Expressão e distribuição da conexina 32 em fígados de ratos com fibrose induzida experimentalmente

The connexin 32 (Cx32) is a protein that forms the channels that promote the gap junction intercellular communication (GJIC) in the liver, allowing the diffusion of small molecules through cytosol from cell-to-cell. Hepatic fibrosis is characterized by a disruption of normal tissue architeture by cellular lesions, and may alter the GJIC. This work aimed to study the expression and distribution of Cx32 in liver fibrosis induced by the oral administration of dimethylnitrosamine in female Wistar rats. The necropsy of the rats was carried out after five weeks of drug administration. They presented a hepatic fibrosis state. Sections from livers with fibrosis and from control livers were submitted to immunohistochemical, Real Time-PCR and Western-Blot analysis to Cx32. In fibrotic livers the Cxs were diffusely scattered in the cytoplasm, contrasting with the control livers, where the Cx32 formed junction plaques at the cell membrane. Also it was found a decrease in the gene expression of Cx32 without reduction in the protein quantity when compared with controls. These results suggest that there the mechanism of intercellular communication between hepatocytes was reduced by the fibrotic process, which may predispose to the occurrence of a neoplastic process, taken in account that connexins are considered tumor suppressing genes.A conexina 32 (Cx32) é uma proteína que constitui os canais que promovem as comunicações intercelulares via junções comunicantes (CIJC) no fígado, permitindo difusão de pequenas moléculas citoplasmáticas de uma célula à outra. A fibrose hepática caracteriza-se pela alteração da arquitetura normal do fígado e podem alterar as CIJCs. O objetivo deste trabalho foi estudar a expressão e distribuição de Cx32 na fibrose hepática. O objetivo do presente trabalho foi estudar a expressão e distribuição da Cx32 em fígados com fibrose induzida pela administração oral de dimetilnitrosamina em fêmeas de ratos Wistar. A necropsia foi realizada após cinco semanas da última administração da droga e observou-se um quadro de fibrose hepática. Amostras dos fígados com fibrose e de animais controle foram submetidas à análise imunoistoquímica, por Real Time-PCR e por Western-Blot verificando-se a presença de Cx32 difusa e dispersa no citoplasma dos fígados com fibrose. No grupo controle a Cx32 localizou-se na membrana citoplasmática com a formação de placas juncionais. O fígado com fibrose também revelou diminuição da expressão gênica de Cx32, embora sem a redução da quantidade do produto protéico, quando comparado ao grupo controle. Estes resultados sugerem que o mecanismo de comunicação intercelular entre os hepatócitos reduziu-se durante o processo fibrótico, o que pode predispor a ocorrência de processos neoplásicos, uma vez que as conexinas são consideradas genes supressores de tumores.FAPESPCNP

Chemical carcinogenesis by 7,12-dimethylbenzanthracene in balb/c mice

FAPES

A reevaluation of the late quaternary sedimentation in todos os Santos Bay (BA), Brazil

Todos os Santos Bay is a large (<img ALIGN="BOTTOM" BORDER="0" SRC="http:/img/fbpe/aabc/v72n4/0052img1.gif" ALT="$approx$"> 1000 km²), structurally controlled tidal bay in northeast Brazil. Three main drainage basins debouch into the bay, providing a mean freshwater discharge of 200 m³/s (prior to 1985), or less than 1% of the spring tidal discharge through the bay mouth. Based on the result of several sedimentological studies performed in the 1970's, five surface sedimentary facies were identified inside the bay, namely i) transgressive siliciclastic marine sand facies; ii) transgressive bay sand-mud facies; iii) a transgressive carbonate marine sand facies; iv) regressive bay-mud facies, and v) regressive fluvial sand facies. The spatial distribution of these facies would follow, somewhat closely, the hydrodynamic-energy distribution inside the bay. Seismic profiles along the bay bottom indicate the existence of several paleochannels, 5-10 m deep, blanketed at least by three different sedimentary units. The topmost sedimentary unit, 5-20 m thick, appears to be associated with the regressive bay-mud facies, and assuming that it was laid down within the last 5000 years, sedimentation rates for the central and northeastern part of the bay would average at 2,4 mm/y