaire. The findings indicate that awareness level amongst these profesionalstowards this issue is high.Thus, by increasing level of awareness, it will decrease number of accidents