Chemical composition, antimicrobial activity, proximate analysis and mineral content of the seed of Detarium senegalense JF Gmelin

Detarium senegalense JF Gmelin (Caesalpiniaceae), commonly known as tallow tree, is used traditionally for the treatment of bronchitis, pneumonia, internal complaints and skin diseases in Tropical Africa. The seed is used as a soup thickener in Eastern Nigeria. Analysis of the petroleum ether extract of the seeds with GC-MS produced ten constituents of which oleic and linoleic acids were the most prominent (30.8 and 44.1% respectively). The extract showed significant antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus kristinae, Streptococcus faecalis, Shigella flexneri, Klebsiella pneumonia, Serratia marcescens and antifungal activity against Aspergillus flavus, Aspergillus niger and Penicillium notatum. Proximate analysis revealed that the seeds contain 24.43% carbohydrate, 7.23% protein, 31.16% fiber, 5.89% moisture and 1.93% ash. Mineral content analysis revealed the concentrations of potassium (99.26 mg/g), calcium (71.11mg/g), magnesium (77.83 mg/g), sodium (55.26 mg/g), iron (30.21 mg/g), manganese (7.89 mg/g), zinc (5.26 mg/g) and copper (4.29 mg/g). These results show the nutritional value of the seeds of D. senegalense and justified its use in the traditional treatment of skin diseases.Key words: Detarium, GC-MS, antimicrobial, proximate analysis, mineral content

LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood

<p>Abstract</p> <p>Background</p> <p>Whole-protein extracts from peripheral blood leukocytes are ideal for basic and clinical research. However, lack of a simple preparation technique has limited the use of such extracts. The aim of this study is to develop a simple and easy system that can selectively obtain leukocyte extracts without hemoglobin.</p> <p>Methods</p> <p>A filter that captures the leukocytes but not RBCs was set at the bottom of a 10-mL medical syringe by sandwiching it between plastic stoppers. The capturing efficiency of leukocytes with this tool, called LeukoCatch, was examined using human macrophage cells (MONO-MAC-6). The abilities of LeukoCatch system to capture the leukocyte proteins and to remove the hemoglobin from RBCs were tested by western blot analysis using human blood samples.</p> <p>Results</p> <p>This study presents the development of LeukoCatch, a novel tool that allows the preparation of leukocyte extracts from blood samples within 3 min without centrifugation. Tissue-cultured human macrophage cells were tested to determine the optimal filter numbers and pass-through frequencies of LeukoCatch, which was then applied to 2-mL blood samples. Samples were passed 2~5 times through a LeukoCatch equipped with 5 filters, washed twice with phosphate-buffered saline for red cell removal, and leukocyte proteins were extracted with 0.5 mL of elution buffer. Western blot analysis of the purified extract indicated that more than 90% of hemoglobin was removed by the LeukoCatch and that the protein recovery rate of leukocytes was at least 4 times better than that of the conventional centrifugation method.</p> <p>Conclusion</p> <p>We conclude that LeukoCatch is useful not only for diagnosis at the bedside but also for basic research using blood samples or tissue culture cells.</p

In vitro Anticancer Screening of 24 Locally Used Nigerian Medicinal Plants

Background: Plants that are used as traditional medicine represent a relevant pool for selecting plant candidates that may have anticancer properties. In this study, the ethnomedicinal approach was used to select several medicinal plants native to Nigeria, on the basis of their local or traditional uses. The collected plants were then evaluated for cytoxicity. Methods: The antitumor activity of methanolic extracts obtained from 24 of the selected plants, were evaluated in vitro on five human cancer cell lines. Results: Results obtained from the plants screened indicate that 18 plant extracts of folk medicine exhibited promising cytotoxic activity against human carcinoma cell lines. Erythrophleum suaveolens (Guill. & Perr.) Brenan was found to demonstrate potent anti-cancer activity in this study exhibiting IC50 = 0.2-1.3 $\mu$g/ml. Conclusions: Based on the significantly potent activity of some plants extracts reported here, further studies aimed at mechanism elucidation and bio-guided isolation of active anticancer compounds is currently underway.Chemistry and Chemical Biolog

Chromosomal aberrations induced by Markhamia tomentosa (Benth.) K. Schum. Ex Engl. ethanolic leaf extract on Allium cepa root cells

Markhamia tomentosa (Benth.) K. Schum. Ex Engl. (Bignoniaceae) is used traditionally in the treatment of pain, oedema, pulmonary troubles and cancer. The genotoxic and cytotoxic effects of the ethanolic extract of the leaves of M. tomentosa was investigated using the Allium cepa root chromosomal aberration assay. The mitotic index, chromosomal aberrations and mean root length effects of the plant extract on onion bulbs were determined using 50, 100, 200, 400 and 1000 μg/ml concentrations of the plant extract. Tap water was used as the negative control. The result showed a significant (p&lt;0.05) decrease in mitotic index of the onion root tip cells with increased concentration. The plant extract induced stickiness, c-mitosis, bridges and fragments, vagrant, bi-nucleus, multipolar anaphase and laggard chromosomal aberrations in the tested concentrations with no observable chromosome aberrations in the control group. A significant difference (p&lt;0.05) in the mean root length of onions treated with 400 and 1000 μg/ml concentrations and the control group was recorded. Thus, Markhamia tomentosa ethanol extract exerted mito-depressive, genotoxic and cytotoxic effects on Allium cepa root cells