9 research outputs found
Measurements of Protein Structure and Noncovalent Interactions by Time-of-Flight Mass Spectrometry with Orthogonal Ion Injection
No full text
Critical Moments in Time: Reflections on the Development of Orthogonal Acceleration Time-of-Flight Mass Spectrometry
No full textMonitoring macromolecular complexes involved in the chaperonin-assisted protein folding cycle by mass spectrometry
No full textMass Spectrometry
No full textInternational audienceFor twenty years or so now, mass spectrometry has been used to get exact measurements of the mass of biological molecules such as proteins, nucleic acids,oligosaccharides, and so on. Over the past ten years, this technology has followed the trend toward miniaturisation and the samples required can be much smaller. In particular, the nanoelectrospray source (online or by needle) allow one to work at flow rates of a few tens of nanolitres/min. There are many applications, both in the field of proteomics and in the analysis of protein structure, dynamics, and interactions. Combining this source with nanoHPLC, complex mixtures only available in small quantities can be separated and analysed online. There are also some advantages over conventional HPLC, despite a set of constraints related to the small dimensions and low flow rates. Combining capillary electrophoresis with the electrospray source also gives useful results, with its own set of advantages and constraints. Finally, developments are currently underway to combine this source with chips, providing a means of separation and analysis online
