Humoral markers of severity and prognosis of critical illness

Despite the considerable advances made in understanding the pathophysiology of systemic inflammation during critical illness, clinical progress has been elusive as it remains a very deadly condition. Cortisol and thyroid hormone levels can be as predictive of outcome as the commonly used severity parameters (i.e. APACHE). Indeed, levels of endocrine humoral substances such as arachidonic acids, nitric oxide, endothelin, calcitonin, precursors, leptin and adenosine correlate with the severity and outcome of critical illness. Furthermore, calcitonin precursors represent a potentially new hormokine paradigm, being transcriptionally activated in all cells in response to infection. The cytokines are immune markers that often correlate with severity and outcome, but their release is transient. In contrast, the so-called acute phase proteins, such as C-reactive protein and serum amyloid A, are highly sensitive to inflammatory activity and can be important markers of severity and outcome. Leukocyte esterase, adhesion molecules, platelet activating factor and activated protein C are additional humoral immune markers; the replacement of the latter has been shown to be a promising therapeutic option. Natriuretic peptides are neurocrine humoral markers that have important cardiovascular implications. The level of macrophage migrating inhibitory factor, released by the pituitary, is elevated in sepsis and counteracts glucocorticoid action. Cellular markers to severe stress include the enhanced expression of protective substances in the form of heat shock proteins. High mobility group-I is a DNA-binding protein and a late mediator of the inflammatory response. Apoptotic markers such as the soluble fas ligand are also elevated in inflammation. In summary, during critical illness, the endocrine, immune and nervous systems elaborate a multitude of humoral markers, the roles of which merit further scrutiny in order to improve therapeutic outcome

Geophysical and Remote Sensing Assessment of Chad’s Groundwater Resources

Because of climate change and human activity, North and Central Africa are experiencing a significant water shortage. Recent advancements in earth observation technologies have made widespread groundwater monitoring possible. To examine spatial and temporal mass fluctuations caused by groundwater variations in Chad, gravity solutions from the Gravity Recovery and Climate Experiment (GRACE), climatic model outputs, and precipitation data are integrated. The results are as follows: (1) The investigated region experienced average annual precipitation (AAP) rates of 351.6, 336.22, and 377.8 mm yr−1, throughout the overall investigation period (04/2002–12/2021), Period I (04/2002–12/2011), and Period II (01/2012–12/2021), respectively. (2) Using the three gravity solutions, the average Terrestrial Water Storage Variations (ΔTWS) values are estimated to be +0.26 ± 0.04, +0.006 ± 0.10, and +0.64 ± 0.12 cm yr−1, for the overall study period, periods I, and II, respectively. (3) Throughout the full period, periods I, and II, the groundwater storage fluctuations (ΔGWS) are calculated to be +0.25 ± 0.04, +0.0001 ± 0.099, and +0.62 ± 0.12 cm yr−1, respectively after removing the soil moisture (ΔSMS) and Lake Chad water level trend values. (4) The country receives an average natural recharge rate of +0.32 ± 0.04, +0.068 ± 0.099, and +0.69 ± 0.12 cm yr−1, throughout the whole period, Periods I, and II, respectively. (5) The southern mountainous regions of Erdi, Ennedi, Tibesti, and Darfur are receiving higher rainfall rates that may recharge the northern part of Chad through the stream networks; in addition to the Lake Chad and the higher rainfall over southern Chad might help recharge the central and southern parts of the country. (6) A preferred groundwater flow path from the Kufra (Chad and Libya) to the Dakhla basin (Egypt) appears to be the Pelusium mega shear system, which trends north-east. The findings suggest that GRACE is useful for monitoring changes in groundwater storage and recharge rates across large areas. Our observation-based methodology provides a unique understanding of monthly ground-water patterns at the state level, which is essential for successful interstate resource allocation, future development, and policy initiatives, as well as having broad scientific implications for arid and semiarid countries

Geophysical and Remote Sensing Assessment of Chad’s Groundwater Resources

Because of climate change and human activity, North and Central Africa are experiencing a significant water shortage. Recent advancements in earth observation technologies have made widespread groundwater monitoring possible. To examine spatial and temporal mass fluctuations caused by groundwater variations in Chad, gravity solutions from the Gravity Recovery and Climate Experiment (GRACE), climatic model outputs, and precipitation data are integrated. The results are as follows: (1) The investigated region experienced average annual precipitation (AAP) rates of 351.6, 336.22, and 377.8 mm yr−1, throughout the overall investigation period (04/2002–12/2021), Period I (04/2002–12/2011), and Period II (01/2012–12/2021), respectively. (2) Using the three gravity solutions, the average Terrestrial Water Storage Variations (ΔTWS) values are estimated to be +0.26 ± 0.04, +0.006 ± 0.10, and +0.64 ± 0.12 cm yr−1, for the overall study period, periods I, and II, respectively. (3) Throughout the full period, periods I, and II, the groundwater storage fluctuations (ΔGWS) are calculated to be +0.25 ± 0.04, +0.0001 ± 0.099, and +0.62 ± 0.12 cm yr−1, respectively after removing the soil moisture (ΔSMS) and Lake Chad water level trend values. (4) The country receives an average natural recharge rate of +0.32 ± 0.04, +0.068 ± 0.099, and +0.69 ± 0.12 cm yr−1, throughout the whole period, Periods I, and II, respectively. (5) The southern mountainous regions of Erdi, Ennedi, Tibesti, and Darfur are receiving higher rainfall rates that may recharge the northern part of Chad through the stream networks; in addition to the Lake Chad and the higher rainfall over southern Chad might help recharge the central and southern parts of the country. (6) A preferred groundwater flow path from the Kufra (Chad and Libya) to the Dakhla basin (Egypt) appears to be the Pelusium mega shear system, which trends north-east. The findings suggest that GRACE is useful for monitoring changes in groundwater storage and recharge rates across large areas. Our observation-based methodology provides a unique understanding of monthly ground-water patterns at the state level, which is essential for successful interstate resource allocation, future development, and policy initiatives, as well as having broad scientific implications for arid and semiarid countries.Water Resource

Geophysical investigations for the identification of subsurface features influencing mineralization zones

The numerous hydrothermal alteration zones and subsurface structures affecting the mineralized deposits of the Dungash region were identified using aeromagnetic data. The Center of Exploration Targeting (CET) approach and several filters, such as reduction-to-pole, Tilt derivative, First Vertical Derivative, Horizontal gradient map, Downward continuation, analytical signal methods, regional, and residual separation, were used to analyze the aeromagnetic data. The research region is impacted by several structural trends running in the N-S, E-W, NW-SE, and NE-SW directions, and these trends are strongly related to the gold mineralization and surrounding hydrothermal alteration zones. In the NW-SE direction, four alteration zones have been identified. The research region's northern and eastern regions have shallower basement relief, with depths of only approximately 100 m, and those depths show that the area is rootless. Conversely, the basement relief and surface depths are lower in the study region's western and southern regions. The routes taken by the ascending hydrothermal fluids can be seen as aeromagnetic lineaments at the hydrothermal alteration zones. Mineralization appears to be linked to structural lineaments, as evidenced by airborne magnetic data. For gold prospecting, the aeromagnetic technique seems to be the most effective and efficient geophysical method because gold is typically found in severely deformed shear zones and faults.Water Resource

Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study

Most of the agricultural workers are potentially exposed to pesticides through different routes. Inhalation exposures may result in numerous diseases that can adversely affect an individual’s health and capacity to perform at work. The aim of this study was to determine the cytotoxic potential of cypermethrin pesticide on cultured human hepatocarcinoma (HepG2) cells. The HepG2 cells were exposed to cypermethrin (0, 5, 15, 40 ng/mL) for 24 and 48 hours. We observed that cypermethrin caused cell death of HepG2 cells using 3-(4, 5-dimethylthiozolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase tests. Furthermore, cypermethrin reduced HepG2 cells viability in a time and dose dependent basis, that was probably mediated through the induction of reactive oxygen species (ROS) and apoptosis. An increase in ROS generation with a concomitant increase in expression of the proapoptotic protein Bcl-2 and cytochrome c and decrease in the antiapoptosis protein Bax suggested that a mitochondria-mediated pathway was involved in cypermethrin-induced apoptosis. These findings provide insights into the underlying mechanisms involved in cytotoxicity of cypermethrin in HepG2 cells

Fucoidan alleviates microcystin-LR-induced hepatic, renal, and cardiac oxidative stress and inflammatory injuries in mice

International audienceFucoidans (FUCs) are sulfated polysaccharides that have a wide range of bioactivities. The current study was designed toevaluate the antioxidant potential of FUC against microcystin-LR (MC-LR)-induced toxicity. Five mice groups (n = 8) wereused. Group 1 received saline, Group 2 received oral FUC 100 mg/kg/day for 21 days, Group 3 received i.p. MC-LR 10 μg/kg/day for 14 days, Group 4 received MC-LR plus FUC 50 mg/kg/day, and Group 5 received MC-LR plus FUC 100 mg/kg/day. Thepresent study showed that MC-LR administration was associated with significant increases (p < 0.01) in serum concentrations ofhepatic (aspartate transferase, alanine transferase, and alkaline phosphatase), renal (urea and creatinine), and cardiac (creatinekinase and CK-MB) injury biomarkers, as well as serum lactate dehydrogenase, cholesterol, and pro-inflammatory cytokines(interleukins-1β and 6, and tumor necrosis factor-α), compared with the control group. Further, MC-LR-intoxicated miceexhibited significantly higher (p < 0.01) hepatic, renal, and cardiac tissue levels of malondialdehyde and nitric oxide, as wellas lower tissue levels of reduced glutathione and activities of glutathione peroxidase, superoxide dismutase, and catalase enzymesin comparison with control mice. Treatment by FUC significantly ameliorated all the above-mentioned alterations in a dosedependent manner with frequent restoration of the normal ranges in the FUC 100 mg/kg/day dose group. Moreover, treatment byFUC alone at 100 mg/kg/day was not associated with significant negative alterations in the assessed biochemical parameters,highlighting its safety at this dose. In conclusion, treatment by FUC significantly ameliorated organ injury, induced by MC-LR inmouse hepatic, renal, and cardiac tissues

The ameliorative effects of ceftriaxone and vitamin E against cisplatin-induced nephrotoxicity

International audienceNephrotoxicity is a common adverse effect of treatment with cisplatin (CDDP). This study was performed to evaluate the antioxidant and nephroprotective efficacy of ceftriaxone (CTX) and vitamin E (Vit.E), alone and in combination against CDDP-induced acute renal injury. Fifty-six male albino rats were equally divided into seven groups, receiving (I) normal saline, (II) CTX (100 mg/kg, intraperitoneal [i.p] injection), (III) Vit.E (100 mg/kg orally), (IV) CDDP (5 mg/kg i.p injection), (V) CDDP plus CTX, (VI) CDDP plus Vit.E, and (VII) CDDP plus CTX in combination with Vit.E. All treatments were administered daily for 10 days except CDDP, which was given as a single dose at the sixth day of the study. Compared to normal control rats, CDDP-injected rats showed significantly (p < 0.05) higher serum levels of renal injury biomarkers (uric acid, urea, and creatinine) and tumor necrosis factor-α (TNF-α), as well as increased renal tissue concentrations of malondialdehyde, nitric oxide, and TNF-α. Moreover, CDDP administration was associated with significantly lower (p < 0.05) renal tissue levels of reduced glutathione and activities of endogenous antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) and total antioxidant capacity. All these alterations were significantly ameliorated in CDDP-injected rats, receiving CTX and/or Vit.E, compared to rats receiving CDDP alone. Interestingly, the antioxidant and anti-inflammatory effects were more marked in the CTX-Vit.E combination group, compared to groups receivingeither drug alone. In conclusion, CTX and Vit.E (especially in combination) could counteract the nephrotoxic effect of CDDP, probably through their antioxidant activities

Association of Toll-Like Receptor 3 Single-Nucleotide Polymorphisms and Hepatitis C Virus Infection

Toll-like receptor 3 (TLR3) plays a key role in innate immunity by recognizing pathogenic, double-stranded RNAs. Thus, activation of TLR3 is a major factor in antiviral defense and tumor eradication. Although downregulation of TLR3 gene expression has been mainly reported in patients infected with hepatitis C virus (HCV), the influence of TLR3 genotype on the risk of HCV infection, HCV-related cirrhosis, and/or hepatocellular carcinoma (HCC) remains to be determined. Single-nucleotide polymorphisms (SNPs) within the TLR3 gene and their associations with HCV-related disease risk were investigated in a Saudi Arabian population in this study. Eight TLR3 SNPs were analyzed in 563 patients with HCV, which consisted of 437 patients with chronic HCV infections, 88 with HCV-induced liver cirrhosis, and 38 with HCC. A total of 599 healthy control subjects were recruited to the study. Among the eight TLR3 SNPs studied, the rs78726532 SNP was strongly associated with HCV infection when compared to that in healthy control subjects. The rs5743314 was also strongly associated with HCV-related liver disease progression (cirrhosis and HCC). In summary, these results indicate that distinct genetic variants of TLR3 SNPs are associated with HCV infection and HCV-mediated liver disease progression in the Saudi Arabian population