The Role of Anti-dengue Virus NS-1 and Anti-protein Disulfide Isomerase Antibodies on Platelet Aggregation in Secondary Dengue Infection

Aim: to observe the correlation between anti-NS-1 and anti PDI antibodies against platelets function disorder on secondary dengue infection. Methods: 50 patients with secondary DV infection according to WHO criteria were observed by a cross sectional study. Patient’s blood was collected on day 3, 5 and 7 after fever onset. Platelets aggregation test was done to prove the possibility of platelets dysfunction. Anti-NS-1 and anti- PDI antibodies were determined by solid phase ELISA. Results: the inhibition of platelets aggregation was increased among day of observation. Means value of inhibition on day 3 is 46.6%, day 5 is 52.5% and day 7 is 56%. There is a significant difference (p0.05). Conclusion: the kinetics profile of NS1 and PDI antibodies responses, which were detected by the third day of symptoms. Dengue patients’ sera inhibited platelets aggregation. NS-1 antibodies and PDI antibodies might have a role on the platelets aggregation dysfunction; however, there is no correlation between them. It is possible that other mechanism involve in the inhibition of platelets aggregation.Key words: anti-NS-1 antibodies, anti PDI antibodies, inhibition platelets aggregation, secondary dengue infection

The effect of make a match technique on students’ reading comprehension at XI grade of MAN 1 Padangsidimpuan

This research focused on the effect of Make a Match technique on students’ reading comprehension at XI grade of MAN 1 Padangsidimpuan. The students’ problems in reading comprehension were: 1) Most of the students still get low grade with averages was 60. 2) Most of students did not understand the text that the students read. They just read the text without comprehending the text. 3) Most of students easy to feel bored when they learnt about the English text. Beside the students’ problem, teacher’s technique also became a problem in learning English. The teacher still used the conventional teaching and did not have variation in teaching reading. The purpose of this research was to know whether there is the significant effect of Make a Match technique on students’ reading comprehension at XI grade of MAN 1 Padangsidimpuan. The method used in this research was experimental research. Two classes were chosen randomly as the sample. They were XI MIA 3 as experimental class that consisted of 40 students and XI MIA 4 as control class that consisted of 40 students. It was taken after conducting normality and homogeneity test. The data was derived from pre-test and post-test. To analyze the data, the researcher used t-test formula. After analyzing the data, the researcher found that mean score of experimental class after using Make a Match technique was higher than control class. Mean score of experimental class before using Make a Match technique was 64.13 and mean score after using Make a Match technique was 75.23 and it had gain 11.10. The effect of Make a Match technique on students’ reading comprehension was 3.009 with tcount was higher than ttable (3.009 > 2.000). It meant that Ha was accepted and H0 was rejected. It was concluded that there was a significant effect of Make a Match technique on students’ reading comprehension at XI grade of MAN 1 Padangsidimpuan