tRNA structural and functional changes induced by oxidative stress

Oxidatively damaged biomolecules impair cellular functions and contribute to the pathology of a variety of diseases. RNA is also attacked by reactive oxygen species, and oxidized RNA is increasingly recognized as an important contributor to neurodegenerative complications in humans. Recently, evidence has accumulated supporting the notion that tRNA is involved in cellular responses to various stress conditions. This review focuses on the intriguing consequences of oxidative modification of tRNA at the structural and functional level

Hydrogels: An Effective Tool to Improve Nitrogen Use Efficiency in Crops

Hydrogels are hydrophilic polymeric materials with three-dimensional(3D) network structures physic and/or chemically crosslinked. They can absorb large amounts of water or aqueous solutions for a short period of time. When the hydrogel is loaded with a drug and meets an aqueous medium, the water penetrates the system and dissolves the drug. To improve the nitrogen use efficiency by plants, controlled release fertilizers are developed with hydrogels as matrices. In particular, biodegradable polymers such as starch, xanthan, chitosan, cellulose derivatives, pectin and their mixtures have been essayed to avoid residues in soils. In addition, the superabsorbent capacity of the hydrogels improves the retention of fertilizers in the soil, reduces water losses through evaporation and decreases the frequency of irrigation.In this chapter, the behavior of different hydrogels to improve the availability of nitrogen by the plants during their crop cycle will be reviewed.Fil: Melaj, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnología en Polímeros y Nanotecnología. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnología en Polímeros y Nanotecnología; ArgentinaFil: Giménez, Rocío Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnología en Polímeros y Nanotecnología. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnología en Polímeros y Nanotecnología; Argentin

hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1

mRNA structure is important for post-transcriptional regulation, largely because it affects binding of trans-acting factors(1). However, little is known about the in vivo structure of full-length mRNAs. Here we present hiCLIP, a high-throughput technique to identify RNA secondary structures interacting with RNA-binding proteins (RBPs) in vivo. Using this technique to investigate RNA structures bound by Staufen 1 (STAU1), we uncover a dominance of intra-molecular RNA duplexes, a depletion of duplexes from coding regions of highly translated mRNAs, an unforeseen prevalence of long-range duplexes in 3′ untranslated regions (UTRs), and a decreased incidence of SNPs in duplex-forming regions. We also discover a duplex spanning 858nts in the 3′ UTR of the X-box binding Protein 1 (XBP1) mRNA that regulates its cytoplasmic splicing and stability. Our study reveals the fundamental role of mRNA secondary structures in gene regulation and introduces hiCLIP as a widely applicable method for discovering novel, especially long-range, RNA duplexes