11 research outputs found
Fermentatative production of itaconic acid by Aspergillus terreus using Jatropha seed cake
Fermentation process for the production of itaconic acid was carried out using jatropha seed cake. Itaconic acid is commercially produced by the cultivation of Aspergillus terreus with molasses. Jatropha seed cake is one of the best carbon sources among various carbohydrates, because it is pure, inexpensive and available in a mass supply. The reaction was carried out at various temperatures, agitations and pH. The samples were collected at 24 h time intervals. Itaconic acid concentration wasmeasured by the rapid spectroscopic method. Jatropha seed cake shows maximum yield of 24.45g/lafter 120 h
Valorizing the 'Irulas' traditional knowledge of medicinal plants in the Kodiakkarai Reserve Forest, India
A mounting body of critical research is raising the credibility of Traditional Knowledge (TK) in scientific studies. These studies have gained credibility because their claims are supported by methods that are repeatable and provide data for quantitative analyses that can be used to assess confidence in the results. The theoretical importance of our study is to test consensus (reliability/replicable) of TK within one ancient culture; the Irulas of the Kodiakkarai Reserve Forest (KRF), India. We calculated relative frequency (RF) and consensus factor (Fic) of TK from 120 Irulas informants knowledgeable of medicinal plants. Our research indicates a high consensus of the Irulas TK concerning medicinal plants. The Irulas revealed a diversity of plants that have medicinal and nutritional utility in their culture and specific ethnotaxa used to treat a variety of illnesses and promote general good health in their communities. Throughout history aboriginal people have been the custodians of bio-diversity and have sustained healthy life-styles in an environmentally sustainable manner. However this knowledge has not been transferred to modern society. We suggest this may be due to the asymmetry between scientific and TK, which demands a new approach that considers the assemblage of TK and scientific knowledge. A greater understanding of TK is beginning to emerge based on our research with both the Irulas and Malasars; they believe that a healthy lifestyle is founded on a healthy environment. These aboriginal groups chose to share this knowledge with society-at-large in order to promote a global lifestyle of health and environmental sustainability
Chandana Lakshmi et al. ISSN: 22312781 925 INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY OPTIMIZATION OF PHENOL DEGRADATION FROM Pseudomonas aeruginosa (NCIM 2074) USING RESPONSE SURFACE METHODOLOGY
Association of The Common CYP1A1*2C Variant (Ile462Val Polymorphism) with Chronic Myeloid Leukemia (CML) in Patients Undergoing Imatinib Therapy
Objective: Cytochrome P450 is one of the major drug metabolizing enzyme families and
its role in metabolism of cancer drugs cannot be less emphasized. The association between
single nucleotide polymorphisms (SNPs) in CYP1A1 and pathogenesis of chronic
myeloid leukemia (CML) has been investigated in several studies, but the results observed
vary based on varied risk factors. The objective of this study was to investigate the risk
factors associated with the CYP1A1*2C [rs1048943: A>G] polymorphism in CML patients
and its role in therapeutic response to imatinib mesylate (IM) affecting clinico-pathological
parameters, in the Indian population.
Materials and Methods: In this case-control study, CYP1A1*2C was analysed in CML
patients. After obtaining approval from the Ethics Committee of oncology hospital, we
collected blood samples from 132 CML patients and 140 matched controls. Genomic
DNA was extracted and all the samples were analysed for the presence of the
CYP1A1*2C polymorphism using allele-specific polymerase chain reaction, and we
examined the relationship of genotypes with risk factors such as gender, age, phase
of the disease and other clinical parameters.
Results: We observed a significant difference in the frequency distribution of CYP1A1*2C
genotypes AA (38 vs. 16%, P=0.0001), AG (57 vs. 78%, P=0.0002) and GG (5 vs. 6%,
P=0.6635) between patients and controls. In terms of response to IM therapy, significant
variation was observed in the frequencies of AA vs AG in major (33 vs 67%) and poor (62
vs 31%) hematological responders, and AA vs AG in major (34 vs. 65%) and poor (78 vs.
22%) cytogenetic responders. However, the patients with the GG homozygous genotype
did not show any significant therapeutic outcome.
Conclusion: The higher frequency of AG in controls indicates that AG may play a protective
role against developing CML. We also found that patients with the AG genotype showed
favorable treatment response towards imatinib therapy, indicating that this polymorphism
could serve as a good therapeutic marker in predicting response to such therapy