Genotipske i alelske frekvencije polimorfizama u ABCG2, PPARGC1A i OLR1 genima kod domaćih rasa goveda u Turskoj

This study was carried out to determine polymorphisms of four genes in South Anatolian Red (SAR) and East Anatolian Red (EAR) indigenous cattle breeds in Turkey. Single nucleotide polymorphisms (SNPs) mtor gamma coactivator 1 alpha (PPARGC1A) gene and g.8232C>A in oxidized low-density lipoprotein receptor 1 (OLR1) gene. The frequency of the ancestral allele A of the ABCG2 gene Y581S polymorphism was found to be very high (SAR: 0.63; EAR: 0.64) in both cattle breeds. The CC genotypes of PPARGC1A gene c.1892T>C (SAR: 0.65; EAR: 0.80) and OLR1 gene g.8232C>A polymorphisms (SAR: 0.82; EAR: 0.86), which are associated with high milk fat percentage, had higher frequencies than those of the other genotypes. In conclusion, onitored in this study are Y581S in ATP binding cassette sub family G member 2 (ABCG2) gene, c.1892T>C and c.3359A>C in peroxisome proliferator-activated recepwe might suggest that the allele distribution of the ABCG2 gene Y581S polymorphism can be the evidence indicating autosomal gene flow from zebu cattle to SAR and EAR cattle breeds.Studija je obavljena u cilju određivanja polimorfi zma četiri gena kod južne anadolijske crvene (SAR) i istočne anadolijske crvene (EAR) domaće rase goveda u Turskoj. Pojedinačni nukleotidni polimorfi zmi (SNPs) koji su posmatrani u ovoj studiji su Y581S u ATP vezujućoj kaseti podfamilije G člana 2 (ABCG2) gena, c.1892T>C i c.3359A>C u PPARGC1A genu i g.8232C>A u genu za oksidisani lipoproteinski receptor male gustine (OLR1). Ustanovljeno je da je učestalost polimorfi zma alela A predaka ABCG2 gena Y581S veoma visoka (SAR: 0.63; EAR:0.64) i to kod obe rase goveda. Učestalost polimorfi zama CC genotipova PPARGC1A gena c. 1982T>C (SAR: 0.65; EAR: 0.80) i OLR1 gena g.8232C>A (SAR: 0.82; EAR: 0.86) koji su povezani sa visokim procentom masti u mleku, bile su veće u poređenju sa drugim genotipovima. U zaključku može da se sugeriše da distribucija polimorfi zma alela ABCG2 gena Y581S može da bude pokazatelj putanje autozomnog gena od zebu govečeta do SAR i EAR rasa goveda

Effect of reduced energy diet on some blood biochemical indicators in the late pregnant ewes

The objective of the present study was to investigate effects of feeding pregnant ewes with a reduced-energy diet during the late gestation period by monitoring changes in blood levels of some biochemical parameters. A total of 30 5-6-year-old Chios ewes (20 pregnant, 10 non-pregnant) were used. On day 105 of their gestation the pregnant ewes were divided into 2 treatment groups of 10 animals in each: an energy restricted group (ER) and normal energy group (NE). The ewes that were not pregnant were assigned as a group of non-pregnant ewes (NP). Blood samples were taken from the ewes of all groups on days 120, 127, 134, 141, and 148 of gestation and analysed in order to determine levels of total protein, albumin, globulin, albumin/globulin (A/G) ratio, glucose, and total lipids. The results indicated that the levels of total proteins and globulin were found to be lower in the last 4 weeks of gestation in ER and NE groups as compared to those in the NP group. Levels of total protein and globulin in NE and ER groups persistently decreased between days 120 and 148 (P < 0.05). During the last 4 weeks of gestation glucose levels of pregnant ewes were found to be significantly lower (P < 0.05) as compared to those of NP ewes. No difference was discovered between NE and ER groups in the levels of total protein, albumin, globulin, glucose and A/G rations. Levels of total lipids constantly increased in the last 5 weeks of gestation. The highest increase in total lipids was seen in the ER group ewes (P < 0.05). These findings indicate that feeding with a reduced energy diet may not adversely affect maternal immunity and transfer of Igs to colostrum. However, significant increases in total lipids in the ER group indicate that increased energy needed for fast fetal growth was met by the mobilisation of reserve fats