Potent Protective Effect Of &#945 -Tocopherol And Fish Oil On In Vivo Paraquat Induced Oxidative Damage In Rats

The potential protective role of &#945-tocopherol and fish oil against oxidative damage induced by paraquat were investigated. Forty male albino rats with average body weight of 100-120 gm were housed in 8 groups of 5 rats each. The first group served as control and injected with saline, group 2 was injected with a single dose of paraquat (10 mg/kg, intraperittoneally) for 24 h prior to decapitation (P), group 3 was administered orally with vitamin E (100 mg/kg) five times a week (E), group 4 was administered orally with fish oil (20 mg/kg) five times a week (FO); group 5 received FO+E, groups 6, 7 and 8 were administrated with P+E, P+FO and P+E+FO respectively. The content of microsomal proteins, drug metabolizing enzymes and thiobarbituric acid reactive substances (TBARS) were determined in liver microsome after treatment. Vitamin E together with fish oil significantly decreased the content of cytochrome b5 (