176 research outputs found
Difference in trafficking of brain-derived neurotrophic factor between axons and dendrites of cortical neurons, revealed by live-cell imaging
BACKGROUND: Brain-derived neurotrophic factor (BDNF), which is sorted into a regulated secretory pathway of neurons, is supposed to act retrogradely through dendrites on presynaptic neurons or anterogradely through axons on postsynaptic neurons. Depending on which is the case, the pattern and direction of trafficking of BDNF in dendrites and axons are expected to be different. To address this issue, we analyzed movements of green fluorescent protein (GFP)-tagged BDNF in axons and dendrites of living cortical neurons by time-lapse imaging. In part of the experiments, the expression of BDNF tagged with cyan fluorescent protein (CFP) was compared with that of nerve growth factor (NGF) tagged with yellow fluorescent protein (YFP), to see whether fluorescent protein-tagged BDNF is expressed in a manner specific to this neurotrophin. RESULTS: We found that BDNF tagged with GFP or CFP was expressed in a punctated manner in dendrites and axons in about two-thirds of neurons into which plasmid cDNAs had been injected, while NGF tagged with GFP or YFP was diffusely expressed even in dendrites in about 70% of the plasmid-injected neurons. In neurons in which BDNF-GFP was expressed as vesicular puncta in axons, 59 and 23% of the puncta were moving rapidly in the anterograde and retrograde directions, respectively. On the other hand, 64% of BDNF-GFP puncta in dendrites did not move at all or fluttered back and forth within a short distance. The rest of the puncta in dendrites were moving relatively smoothly in either direction, but their mean velocity of transport, 0.47 ± 0.23 (SD) μm/s, was slower than that of the moving puncta in axons (0.73 ± 0.26 μm/s). CONCLUSION: The present results show that the pattern and velocity of the trafficking of fluorescence protein-tagged BDNF are different between axons and dendrites, and suggest that the anterograde transport in axons may be the dominant stream of BDNF to release sites
A Numerical and experimental study on the crash behavior of the extruded aluminum crash box with elastic support
Non-collinear interaction of guided elastic waves in an isotropic plate
The nonlinear wave propagation in a homogeneous and isotropic elastic plate is analyzed theoretically to investigate the non-collinear interaction of plate wave modes. In the presence of two primary plate waves (Rayleigh-Lamb or shear horizontal modes) propagating in arbitrary directions, an explicit expression for the modal amplitude of nonlinearly generated wave fields with the sum or difference frequency of the primary modes is derived by using the perturbation analysis. The modal amplitude is shown to grow in proportion with the propagation distance when the resonance condition is satisfied, i.e., when the wavevector of secondary wave coincides with the sum or difference of those of primary modes. Furthermore, the non-collinear interaction of two symmetric or two antisymmetric modes is shown to produce the secondary wave fields consisting only of the symmetric modes, while a pair of symmetric and antisymmetric primary modes is shown to produce only the antisymmetric modes. The influence of the intersection angle, the primary frequencies, and the mode combinations on the modal amplitude of secondary wave is examined for a low-frequency range where the lowest-order symmetric and antisymmetric Rayleigh-Lamb waves and the lowest-order symmetric shear horizontal wave are the only propagating modes
Second-harmonic generation in a multilayered structure with nonlinear spring-type interfaces embedded between two semi-infinite media
The acoustic second-harmonic generation behavior in a multilayered structure with nonlinear spring-type interlayer interfaces is analyzed theoretically to investigate the frequency dependence of second-harmonic amplitudes in the reflected and transmitted fields when the structure is subjected to the normal incidence of a monochromatic longitudinal wave. The multilayered structure consists of identical linear elastic layers and is embedded between two identical linear elastic semi-infinite media. The layers are bonded to each other by spring-type interfaces possessing identical linear stiffness but different quadratic nonlinear parameters. By combining a perturbation analysis with the transfer-matrix method, analytical expressions are derived for the second-harmonic amplitudes of the reflected and transmitted waves. The second-harmonic amplitudes due to a single nonlinear interface are shown to vary remarkably with the fundamental frequency, reflecting the pass and stop band characteristics of the Bloch wave in the corresponding infinitely extended layered structure. By calculating the spatial distribution of second-harmonic amplitude inside the multilayered structure, the influence of the position of the nonlinear interface as well as the number of layers on the frequency dependence of second-harmonic amplitudes of the reflected and transmitted waves is elucidated. When all interlayer interfaces possess the identical nonlinearity, the second-harmonic amplitudes on both sides of the structure are shown to increase monotonically with the number of layers in the frequency ranges where both fundamental and double frequencies are within the pass bands of Bloch wave. The influence of two non-dimensional parameters, i.e., the relative linear compliance of the interlayer interfaces and the acoustic impedance ratio between the layer and the surrounding semi-infinite medium, on the second-harmonic amplitudes is elucidated
Application of an Alternative Measuring System to Determine the Mechanical Properties of Composites under Impact
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