Karakterisasi Bakteri Potensial Pendegradasi Oli Bekas Pada Tanah Bengkel Di Kota Padang

The research objective is to locate and determine the morphological and biochemical characteristics of the bacteria that could potentially degrade the used lubricant oil in the workshops in Padang. The research was conducted March to October 2016. The bacteria obtained from the workshop is cultured on selective media MSM and transferred to LB medium to obtain pure isolates. Morphological and biochemical characterization indicate three types of bacteria that live in workshop's soil contaminated used lubricant oil, namely Bacillus sp1, sp2 and Alcaligenes Bacillus s

Pengaruh Volume Cairan Rumen Sapi Terhadap Bermacam Feses Dalam Menghasilkan Biogas

This research is aimed at investigating the influence of cow's rumen fluid volume toward varied feces in producing biogas. This research has been done since October to December 2013 at Biotechnology laboratory, Faculty and Mathematic Science. The method of this research was completely randomized design (CRD) with factorial in three times. The first factor as the treatment was the type of feces, such as cow, horse, goat, and buffalo's feces. The second factor was the treatment by adding the volume of cow's rumen liquid as many as 160 and 320 ml. The control was the treatment without adding the volume of cow's rumen liquid. The variables that were measured in this research were the volume of biogas and the duration of biogas flame that could be produced. The result of this research shows that the type of feces affected the biogas production. In these treatments, the highest volume of biogas that was produced to the lowest were horse's feces yield of 226.56 cm3, cow's feces yield of 71.40 cm3, buffalo's feces yield of 28.28 cm3, and goat's feces yield of 2.13 cm3. The addition of rumen liquid can increase the biogas production on cow, goat, and buffalo's feces, but it did not significantly give the effect on horse's feces. The positive interaction between addition of rumen liquid and feces shows on cow, goat, and buffalo's feces. The rumen liquid, however, was not interacted with horse's feces for producing biogas

Optimation of Ethanol Extract of Centella Asiatica and Cresintia Cujete Composition as Natural Antioxidant Source

Pengembangan antioksidan alami dalam pemanfaatan pegagan (Centella asiatica) dan majapahit (Cresintia cujete) merupakan potensi yang besar. Penelitian ini telah melakukan optimasi komposisi ekstrak etanol pegagan (Centella asiatica) dan majapahit (Cresintia cujete) sebagai sumber antioksidan dengan dilakukan uji aktifitas penangkapan radikal bebas terhadap DPPH. Hasil penelitian menunjukkan bahwa baik ekstrak etanol pegagan (Centella asiatica) dan majapahit (Cresintia cujete) memiliki kandungan total fenol yang ditunjukkan dengan adanya kemampuan mereduksi reagen Folin Ciocalteu menjadi berwarna biru. Hasil optimasi komposisi ekstrak pegagan (Centella asiatica): majapahit (Cresintia cujete) = 30:70 memiliki aktifitas penangkapan radikal bebas terhadap DPPH yang paling baik dibandingkan ekstraks etanol pegagan (Centella asiatica) dan majapahit (Cresintia cujete). Nilai IC50 untuk komposisi ekstrak etanol pegagan (Centella asiatica): majapahit (Cresintia cujete) = 30:70 sebesar 0,103 mg/mL

Pengembangan Media Pembelajaran Berbasis Android pada Materi Transkripsi dan Translasi untuk Mata Kuliah Genetika

Materi biologi banyak menjelaskan konsep abstrak seperti proses fisiologis hingga molekuler membuat materi biologi dianggap sulit oleh mahasiswa. Berdasarkan hasil observasi dan wawancara yang telah dilakukan, masih banyak mahasiswa menganggap materi pada matakuliah genetika sulit terutama pada materi transkripsi dan translasi. Mahasiswa membutuhkan media vang praktis untuk digunakan dimanapun dan kapanpun serta mampu memvisualisasikan materi tersebut. Media pembelajaran berbasis Android diharapkan mampu meningkatkan pemahaman mahasiswa terhadap materi transkripsi dan translasi. Penelitian ini merupakan Research and Development menggunakan model 4D, namun pada penelitian ini dibatasi hanya tiga tahapan yaitu Define, Design dan Develop, khususnya sampai validasi produk. Produk media yang dikembangkan berupa media pembelajaran dengan format aplikasi .apk yang bisa dioperasikan pada perangkat smartphone Android dan pada perangkat laptop/PC menggunakan software emulator seperti NOX dan lainnya. Media pembelajaran ini memuat materi transkripsi dan translasi yang terdiri dari menu-menu yaitu: Learning Outcomes (LO), materi (memuat penjelasan serta proses transkripsi dan translasi), latihan (berupa soal-soal dalam bentuk multiple choises), dan tombol keluar. Berdasarkan uji validitas didapatkan hasil validiasi dari ahli materi 97,5% dengan kategori sangat valid dan ahli media juga dapat dikategorikan sangat valid sebesar 83.8%. Berdasarkan hasil tersebut didapatkan angka validitas sebesar 91,2% dengan kategori sangat valid

Analisis Filogenetik Ikan Tuna (Thunnus Spp) Di Perairan Maluku Utara Menggunakan Coi (Cytocrome Oxydase I)

Artikel ini bertujuan untuk menjelaskan kekerabatan atau filogeni ikan tuna(Thunnus spp) menggunakan COI (Chytocrome Oxydase I). Metode dalam penelitian ini menggunakan data sekunder dari situs web NCBI ((Pusat Informasi Bioteknologi Nasional). Data yang diambil yaitu urutan nukleotida dari Sitokrom Oksidasegen I (COI) dalam DNA mitokondria. Spesies thunnus yang dianalisis adalah thunuss dari hasil identifikasi di Perairan Maluku Utara, terdiri dari Thunnus obesus, Thunnus albacar, Thunnus alalunga,dan Katsuwonus pelamis. Dari hasil analisis dengan menggunakan software MEGA X didapatkan tingkat kekerabatan antar spesies yang diuji sangat dekat, antara lain spesiesthunnus obesus, T. albacar, T. alalunga dan Katsuwons pelamis. Jarak rata-rata genetik dari semua spesies adalah 0,010. Secara umum keterkaitan spesies yang ditemukan adalah beberapa spesies yang ditemukan di lokasi yang sama dengan morfologi dan makanan yang hampir sama. Untuk peneliti selanjutnya, diharapkan ada tambahan famili dari spesies ikan akan dianalisis untuk analisis filogenetik di Perairan Maluku Utara, sehingga mereka dapat mengetahui hubungan beberapa jenis lainnya. Kata kunci : Analisis filogenetik, Thunuss, Ikan tuna, Penanda CO

FSH receptor gene haplotype distribution in normozoospermic and azoospermic men.

The human follicle-stimulating hormone (FSH) receptor (FSHR) gene possesses single nucleotide polymorphisms (SNP) in exon 10, which influence serum FSH levels in women, but not in men. In the present study we extend our previous investigation and for the first time analyze a novel, common SNP at position -29 of the FSHR core promoter in men. The SNP in codon 680 was analyzed in 438 men with nonobstructive azoospermia and in 304 controls. The SNP in codon 307 and at position -29 was analyzed in 345 men with nonobstructive azoospermia and 186 controls. SNPs were determined by allelic discrimination. No significant difference in the frequency of the polymorphism at position 680 and serum FSH levels was found. At position -29 (A/G) the A-29 allele was less frequent than the G-29 allele both in controls (25% vs 75%) and in patients (30% vs 70%) (P not signficant). Together the three SNPs form four discrete haplotypes (A-Thr-Asn, G-Thr-Asn, A-Ala-Ser, and G-Ala-Ser) occurring in 10 combinations. A statistically significant difference in the allelic distribution between controls and azoospermic men was found (P < .05 by {chi}2 test). The A-Ala-Ser allele was more frequent in patients (9.1%) than in controls (5.4%), whereas the G-Thr-Asn allele was less frequent in patients (33.1%) than in controls (40.6%) (P < .01 by Fisher's exact test). No significant correlation between serum FSH levels and FSHR allele was found. We conclude that the FSHR haplotype does not associate with different serum FSH levels but it is differently distributed in normal and azoospermic men. The A-Ala-Ser and the G-Thr-Asn allele might represent genetic factors contributing to phenotypic expression of severe spermatogenetic impairment

Single nucleotide polymorphisms in the promoter region influence the expression of the follicle-stimulating hormone receptor.

OBJECTIVE: To characterize novel single-nucleotide polymorphisms (SNPs) in the human FSH receptor (FSHR) promoter region. DESIGN: Retrospective and basic research study. SETTING: University hospital. PATIENTS: Women (202 from Germany and 55 from Indonesia) with male or tubal factor infertility undergoing controlled ovarian stimulation for IVF treatment. INTERVENTIONS: None. MAIN OUTCOME MEASURE(S): Frequency, distribution, and correlation with clinical data of the SNPs. Dual luciferase assays and electrophoretic mobility shift assays (EMSA). RESULT(S): We identified two SNPs and three mutations in the promoter region of the human FSHR which could be allocated to positions -29, -37, -114, -123, and -138 upstream of the translational initiation codon. One SNP showed a high incidence (-29: 44%, n = 202), but no correlation with basal FSH serum levels or ovarian response with the SNP at position -29 was found. Luciferase reporter assays, using pGL3 vector constructs, showed that mutations at positions -37 and -138 lead to significantly higher promoter activity. EMSA indicate that putative binding sites for transcription factors are affected by the SNPs. CONCLUSIONS: The newly identified SNPs do not seem to influence clinical parameters substantially, but modulate expression of the FSHR via changes in transcription factor binding sites