Dynamic cell and microparticle control via optoelectronic tweezers

Abstract—This paper reports on cell and microparticle manipulation using optically induced dielectrophoresis. Our novel optoelectronic tweezers (OET) device enables optically controlled trapping, transportation, and sorting via dielectrophoretic forces. By integrating a spatial light modulator and using direct imaging, arbitrary dynamic manipulation patterns are obtained. Here, we demonstrate manipulation functions, including particle collectors, single-particle traps, individually addressable single-particle arrays, light-defined particle channels, and size-based particle sorting. OET-induced particle manipulation velocities are analyzed as a function of the applied voltage, optical pattern linewidth, and single-particle trap dimensions. [2006-0210] Index Terms—Dielectrophoresis (DEP), optical tweezers, optically induced DEP, optoelectronic tweezers (OET). I