35 research outputs found
Production of a Fusion Protein Consisting of the Enterotoxigenic Escherichia coli Heat Labile Toxin B Subunit and a Tuberculosis Antigen in Arabidopsis thaliana.
Development and function of membrane systems in plant tissue. Annual progress report, 15 July 1974--14 July 1975. [Ion transport in corn roots]
Non
Herbicide-resistant crops: yield penalties and weed thresholds for oilseed rape (Brassica napus L.)
Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato
Epitopes often require co-delivery with an adjuvant or targeting protein to enable recognition by the immune system. This paper reports the ability of transgenic tomato plants to express a fusion protein consisting of the B subunit of the Escherichia coli heat-labile enterotoxin (LTB) and an immunocontraceptive epitope. The fusion protein was found to assemble into pentamers, as evidenced by its ability to bind to gangliosides, and had an average expression level of 37.8 µg g -1 in freeze-dried transgenic tissues. Processing of selected transgenic fruit resulted in a 16-fold increase in concentration of the antigen with minimal loss in detectable antigen.
The species-specific nature of this epitope was shown by the inability of antibodies raised against non-target species to detect the LTB fusion protein. The immunocontraceptive ability of this vaccine will be tested in future pilot mice studies