Curso sobre o SiBCTI: embasamento teórico e prático com enfoque na região Semiárida.

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Enzymatic regulation of glycogenolysis in a subarctic population of the wood frog: implications for extreme freeze tolerance

The wood frog, Rana sylvatica, from Interior Alaska survives freezing at –16°C, a temperature 10–13°C below that tolerated by its southern conspecifics. We investigated the hepatic freezing response in this northern phenotype to determine if its profound freeze tolerance is associated with an enhanced glucosic cryoprotectant system. Alaskan frogs had a larger liver glycogen reserve that was mobilized faster during early freezing as compared to conspecifics from a cool-temperate region (southern Ohio, USA). In Alaskan frogs the rapid glucose production in the first hours of freezing was associated with a 7-fold increase in glycogen phosphorylase activity above unfrozen frog levels, and the activity of this enzyme was higher than that of frozen Ohioan frogs. Freezing of Ohioan frogs induced a more modest (4-fold) increase in glycogen phosphorylase activity above unfrozen frog values. Relative to the Ohioan frogs, Alaskan frogs maintained a higher total protein kinase A activity throughout an experimental freezing/thawing time course, and this may have potentiated glycogenolysis during early freezing. We found populational variation in the activity and protein level of protein kinase A which suggested that the Alaskan population had a more efficient form of this enzyme. Alaskan frogs modulated their glycogenolytic response by decreasing the activity of glycogen phosphorylase after cryoprotectant mobilization was well under way, thereby conserving their hepatic glycogen reserve. Ohioan frogs, however, sustained high glycogen phosphorylase activity until early thawing and consumed nearly all their liver glycogen. These unique hepatic responses of Alaskan R. sylvatica likely contribute to this phenotype’s exceptional freeze tolerance, which is necessary for their survival in a subarctic climate

Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica

We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of tolerance for spring frogs was between −2.5°C and −5°C. Spring frogs had comparatively low levels of the urea in blood plasma, liver, heart, brain, and skeletal muscle, as well as a smaller hepatic reserve of glycogen, which is converted to glucose after freezing begins. Consequently, following freezing (−2.5°C, 48 h) tissue concentrations of these cryoprotective osmolytes were 44–88% lower than those measured in winter frogs. Spring frogs formed much more ice and incurred extensive cryohemolysis and lactate accrual, indicating that they had suffered marked cell damage and hypoxic stress during freezing. Multiple, interactive stresses, in addition to diminished cryoprotectant levels, contribute to the reduced capacity for freeze tolerance in posthibernal frogs

Aplicação de técnicas de classificação semissupervisionada para análise de séries multitemporais de imagens de satélite.

Este trabalho apresenta uma comparação de dois algoritmos de classificação semissupervisionada utilizados para auxiliar na identificação de áreas de cultivo de cana-de-açúcar, uma importante commoditie brasileira. As técnicas foram incorporadas ao software SatImagExplorer, que foi desenvolvido para auxiliar na extração de séries temporais de imagens de satélite (CHINO; ROMANI; TRAINA, 2010). Os resultados indicam que ambas as técnicas apresentaram resultados satisfatórios para classificação de diferentes classes usando séries de imagens de baixa resolução espacial.SBSR 2013

Water availability in a Planosol under integrated crop-livestock-forestry system in the agreste region of Paraiba, Brazil.

Os sistemas integrados de produção agropecuária, envolvendo lavoura, pecuária e floresta (ILPF) são estratégias que promovem a qualidade do solo. Objetivou-se com esse trabalho avaliar a influência do sistema de integração Lavoura-Pecuária-Floresta na disponibilidade de água em um Planossolo na mesorregião do Agreste da Paraíba. O experimento foi conduzido na estação experimental da Empresa de Pesquisa Agropecuária da Paraíba (EMEPA), em Alagoinha (PB). O solo é um Planossolo Háplico, horizonte A moderado. Em junho de 2015, foram implantados os seguintes consórcios: I. Gliricídia (Gliricidia sepium (Jacq.) Steud.) + Brachiaria decumbens; II. Sabiá (Mimosa caesalpiniifolia) + Brachiaria decumbens; III. Ipê amarelo (Tabebuia alba (chamiso) sandwith) + Brachiaria decumbens; IV. Milho (Zea mays L.) + Brachiaria decumbens; V. Brachiaria decumbens. Utilizou-se o delineamento de blocos casualizados (DBC), com cinco tratamentos. As variáveis analisadas foram: capacidade de campo - tetaCC, ponto de murcha permanente - tetaPMP, água disponível - tetaAD, granulometria, grau de floculação (GF), porosidade (total - alfa, macro - Ma e micro - Mi), condutividade hidráulica saturada - Kteta, resistência a penetração - Rp, índice de estabilidade de agregados - IEA. De acordo com os resultados observa-se que a disponibilidade de água na integração lavoura-pecuária-floresta implantada no agreste paraibano não apresentou variação significativa após um período de três anos. A integração, após o período de três anos, ainda não promoveu melhoria física significativa dos atributos do Planossolo em estudo

Produção de massa seca de capim papuã (Brachiaria plantaginea) submetido à adubação nitrogenada com ou sem irrigação em integração lavoura-pecuária.

Este trabalho objetivou quantificar a resposta de níveis de nitrogênio e de irrigação, com ou sem adubação de base, sobre a produção de massa seca do capim papuã

Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica

We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of tolerance for spring frogs was between −2.5°C and −5°C. Spring frogs had comparatively low levels of the urea in blood plasma, liver, heart, brain, and skeletal muscle, as well as a smaller hepatic reserve of glycogen, which is converted to glucose after freezing begins. Consequently, following freezing (−2.5°C, 48 h) tissue concentrations of these cryoprotective osmolytes were 44–88% lower than those measured in winter frogs. Spring frogs formed much more ice and incurred extensive cryohemolysis and lactate accrual, indicating that they had suffered marked cell damage and hypoxic stress during freezing. Multiple, interactive stresses, in addition to diminished cryoprotectant levels, contribute to the reduced capacity for freeze tolerance in posthibernal frogs