13 research outputs found
Genomic Analysis of the Hydrocarbon-Producing, Cellulolytic, Endophytic Fungus Ascocoryne sarcoides
The microbial conversion of solid cellulosic biomass to liquid biofuels may provide a renewable energy source for transportation fuels. Endophytes represent a promising group of organisms, as they are a mostly untapped reservoir of metabolic diversity. They are often able to degrade cellulose, and they can produce an extraordinary diversity of metabolites. The filamentous fungal endophyte Ascocoryne sarcoides was shown to produce potential-biofuel metabolites when grown on a cellulose-based medium; however, the genetic pathways needed for this production are unknown and the lack of genetic tools makes traditional reverse genetics difficult. We present the genomic characterization of A. sarcoides and use transcriptomic and metabolomic data to describe the genes involved in cellulose degradation and to provide hypotheses for the biofuel production pathways. In total, almost 80 biosynthetic clusters were identified, including several previously found only in plants. Additionally, many transcriptionally active regions outside of genes showed condition-specific expression, offering more evidence for the role of long non-coding RNA in gene regulation. This is one of the highest quality fungal genomes and, to our knowledge, the only thoroughly annotated and transcriptionally profiled fungal endophyte genome currently available. The analyses and datasets contribute to the study of cellulose degradation and biofuel production and provide the genomic foundation for the study of a model endophyte system
The effect of intellectual ability on functional activation in a neurodevelopmental disorder: preliminary evidence from multiple fMRI studies in Williams syndrome
An Automated Method for Chemical Composition Analysis of Lubricant Base Oils by Using Atmospheric Pressure Chemical Ionization Mass Spectrometry
Screening Brazilian Automotive Gasoline Quality through Quantification of Saturated Hydrocarbons and Anhydrous Ethanol by Gas Chromatography and Exploratory Data Analysis
Preparation of mesoporous silica supported sulfonic acid and evaluation of the catalyst in esterification reactions
E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion
Heterotrimeric kinesin-II is necessary and sufficient to promote different stepwise assembly of morphologically distinct bipartite cilia in Drosophila antenna
Structurally distinct sensory cilia in the fly antenna grow in different stepwise patterns. The heterotrimeric kinesin-II is essential for growth at all stages, and it is also required for tubulin entry into the cilia from the beginning. This report establishes a primary function of the motor in the bipartite cilia assembly in Drosophila