41 research outputs found
Molecular confirmation of ovine herpesvirus 2-induced malignant catarrhal fever lesions in cattle from Rio Grande do Norte, Brazil
Mapeamento de alunos surdos matriculados na rede de ensino pública de um município de médio porte do Estado de São Paulo: dissonâncias
Identificação botânica e química de espécies vegetais de uso popular no Rio Grande do Norte, Brasil
Psychology and aggression
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68264/2/10.1177_002200275900300301.pd
Otimização de protocolo para micropropagação da figueira "Roxo de Valinhos" Protocol otimization for micropropagation of 'Roxo de Valinhos' fig tree
Foram avaliados os efeitos de alterações na concentração de sacarose, do meio WPM - Wood Plant Medium - e da variação no número de gemas por segmento com diferentes doses de cinetina. Os experimentos foram conduzidos no Laboratório de Cultura de Tecidos da Universidade Federal de Lavras, utilizando plântulas previamente estabelecidas in vitro, seguindo o delineamento experimental inteiramente casualizado, em esquema fatorial 5 x 4. Os resultados mostraram que, na multiplicação de brotações de figueira "Roxo de Valinhos", podem-se usar 100% do meio WPM com adição de 10gL-1 de sacarose. Foram obtidos brotos alongados quando foi usado o meio WPM sem adição de cinetina e segmentos com uma ou duas gemas. A adição de cinetina a 0,5mgL-1 e a utilização de segmentos com três gemas promoveu maior número de brotações.<br>Effects of different concentration of sucrose on Wood Plant Medium -WPM and variations on number of bud/plantlets with different doses of kinetin were evaluated on this research. The experiments were carried out in the Tissue Culture Laboratory at Federal University of Lavras, using plantlets which was already established in vitro. The experimental design adopted was the complete randomized in factorial scheme 5 x 4. The results revealed that better sprouts multiplication of ‘Roxo de Valinhos' fig plants occurred in WPM medium, 100% salts, added with 10gL-1 of sucrose and the sprout elongation in WPM medium without kinetin taking explants within one or two buds. Adding 0.5mgL-1 of kinetin and taking explants with tree buds promoted larger sprouts number
Expression of J chain mRNA in duodenal IgA plasma cells in IgA nephropathy
Expression of J chain mRNA in duodenal IgA plasma cells in IgA nephropathy. Glomerular IgA in IgA nephropathy (IgAN) is at least in part polymeric, and is thought to derive from the mucosal IgA system in view of the association between mucosal infection and haematuria in this condition. To investigate this hypothesis, an in situ hybridization (ISH) technique was developed for the detection of J chain mRNA, the expression of which has been correlated with the secretion of high level polymeric immunoglobulin (pIg). Endoscopic duodenal biopsies from ten patients and matched controls were examined by: (i) two color immunofluorescence (IF); (ii) ISH; and (iii) combined ISH and IF, to permit simultaneous identification of plasma cell type. IF revealed a reduction in the percentage of IgA plasma cells (P < 0.02) and increased absolute numbers of IgG cells (P < 0.02) in patient biopsies. ISH demonstrated fewer J chain mRNA expressing plasma cells (P < 0.005) with lower signal intensity (P < 0.002) in patients' biopsies compared with controls. Combined ISH and IF confirmed a reduction in J chain mRNA-positive IgA plasma cells in the patient biopsies (P < 0.02). The reduction in J chain mRNA expression in duodenal IgA plasma cells in IgAN argues against the gastrointestinal lamina propria as the source of glomerular pIgA