The Determination of Bio-kinetic Coefficients of Crude Oil Biodegradation Using Pseudomonas Aeruginosa Bacteria

"n "n "nBackgrounds and Objectives: Oil pollution can be generated as a result of spillage, leakage, discharge, exploration, production, refining, transport and storage of crude oil and fuels in the environment. Consequently, many researchers have developed and studied the chemical, physical and biological methods to degrade crude oil. Among them, the biological treatments are the most interesting as they are simple and economical methods. The aim of this study was to determine biokinetic coefficients of crude oil degradation by pseudomonas aerogenusa. This microorganism was isolated in our previous work."nMaterials and Methods: In this study the bio-kinetic coefficients of crude oil biodegradation were evaluated. Pseudomonas aerogenusa bacteria which had been isolated from the soil sample taken from a gas station in our previous work were used in this study. This microorganism was cultured in the liquid medium containing crude oil as sole carbon source. Finally with determining the amount of microorganisms and crude oil concentration during biodegradation process, the bio-kinetic coefficients based on modified Monod equation were calculated."nResults: bio-kinetic coefficients obtained from laboratory studies are vital factors in industrial applications. As a result, the bio-kinetic study was performed to find bio-kinetic coefficients for biodegradation of crude oil using the isolated bacteria. The results showed that ,Y, k and were equal 0.107 , 0.882 , 9.39 and 169.3 respectively."nCoculusion:Our results showed that pseudomonas aerogenusa is usable for treatment of oily wastewaters in the full scale facility. Results of this study indicated bio kinetics confections

Effect of Gibberellic Acid under Deficit Irrigation on Physicochemical and Shelf Life Attributes of Pomegranate Fruit (cv. Shahvar)

Introduction: Pomegranate (Punica granatum L.) belonging to the family Punicaceae, native to subtropical regions of Iran and adapted to arid or semi arid climates with mild winters. Pomegranate is fairly drought tolerant but requires regular irrigation to produce high yield and fruit weight. Large parts of Iran within the boundaries of central deserts (Dasht-e-kavir and Kavir-e-Loot) have arid or semi-arid conditions which make them suitable for pomegranate production. However drought crisis and water resources restriction are very serious in these areas. Materials and Methods: This experiment was conducted on 7-year old pomegranate cv. Shahvar trees from 2013 to 2014 in Torbat-e-Heydarieh, Razavi Khorasan, Iran. Irrigation treatments and Gibberellic acid application were used in Completely Randomized Split-Plot Design with four replications. Irrigation treatments included [1-control: 100% of estimated crop evapotranspiration (Etc) 2-Sustained deficit irrigation (SDI): watering was constantly used at 50%Etc, and 3-Regulated deficit irrigation (RDI): not watering was imposed until fruit set and then irrigation was applied same as control]. Foliar application of Gibberellic acid was done with two concentrations (0 and 150 ppm) at early May and September. Precipitation and pan evaporation (Ep) was recorded by weather station that located at 15 km distance from the studied orchard. Daily crop reference evapotranspiration (ETo) was estimated by penman-monteith equation. Trees were drip-irrigated by two lateral lines parallel to the tree row and four emitters that each one delivers 4 liters per hour. Fruit weight and numbers, tree production (yield), peel, arils and juice percent and finally fruit cracking of each treatment were determined at ordinary harvest time in late of October. Some uniform and intact fruits per treatment transferred to cold storage (T= 5 ◦C, RH= 85-90%). After storage period the fruits transfered to shelf life condition (7 days at 20 ◦C and RH= 65-70%) to evaluate physiochemical traits at 2 different storage periods (9 and 18 weeks). Weight loss and chilling index were determined during 3 weeks intervals of storage. Weight loss was evaluated by a gravimetric method and results were expressed as percent of initial fresh weight. Chilling index was quantified by 5 point scale of fruit husk injury: (1: without disorder, 2: slight disorder signs 3: moderate signs 4: severe signs and 5: unmarketable). Results and Discussion: In present research the effects of deficit irrigation treatments on all the measured attributes was significant at harvest time (p≤ 0.01). But Gibberellic acid spray had significant effect only on Fruit weight, juice percent and fruit cracking disorder. Likewise, deficit irrigation and Gibberellic acid interaction showed significant difference for fruit weight and cracking disorder. The results indicated that fruit weight, total yield and fruit juice in regulated deficit irrigation increased by 39.6, 17.1 and 16.6 percent in compare with control, respectively. Fruit numbers in control trees (108.3) was more than sustained (93.6) and regulated (87) deficit irrigation. It is possible that sustained (SDI) and regulated (RDI) deficit irrigation have decreased sprouting growth and consequently second or third waves of pomegranate flowers that forming on these shoots was lower by water restricting. Pomegranate peel percent in studied deficit irrigation strategies were less than control and naturally aril percent was more in these treatments. In other hand, the highest fruit cracking (9.1%) and lowest fruit weight (205.8 g) occurred in sustained deficit irrigation. However Gibberellic acid application could increase fruit weight and alleviate cracking disorder. The results of cold storage experiment showed that maturity index, antioxidant activity, total anthocyanin and chilling index improved by Sustained deficit irrigation. Variations of ripening index during cold storage occurred because of sugar conversion, not the changes of organic acids content. Indeed, rate of starch degradation to simple carbohydrates in fruits increase under drought condition. Probability drought in deficit irrigation treatments as an oxidative stress motivates antioxidant system and consequently increases chilling resistance in pomegranate fruits. Preharvest Gibberellic acid application amended weight loss and increased total anthocyanin and antioxidant activity during cold storage. Conclusions: On the base of this study it seems that using of some deficit irrigation strategies have acceptable consequences on pomegranate fruit production at conditions of water resources restriction. Likewise Gibberellic acid application on trees that were subjected by deficit irrigations ameliorates the adverse effects of drought stress

Biodegradation of MTBE by Bacteria Isolated from oil Hydrocarbons-Contaminated Environments

Methyl tertiary butyl ether (MTBE) belongs to the group of gasoline oxygenates and persistent environment contaminants, and shows potential for biodegradation in aerobic and anaerobic conditions, through application of pure microbial cultures. Presented research shows that indigenous bacterial isolates 6sy and 24p, selected from oil hydrocarbons-contaminated environments, were capable of utilizing MTBE as sole carbon and energy source. Based on 16S rDNA sequence analysis, bacterial isolates 6sy and 24p were identified as Staphylococcus saprophyticus subsp. saprophyticus and Pseudomonas sp., respectively. The MTBE biodegradation rate was affected by longevity of incubation period and initial MTBE concentration. After 3 weeks of incubation at 25 degrees C in a dark, the removal rates of initial 25 and 125 ppm MTBE concentrations by Staphylococcus saprophyticus 6sy were found to be 97, and 63%, respectively, while efficiency of Pseudomonas sp. in degradation of indicated concentrations was 96, and 40%, respectively. Both bacterial isolates were able to grow in MTBE-containing growth medium. Highest growth rate of bacterial isolates was observed at the end of incubation period. The presented results indicated the potential of these bacterial isolates in bioremediation of MTBE-contaminated environments