5 research outputs found
Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare ) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH)
The aim of the present work was to study chromosomal
polymorphism within cultivated barley (Hordeum
vulgare ssp. vulgare) using three-color fluorescence in situ
hybridization (FISH). The physical distribution of the most
frequently used, highly repetitive DNA sequences (GAA)7
specific for pericentromeric heterochromatic regions, the ribosomal
DNA clone pTa71, specific for the 45S rDNA, and
the barley-specific telomere-associated sequence HvT01, was
investigated to reveal genetic diversity in metaphase spreads
of ten barley genotypes with diverse geographical origin,
growth habit and row number. A wild relative of barley,
Hordeum chilense was also studied in order to compare the
polymorphism between and within Hordeum species. Significant
differences in the hybridization patterns of all three DNA
probes could be detected between the two related species, but
only probes pTa71 and HvT01 showed variation in the intensity
and/or position of hybridization sites among genotypes
of H. vulgare ssp. vulgare. The extent of polymorphism
was less than that earlier reported for molecular markers
and was restricted to the long chromosome arms, with
differences between the chromosomes. 1H and 3H proved
to be the most variable chromosomes and 4H and 6H the
most conserved