Detection of mutant p53 in hepatocellular cancer from turkey and its correlation with clinicopathologic parameters

WOS: 000181980500005PubMed ID: 12772781The samples of hepatocellular carcinoma from Turkey, a country with a high prevalence of hepatitis B virus and hepatitis C virus, but low dietary exposure to aflatoxin B1, were examined in order to detect the frequency of mutant p53 and its association with clinical and pathological data. Fifty-two samples of hepatocellular cancer from the patients who were diagnosed in our clinic were included in this study. The mutant p53 protein was searched for by specific enzyme-linked immunosorbent assay. Of 52 patients with hepatocellular carcinoma, 26 (50%) had the mutant p53. The incidence of p53 mutation in hepatocellular cancer patients with chronic liver disease due to hepatitis B virus infection was significantly higher than in those with chronic liver disease due to alcohol, indicating that not alcohol but hepatitis B virus, in fact induces the mutations in p53 gene. In addition, it has been shown that the p53 mutation was significantly associated with the diameter of tumor nodule and the degree of cellular differentiation in hepatocellular cancer. The p53 mutation rate found in our study is concordant for a geography where hepatitis B virus and hepatitis C virus are common. Hepatitis B virus and possibly hepatitis C virus, but not alcohol, should be responsible, to a degree, for the mutational change in p53 protein in hepatocellular cancer patients with chronic liver disease. The p53 mutation is a late event in hepatocarcinogenesis because it is related with cellular differentiation and tumor diameter. The specific ELISA can be a useful screening test in future studies to select the patients for gene therapy using wild-type p53

Novel hybridization indicator methylene blue for the electrochemical detection of short DNA sequences related to the hepatitis B virus

WOS: 000089671500003A novel assay for the voltammetric detection of DNA sequences related to the hepatitis B virus (HBV), using MB as the hybridization indicator was performed. The voltammetric signals of MB have been investigated at bare CPE, double stranded DNA (dsDNA)-modified CPE and single stranded DNA (ssDNA)-modified CPE by means of differential pulse voltammetry and cyclic voltammetry and the increased peak currents were observed, in respect to the order of electrodes. The extent of hybridization between the 21-mer oligonucleotides of complementary sequences was determined by the enhancement of the voltammetric signal of MB. The response of the hybridization of the probe with the single-base mismatch oligonucleotide at CPE was detected by MB. In this case, the unbound guanine bases increased the voltammetric signal obtained with the hybrid-modified CPE. Control experiments with the noncomplementary oligonucleotides were performed to assess whether the DNA. biosensor responds selectively, via hybridization, to the target. Numerous factors, affecting the probe immobilization, target hybridization and indicator binding reactions are optimized to maximize the sensitivity and reduce the assay time. The new indicator MB holds great promise for rapid screening of HBV infection. (C) 2000 Elsevier Science B.V. All rights reserved

DNA electrochemical biosensor for the detection of short DNA sequences related to the hepatitis B virus

WOS: 000081368800011Nucleic acid hybridization forms the basis for the diagnosis of genetic and infectious diseases. Electrochemical biosensors, coupling the inherent specificity of DNA recognition reactions with the high sensitivity of physical transducers, thus hold great promise for sequence-specific detection. An electrochemical biosensor for the voltammetric detection of DNA sequences related to the hepatitis B virus (HBV) is described. Synthetic single-stranded oligonucleotides ("probe") have been immobilized onto carbon paste electrodes with the adsorption at a controlled potential. The probes were hybridized with different concentrations of complementary ('target') sequences. The formed hybrids on the electrode surface were evaluated by differential pulse voltammetry using cobalt phenanthroline, [Co(phen)(3)(3+)] as the indicator of hybridization reaction

Possible role of glutathione in prevention of acetaminophen-induced hepatotoxicity enhanced by fish oil in male Wistar rats

WOS: 000072469800004PubMed ID: 9482353It has been reported that fish oil protects the rat liver against acetaminophen (APAP) induced toxicity; however, this finding is controversial. The present study was undertaken to investigate the effects of fish oil-enriched diet on APAP-induced liver injury in Wistar rats. Rats were fed a diet supplemented with either 8% fish oil or 8% corn oil, or standard rat feed for 6 wk. After an overnight fast, rats in each group were given either 2 g/kg APAP or saline orally. Our findings showed that APAP increased serum alanine aminotransferase (ALT) and that this rise was potentiated in the presence of dietary fat. Further fish oil ingestion increased the glutathione (GSH) content in rat liver; however, this was not effective in protecting liver from APAP-induced toxicity. Data suggest that GSH may be necessary to detoxify APAP metabolites, which are known to induce hepatotoxicity but are increased by dietary fat

Treatment of non-alcoholic steatohepatitis with N-acetyl cystein.

WOS: 00008678410162