The Familial State: Elite Family Practices and State-Making in the Early Modern Netherlands

http://deepblue.lib.umich.edu/bitstream/2027.42/51233/1/467.pd

Trading States, Trading Places: The Role of Patrimonialism in Early Modern Dutch Development

http://deepblue.lib.umich.edu/bitstream/2027.42/51281/1/517.pd

How Politics Can Shape Sports and the Athletes Who Perform: A Case Study of Hungarian Gymnastics During the Cold War

The purpose of the project is to juxtapose how politics and government structure shape the world of sports, as well as the progress of individual sports, with the isolation of athletes from the political realities of their respective nations. We assert that contemporary politics is directly reflected in the sports community through the way athletes interact and the ways other nations perceive athletes’ treatment. We argue that around the Hungarian Revolution, Eastern countries like Hungary attempted to perform their strength by giving privileges that allowed athletes to excel while western nations like the United States focused on fostering their savior complexes by saving and subsequently parading around eastern athletes who were convinced to defect. This narrative is complicated by the disparity between the sports community acknowledging how domestic and international politics influences each sport and the utopia view that governments were able to craft among their athlete populations about the government\u27s actions.https://digitalcommons.ursinus.edu/cold_war_lives/1002/thumbnail.jp

Marginality in Inquiry-Based Science Learning Contexts: The Role of Exclusion Cascades

Vygotskyan-inspired theories of learning have been applied in science education research, yet to more explicit attention to links between local social interactions and cultural-historical processes is needed advance critical theories of science learning. This microgenetic case study examined identity and motivation processes in a 7th grade inquiry science context with the goal of better historicizing these processes by describing the phenomenon of exclusion cascades in relation to two backgrounded cultural-historical processes, alienation and the social division of labor. Exclusion cascades highlighted the mutual constitution of competence and belonging. Implications are discussed with respect to challenging adaptationist ethos in science education

Self-Assessment and Planned Change of Placement and Career Services Center

In the 1990s, college and university career services and placement units face many challenges which may influence their success including (a) increased budgetary constraints, (b) changing student demographics, (c) increased availability of computer technologies, (d) new service delivery models, and (e) changing employer recruitment practices. In an effort to address these issues and examine its role within the university (Roth, 1994), the Placement and Career Information Center (PCIC) at Central Michigan University undertook a program of applied research, self-assessment and planned change. The purpose of this article is to briefly report our experiences in conducting this program of applied research. The assessment strategy is presented in the first part of this report. Next, the methods used to collect data and assessment, sampling procedures and response rates are described. Following this, highlights of the assessment results are presented including a summary of some changes already made and those planned for the future. The results of this study are presented in detail in Adams, et at. (1994)

UAlbany Faculty OER Showcase, Catherine Dwyer Director of Scholarly Communications

Julia Hastings presentation: 0:00-16:05 Elise Gervais presentation: 16:05-26:06 Marcus Adams presentation: 26:06-41:45 The slides that Marcus Adams used for his presentation are available for download

The familial state: Elite family practices and state-making in the early modern Netherlands

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43648/1/11186_2004_Article_BF00992826.pd

Etablierung eines GMP-konformen Zellkultur- und Expansionsprotokolls zur Vorbereitung einer klinischen Phase-1-Studie einer adoptiven Immuntherapie maligner Erkrankungen mit der natürlichen Killerzelllinie (NK-92)

Die natürliche Killerzelllinie NK-92 zeichnet sich durch eine breit gefächerte Aktivität gegen verschiedenste Tumore und Leukämien aus und würde sich daher prinzipiell für eine Verwendung als adoptives Zelltherapeutikum eignen. NK-92-Zellen sind eine von nur 5 etablierten NK-Zelllinien weltweit. Ihr Wachstum in der Zellkultur war bisher von Bedingungen abhängig, die mit einer klinischen Anwendung der Zellen nicht zu vereinbaren sind. Ziel der vorliegenden Doktorarbeit war es daher, ein Kulturverfahren zu etablieren, mit dem sich NK-92-Zellen unter Bedingungen einer „Guten Herstellungs Praxis“ kultivieren und expandieren lassen. In dieser Arbeit wurde daher die Adaption der NK-92-Zellen an ein in der Klinik einsetzbares Zellkulturmedium vorgenommen und ein Batch-Kulturverfahren entwickelt, mit dem sich die NK-92-Zellen innerhalb von 10-14 Tagen auf bis zu 1010 Zellen in 10L Kulturvolumen expandieren lassen. Die Funktionsprüfung der NK-92-Zellen, anhand der Expression von immunologisch relevanten Oberflächenrezeptoren (CD11a, CD25, CD28, CD54, CD56, CD122, FAS-L), ergab keine Veränderung des Phänotyps der expandierten Zellen. Darüber hinaus wiesen die Zellen eine Viabilität von >95,3% +/- 0,46% auf, und ihre zytotoxische Aktivität gegen die NK-sensitive Leukämiezelllinie K562 war nicht eingeschränkt. Da NK-92-Zellen in der Erkennung virusinfizierter und maligner Zellen nicht MHCrestringiert sind, eignen sie sich auch für den ungerichteten Einsatz. Hierzu wäre eine Expansion der Zellen im großen Massstab mit anschliessender Kryokonservierung von Vorteil, da die Zellen dann im Voraus hergestellt und geprüft werden könnten. Die Prüfung des Einflusses unterschiedlicher Konzentrationen (0, 0,5, 1, 2, 3, 5, 8 10 %) der Einfrierschutzlösung Dimethylhylsulfoxid (DMSO) auf die zytotoxische Aktivität der NK-92-Zellen ergab keine Einschränkung der NKZellfunktion bei Konzentrationen 95,3% +/- 0,46%, and no impairement with regard to their cytotoxic activity against the NK-sensitive leukemia cell line K562. Since the immune response of NK cells against virus infected or malignant cells is not MHC-restricted, they thus hold the option for a readily available “off the shelf” anti-cancer cell therapy. For this, an expansion of the cells on a large scale basis with concomitant cryoconservation would be of advantage and allow the in advance production of NK-92 batches with defined quality. The examination of the influence of different concentrations (0, 0,5, 1, 2, 3, 5, 8 10 %) of the freezing solution Dimethylhylsulfoxid (DMSO) on the cytotoxic activity of the NK-92 cells, revealed that concentrations of <5% had no impact on the NK cell function. As a result, different freezing protocols were examined with regard to their influence on the viability of NK-92 cells. The NK-92 cells were cryopreserved in cryovials with 2, 3, 5, 8 and 10% DMSO in human serum albumin (HSA), respectively. Clinical scale doses of NK-92 (5x108 cells/20ml HSA) were cryopreserved in freezing bags with 3, 5 and 10% DMSO, respectively. After thawing NK-92 cells were tested for their viability. Overall, the cryopreservation and thawing of NK-92 cells resulted in considerably low viabilities irrespective of freezing protocol and/or DMSO concentration used, with mean viabilities of < 50% +/- 9,77. For a clinical application, NK-92 cells have to be irradiated with 10 Gy. It was of no significance for the viability of cryopreserved NK-92 cells, whether the irradiation was performed before freezing or after thawing (Viability 48,8% vs. 44%). Based on this thesis, a concept was developed that allows the expansion of NK-92 cells to clinical scale, resulting in functional highly active NK-92, while providing outmost safety standards for the patients. Starting from a master cell bank, this concept assumes the expansion of NK-92 cells in 2L Nunc-CellfactoryÒ culture flasks under batch culture conditions. The cultures are initiated with 2x104 NK-92 cells/ml in X-Vivo10 Medium, supplemeted with 5% fresh frozen plasma (FFP) and 100 IE IL-2. On day 10 the batch cultures have reached their maximum density (6,4 x105/ml )

Fermi surface, possible unconventional fermions, and unusually robust resistive critical fields in the chiral-structured superconductor AuBe

The noncentrosymmetric superconductor (NCS) AuBe is investigated using a variety of thermodynamic and resistive probes in magnetic fields of up to 65~T and temperatures down to 0.3~K. Despite the polycrystalline nature of the samples, the observation of a complex series of de Haas-van Alphen (dHvA) oscillations has allowed the calculated bandstructure for AuBe to be validated. This permits a variety of BCS parameters describing the superconductivity to be estimated, despite the complexity of the measured Fermi surface. In addition, AuBe displays a nonstandard field dependence of the phase of dHvA oscillations associated with a band thought to host unconventional fermions in this chiral lattice. This result demonstrates the power of the dHvA effect to establish the properties of a single band despite the presence of other electronic bands with a larger density of states, even in polycrystalline samples. In common with several other NCSs, we find that the resistive upper critical field exceeds that measured by heat capacity and magnetization by a considerable factor. We suggest that our data exclude mechanisms for such an effect associated with disorder, implying that topologically protected superconducting surface states may be involved