7 research outputs found
Early onset preeclampsia is characterized by altered placental lipid metabolism and a premature increase in circulating FABP4
Preeclampsia is a pregnancy-associated disorder that manifests as a sudden increase in maternal blood pressure accompanied by proteinuria. Because the placenta is a key organ in preeclampsia, we used proteomic and lipidomic analyses to compare placentae from preeclamptic and gestational age matched control pregnancies. Fatty acid binding protein 4 (FABP4), enoyl-CoA dehydrogenase and delta-3,5-delta-2,4-dienoyl-CoA isomerase had altered abundance in preeclamptic placentae compared to controls. FABP4 placental protein and RNA and plasma levels were all increased in early-onset preeclampsia (prior to 28 weeks gestation) compared to controls (6-fold, 3.3-fold and 3.5-fold respectively). After 28 weeks, FABP4 protein in control placenta and plasma increased to the same concentrations as in preeclampsia. Total tetracosapentaenoic acid in preeclamptic placentae was decreased to 0.6 of control levels before 28 weeks. The data indicate a disruption of fatty acid transport and metabolism in the placenta in early onset preeclampsia that is reflected in the maternal plasma
Early onset preeclampsia is characterized by altered placental lipid metabolism and a premature increase in circulating FABP4
Methyl Jasmonate Responsive Proteins in <i>Brassica napus</i> Guard Cells Revealed by iTRAQ-Based Quantitative Proteomics
Stomata on leaf epidermis formed by pairs of guard cells
control CO<sub>2</sub> intake and water transpiration, and respond
to different environmental conditions. Stress-induced stomatal closure
is mediated via an intricate hormone network in guard cells. Although
methyl jasmonate (MeJA) has been intensively studied for its function
in plant defense, the molecular mechanisms underlying its function
in stomatal movement are not fully understood. Here we report the
effects of MeJA on <i>Brassica napus</i> stomatal movement
and H<sub>2</sub>O<sub>2</sub> production. Using the isobaric tags
for relative and absolute quantitation (iTRAQ) approach, we have identified
84 MeJA-responsive proteins in <i>B. napus</i> guard cells.
Most of the genes encoding these proteins contain jasmonate-responsive
elements in the promoters, indicating that they are potentially regulated
at the transcriptional level. Among the identified proteins, five
protein changes after MeJA treatment were validated using Western
blot analysis. The identification of the MeJA-responsive proteins
has revealed interesting molecular mechanisms underlying MeJA function
in guard cells, which include homeostasis of H<sub>2</sub>O<sub>2</sub> production and scavenging, signaling through calcium oscillation
and protein (de)phosphorylation, gene transcription, protein modification,
energy balance, osmoregulation, and cell shape modulation. The knowledge
of the MeJA-responsive proteins has improved our understanding of
MeJA signaling in stomatal movement, and it may be applied to crop
engineering for enhanced yield and stress tolerance
Methyl Jasmonate Responsive Proteins in <i>Brassica napus</i> Guard Cells Revealed by iTRAQ-Based Quantitative Proteomics
Stomata on leaf epidermis formed by pairs of guard cells
control CO<sub>2</sub> intake and water transpiration, and respond
to different environmental conditions. Stress-induced stomatal closure
is mediated via an intricate hormone network in guard cells. Although
methyl jasmonate (MeJA) has been intensively studied for its function
in plant defense, the molecular mechanisms underlying its function
in stomatal movement are not fully understood. Here we report the
effects of MeJA on <i>Brassica napus</i> stomatal movement
and H<sub>2</sub>O<sub>2</sub> production. Using the isobaric tags
for relative and absolute quantitation (iTRAQ) approach, we have identified
84 MeJA-responsive proteins in <i>B. napus</i> guard cells.
Most of the genes encoding these proteins contain jasmonate-responsive
elements in the promoters, indicating that they are potentially regulated
at the transcriptional level. Among the identified proteins, five
protein changes after MeJA treatment were validated using Western
blot analysis. The identification of the MeJA-responsive proteins
has revealed interesting molecular mechanisms underlying MeJA function
in guard cells, which include homeostasis of H<sub>2</sub>O<sub>2</sub> production and scavenging, signaling through calcium oscillation
and protein (de)phosphorylation, gene transcription, protein modification,
energy balance, osmoregulation, and cell shape modulation. The knowledge
of the MeJA-responsive proteins has improved our understanding of
MeJA signaling in stomatal movement, and it may be applied to crop
engineering for enhanced yield and stress tolerance
Methyl Jasmonate Responsive Proteins in <i>Brassica napus</i> Guard Cells Revealed by iTRAQ-Based Quantitative Proteomics
Stomata on leaf epidermis formed by pairs of guard cells
control CO<sub>2</sub> intake and water transpiration, and respond
to different environmental conditions. Stress-induced stomatal closure
is mediated via an intricate hormone network in guard cells. Although
methyl jasmonate (MeJA) has been intensively studied for its function
in plant defense, the molecular mechanisms underlying its function
in stomatal movement are not fully understood. Here we report the
effects of MeJA on <i>Brassica napus</i> stomatal movement
and H<sub>2</sub>O<sub>2</sub> production. Using the isobaric tags
for relative and absolute quantitation (iTRAQ) approach, we have identified
84 MeJA-responsive proteins in <i>B. napus</i> guard cells.
Most of the genes encoding these proteins contain jasmonate-responsive
elements in the promoters, indicating that they are potentially regulated
at the transcriptional level. Among the identified proteins, five
protein changes after MeJA treatment were validated using Western
blot analysis. The identification of the MeJA-responsive proteins
has revealed interesting molecular mechanisms underlying MeJA function
in guard cells, which include homeostasis of H<sub>2</sub>O<sub>2</sub> production and scavenging, signaling through calcium oscillation
and protein (de)phosphorylation, gene transcription, protein modification,
energy balance, osmoregulation, and cell shape modulation. The knowledge
of the MeJA-responsive proteins has improved our understanding of
MeJA signaling in stomatal movement, and it may be applied to crop
engineering for enhanced yield and stress tolerance
Methyl Jasmonate Responsive Proteins in <i>Brassica napus</i> Guard Cells Revealed by iTRAQ-Based Quantitative Proteomics
Stomata on leaf epidermis formed by pairs of guard cells
control CO<sub>2</sub> intake and water transpiration, and respond
to different environmental conditions. Stress-induced stomatal closure
is mediated via an intricate hormone network in guard cells. Although
methyl jasmonate (MeJA) has been intensively studied for its function
in plant defense, the molecular mechanisms underlying its function
in stomatal movement are not fully understood. Here we report the
effects of MeJA on <i>Brassica napus</i> stomatal movement
and H<sub>2</sub>O<sub>2</sub> production. Using the isobaric tags
for relative and absolute quantitation (iTRAQ) approach, we have identified
84 MeJA-responsive proteins in <i>B. napus</i> guard cells.
Most of the genes encoding these proteins contain jasmonate-responsive
elements in the promoters, indicating that they are potentially regulated
at the transcriptional level. Among the identified proteins, five
protein changes after MeJA treatment were validated using Western
blot analysis. The identification of the MeJA-responsive proteins
has revealed interesting molecular mechanisms underlying MeJA function
in guard cells, which include homeostasis of H<sub>2</sub>O<sub>2</sub> production and scavenging, signaling through calcium oscillation
and protein (de)phosphorylation, gene transcription, protein modification,
energy balance, osmoregulation, and cell shape modulation. The knowledge
of the MeJA-responsive proteins has improved our understanding of
MeJA signaling in stomatal movement, and it may be applied to crop
engineering for enhanced yield and stress tolerance