The effect of erythropoietin on ?-glutamyltransferase during ischemia reperfusion injury in rats

The aim of this experimental study was to examine the effect of erythropoietin on rat model and particularly in an ischemia reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean ?-glutamyltransferase (?GT) levels. Materials and methods: 40 rats of mean weight 247.7 g were used in the study. ?GT levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results were that Epo administration non-significantly decreased the ?GT levels by 12.70% +13.11% results of paired t-test (p= 0.3541). Reperfusion time kept non-significantly increased the ?GT levels by 6.35%+13.24% (P=0.6264). However, erythropoietin administration and reperfusion time together produced a non-significant combined effect in keeping decreased the ?GT levels by 4.62%+7.97% (P= 0.5534). Conclusions are that erythropoietin administration interacted or not with reperfusion time have non significant short term decreasing effects on ?GT levels

Akutan učinak antioksidantnog lijeka U-74389G kod srednjih razina crvenih krvnih tjelešaca za vrijeme hipoksije i ponovljene oksigenacije kod štakora

Aim: The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G”, on rat model and particularly in a hypoxia – reoxygenation protocol. The beneficial effect or non-effectiveness of that molecule was studied hematologically using blood mean corpuscular hemoglobin (MCH) levels. Methods: 40 rats of mean weight 231.875 gr were used in the study. MCH levels were measured 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D) with the administration of drug U-74389G in groups C and D. Results: The results were that U-74389G administration significantly increased the MCH levels by 2.40% ± 0.57% (p = 0.0001). Reoxygenation time non-significantly decreased the MCH levels by 0.48% ± 0.69 (p = 0.4103). However, U-74389G administration and reoxygenation time together significantly increased the MCH levels by 1.33% ± 0.36% (p = 0.0005). Conclusion: The results of this study indicate that U-74389G administration either alone or interacted with reoxygenation time has significant increasing short – term effects on the pathophysiology recovery of MCH values.Cilj: Cilj ove eksperimentalne studije je ispitatiučinakantioksidantnog lijeka “U-74389G”, na model štakora, a posebno u protokolu hipoksije i ponovljene oksigenacije. Blagotvoran učinak ili neučinkovitost navedene molekule ispitani su hematološki korištenjem srednjih razina crvenih krvnih tjelešaca (MCH). Metode: U studiji je korišteno 40 štakora srednje težine od 231,875 g. MCH razine izmjerene su 60 min nakon reperfuzije (skupine A i C) i 120 min nakon reperfuzije (skupine B i D) davanjem lijeka U-74389G skupinama C i D. Rezultati: Davanje lijeka U-74389G značajno je povećalo razine MCH za 2,40% ± 0,57% (p = 0,0001). Ponovljena oksigenacija smanjila je beznačajno razine MCH za 0,48% ± 0,69 (p = 0,4103). Davanje U-74389G tijekom ponovljene oksigenacije međutim, značajno je povećalo MCH razine za 1,33% ± 0,36% (p = 0,0005). Zaključak: Rezultati ove studije pokazuju da zasebno davanje U-74389G ili u interakciji s ponovljenom oksigenacijom, značajno povećava kratkotrajne učinke na oporavak patofiziološke razine MCH

Restenosis is not associated with stent length in a pig model of coronary stent implantation

Background: The aim of this study was to determine if stent length is by itself a risk factor for intimal proliferation and restenosis. Long lesions represent an independent risk factor for restenosis after coronary stent implantation. A longer stented segment might result in a higher probability of restenosis. Methods: Twenty-two 7-month-old male farm pigs underwent implantation of two steel stents, one short (8 mm length) and one long (16 mm length), in the right coronary artery. The pigs were sacrificed 28 days after stent implantation and histomorphometric analysis of the coronary arteries was performed for neointimal area proliferation and area stenosis evaluation. Results: Seventeen short stents and 19 long stents were finally implanted. There were no differences in neointimal proliferation (1.84 ± 0.64 mm2 vs. 1.81 ± 0.94 mm2, p = 0.84), area stenosis (40 ± 9% vs. 41 ± 19%, p = 0.86) and lumen area (2.96 ± 1.30 mm2 vs. 2.51 ± ± 1.18 mm2, p = 0.21) between the short stent group and the long stent group, respectively. Conclusions: These data suggest that stent length by itself does not influence restenosis extent in the porcine model

The trends of the antioxidant drug “U-74389G” on potassium levels during hypoxia reoxygenation injury in rats

Background: This experimental study examined the trends of the antioxidant drug “U-74389G”, on a rat model and particularly in a hypoxia – reoxygenation (HR) protocol. The trends of that molecule were studied biochemically using blood mean potassium levels. Methods: 40 rats of mean weight 231.875 g were used in the study. Potassium (K+) levels were measured at 60 min of reoxygenation (groups A and C) and at 120 min of reoxygenation (groups B and D) with administration of the drug U-74389G in groups C and D. Results: U-74389G administration non significantly decreased the K+ levels by 2.14%+5.06% (p= 0.6730). Reoxygenation time non-significantly increased the K+ levels by 8.66%+4.85% (p= 0.0934). However, U-74389G administration and reoxygenation time together non-significantly increased the K+ levels by 2.07%+3.03% (P= 0.4853). Conclusions: U-74389G administration, reoxygenation time and their interaction have miscellaneous non significant short – term trends on potassium levels. Perhaps, a longer study time or a higher drug dose may reveal clearer and significant effects

The effect of erythropoietin on chloride levels during hypoxia reoxygenation injury in rats

Objective. This experimental study examined the effect of erythropoietin (Epo) in a rat model and particularly in a hypoxiareoxygenation (HR) protocol. The effect of that molecule was studied biochemically using blood mean chloride (Cl) levels. Materials and methods. 40 rats of mean weight 247.7 g were used in the study. Cl levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Erythropoietin was administered only in groups C and D. Results. Epo administration non-significantly decreased Cl levels by 1.07%+0.91% (p=0.2635). Reoxygenation time nonsignificantly decreased Cl levels by 0.68%+0.92% (P= 0.4457). However, erythropoietin administration and reoxygenation time together produced a non-significant combined effect in decreasing Cl levels by 0.74%+0.54% (P= 0.1701). Conclusions. Epo administration, reoxygenation time and their interaction have non-significant, short-term, decreasing effects on Cl levels

The effect of erythropoietin on endosalpingeal karyorrhexis during ischemia reperfusion injury in rats

Introduction. The aim of this experiment was to study the effects of the antioxidant drug “U-74389G” on rat model, particularly in ischemia reperfusion protocol. The beneficial or other effects of that molecule were studied estimating the mean endosalpingeal karyorrhexis (EK) lesions. Material and methods. 40 rats were used of mean weight 247.7 g. EK was evaluated 60 min after reperfusion for groups A and C and 120 min after reperfusion for groups B and D. Groups A and B without the drug but C and D with erythropoietin administration. Results. Results were that erythropoietin administration kept non-significantly increased the EK scores by 0.1 (–0.0393284–0.2393284) (p = 0.1544). This finding was in accordance with the results of Wilcoxon signed-rank test (p = 0.1573). Reperfusion time non-significantly decreased the EK scores by 0.1 (–0.2393284–0.0393284) (p = 0.1544), approximately in accordance with the increased EK score by 0.1 (–0.2440518–0.0440518) of Wilcoxon signed-rank test (p = 0.1573). However, Epo administration and reperfusion time together kept non-significantly increased the EK scores by 0.0181818 (–0.0679319–0.1042955) (p = 0.6715). Conclusions. Results of this study indicate that Epo administration kept non-significantly increased the EK scores. Perhaps, a longer study time than 2 hours may provide more significant effects.Due to impossible pasting, you are kindly asked to find the abstract in main documen

A conceptual framework for evaluating national organ donation and transplantation programs

Conceptual frameworks are valuable resources that can be used to guide the planning, evaluation, and development of healthcare services. However, there are currently no comprehensive frameworks focused on organ donation and transplantation that identify the critical factors underlying a successful national program. To address this knowledge gap, we developed a conceptual framework that takes into account all major domains of influence, including political and societal aspects as well as clinical implementation. The framework was initially constructed based on a targeted review of the relevant medical literature. Feedback provided by a panel of international experts was incorporated into the framework via an iterative process. The final framework features 16 essential domains that are critical for initiating and maintaining a successful program and improving the health of patients with organ failure. Of particular note, these domains are subject to three overarching health system principles: responsiveness, efficiency, and equity. This framework represents a first attempt to develop a whole-system view of the various factors that contribute to the success of a national program. These findings provide a useful tool that can be adapted to any jurisdiction and used to plan, evaluate, and improve organ donation and transplantation programs

The effect of erythropoietin on albumins levels during hypoxia reoxygenation injury in rats

The aim of this experimental study was to examine the effect of erythropoietin on rat model and particularly in an hypoxia reoxygenation (HR) protocol. The effect of that molecule was studied biochemically using blood mean albumins levels.Materials and methods: 40 rats of mean weight 247.7 g were used in the study. Albumins levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Erythropoietin was administered only in groups C and D.Results were that Epo administration significantly decreased the albumins levels by 9.28%+3.20% (p=0.0054). Reperfusion time non-significantly increased the albumins levels by 3.09%+3.52%  (p= 0.3405). However, erythropoietin administration and reperfusion time together produced a significant combined effect in keeping decreased the albumins levels by 5.37%+2.73% (p= 0.0072).Conclusions are that erythropoietin administration whether it interacted or not with reoxygenation time, has significant decreasing effects on albumins levels in a short-term context of 2 hours