ANNONA MURICATA LINN. ETHANOLIC LEAF EXTRACT AMELIORATES REPRODUCTIVE COMPLICATIONS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS

Diabetes mellitus is an endocrine and metabolic disorder of humans and animals characterized by hyperglycemia and low blood insulin levels or insensitivity of target organs to insulin and it’s a major health problem affecting patient’s quality of life due to its many complications. Infertility is one of the major secondary complications in diabetes. Although numerous drugs have been used for intervention studies on diabetes-induced infertility worldwide, there are currently no treatments for diabetes associated infertility in humans. This study was performed to investigate the effects of Annona muricata ethanolic leaf extract (AMELE) on fertility of male diabetic rats and levels of blood glucose. Twenty male Wistar rats (150-200g) were randomly distributed into 4 groups (n=5) treated thus: CTRL (control), DNT, DT1 and DT2 (diabetic, single intraperitoneal injection, streptozotocin, 60 mg/kg). Group DT1 and DT2 received AMELE orally at 100 mg/kg and 200 mg/kg respectively daily for fourteen days. Data were analysed using ANOVA at α0.05. The animals were sacrificed after 2 weeks via thiopental injection and testicular weights were recorded. Fasting blood glucose was determined using a digital glucometer. Sperm count, motility, viability and morphology were assessed microscopically. Testes were histologically evaluated. The results showed that oral administration of AMELE at 100 mg/kg and 200 mg/kg to diabetic male rats for fourteen days significantly decreased blood glucose level and also ameliorated diabetes-induced decreases in sperm functions in streptozotocin-induced diabetic male rats.   &nbsp

Genomic exploration of Bacillus thuringiensis MORWBS1.1 - candidate biocontrol agent,predicts genes for biosynthesis of zwittermicin, 4,5-DOPA dioxygenase extradiol, and quercetin 2,3-dioxygenase

Many strains fromBacillus thuringiensisare known for theirgenomic robustness and antimicrobial potentials. As a result, thequest for their biotechnological applications, especially in theagroindustry (e.g., as biopesticides), has increased over the years.This study documents the genome sequencing and probing of aFusariumantagonist (B.thuringiensisstrain MORWBS1.1) withpossible biopesticidal metabolite producing capacity from SouthAfrica. Based on in vitro evaluation and in silico antiSMASHinvestigation,B.thuringiensisstrain MORWBS1.1 exhibited dis-tinctive genomic properties that could be further exploited for inplanta and food additive production purposes