Analysis of Procyanidins in Chocolate by Reversed-Phase High-Performance Liquid Chromatography with Electrospray Ionisation Mass Spectrometric and Tandem Mass Spectrometric Detection.

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Quantitative Analysis of Tissues of the Central Nervous System in Food Products by GFAP-ELISA Test Kit. Results of an Interlaboratory Study.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

In-house Validation of an Improved Sample Extraction and Clean-up for GC Determination of Isomers of Nervonic Acid in Meat Products.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

Results of an Inter-laboratory Validation Study of Five Commercial ELISA Test Kits for the Determination of Peanut Proteins in Biscuits and Dark Chocolate

The results of an inter-laboratory study with five commercially available peanu ELISA test kits to detects and quantify peanut residues in two food matrices (biscuit and dark chocolate) at four different concentrations( O-10 mg peanut kg-1 matrix corresponding to about 0-2.5 mg peanut protein kg-1 matrix) are reported. In general the fice ELISA test kits evaluated could detect peanut protein in the two food matrices. In three cases, the study challenged the test kits beyond their intended use for quantification below the manufacturers' defined cut-off limits. Generally, all five ELISA test kits performed well in the concentration range 5-10 mg kg-1 rather than in the low concentrations range (2.0 to 2.5 mg kg-1). The variation in the found recoveries of peanut between the different test kits had a spread of 44-191% across all concentrations. The quantification characteristics between test kits differed significantly at the very low mg kg-1 level. Two test kits performed well even at concentrations below 5 mg kg-1 with reproducibilities ot 27-36% for biscuits and 45-57% for chocolate.JRC.D.8-Food safety and qualit

Oxygen targets and 6-month outcome after out of hospital cardiac arrest: a pre-planned sub-analysis of the targeted hypothermia versus targeted normothermia after Out-of-Hospital Cardiac Arrest (TTM2) trial

International audienceAbstract Background Optimal oxygen targets in patients resuscitated after cardiac arrest are uncertain. The primary aim of this study was to describe the values of partial pressure of oxygen values (PaO 2 ) and the episodes of hypoxemia and hyperoxemia occurring within the first 72 h of mechanical ventilation in out of hospital cardiac arrest (OHCA) patients. The secondary aim was to evaluate the association of PaO 2 with patients’ outcome. Methods Preplanned secondary analysis of the targeted hypothermia versus targeted normothermia after OHCA (TTM2) trial. Arterial blood gases values were collected from randomization every 4 h for the first 32 h, and then, every 8 h until day 3. Hypoxemia was defined as PaO 2  300 mmHg. Mortality and poor neurological outcome (defined according to modified Rankin scale) were collected at 6 months. Results 1418 patients were included in the analysis. The mean age was 64 ± 14 years, and 292 patients (20.6%) were female. 24.9% of patients had at least one episode of hypoxemia, and 7.6% of patients had at least one episode of severe hyperoxemia. Both hypoxemia and hyperoxemia were independently associated with 6-month mortality, but not with poor neurological outcome. The best cutoff point associated with 6-month mortality for hypoxemia was 69 mmHg (Risk Ratio, RR = 1.009, 95% CI 0.93–1.09), and for hyperoxemia was 195 mmHg (RR = 1.006, 95% CI 0.95–1.06). The time exposure, i.e., the area under the curve (PaO 2 -AUC), for hyperoxemia was significantly associated with mortality ( p = 0.003). Conclusions In OHCA patients, both hypoxemia and hyperoxemia are associated with 6-months mortality, with an effect mediated by the timing exposure to high values of oxygen. Precise titration of oxygen levels should be considered in this group of patients. Trial registration : clinicaltrials.gov NCT02908308 , Registered September 20, 2016

Ventilatory settings in the initial 72 h and their association with outcome in out-of-hospital cardiac arrest patients: a preplanned secondary analysis of the targeted hypothermia versus targeted normothermia after out-of-hospital cardiac arrest (TTM2) trial

International audienc