Peptide mimics selected from immune sera using phage display technology can replace native antigens in the diagnosis of Epstein–Barr virus infection

There is an expanding area of small molecule discovery, especially in the area of peptide mimetics. Peptide sequences can be used to substitute for the entire native antigen for use in diagnostic assays. Our approach is to select peptides that mimic epitopes of the natural immune response to Epstein–Barr virus (EBV) that may be recognised by antibodies typically produced after infection with EBV. We screened a random peptide library on sera from rabbits immunised with a crude preparation of EBV and serum antibodies from a patient with a high titer of EBV antibodies. We selected four peptides (Eb1–4) with the highest relative binding affinity with immune rabbit sera and a single peptide with high affinity to human serum antibodies. The peptides were coupled to the carrier molecule BSA and the recognition of the peptides by IgM antibodies in clinical samples after infection with EBV was measured. The sensitivities were Eb1 94%, Eb2, 3, 4 88%, H1 81% and all had 100% specificity. This study illustrates that the phage display approach to select epitope mimics can be applied to polyclonal antibodies and peptides that represent several diagnostically important epitopes can be selected simultaneously. This panel of EBV peptides representing a wide coverage of immunodominant epitopes could replace crude antigen preparations currently used for capture in commercial diagnostic tests for EBV

Qualitative Properties of Magnetic Fields in Scalar Field Cosmology

We study the qualitative properties of the class of spatially homogeneous Bianchi VI_o cosmological models containing a perfect fluid with a linear equation of state, a scalar field with an exponential potential and a uniform cosmic magnetic field, using dynamical systems techniques. We find that all models evolve away from an expanding massless scalar field model in which the matter and the magnetic field are negligible dynamically. We also find that for a particular range of parameter values the models evolve towards the usual power-law inflationary model (with no magnetic field) and, furthermore, we conclude that inflation is not fundamentally affected by the presence of a uniform primordial magnetic field. We investigate the physical properties of the Bianchi I magnetic field models in some detail.Comment: 12 pages, 2 figures in REVTeX format. to appear in Phys. Rev.

About Bianchi I with VSL

In this paper we study how to attack, through different techniques, a perfect fluid Bianchi I model with variable G,c and Lambda, but taking into account the effects of a $c$-variable into the curvature tensor. We study the model under the assumption,div(T)=0. These tactics are: Lie groups method (LM), imposing a particular symmetry, self-similarity (SS), matter collineations (MC) and kinematical self-similarity (KSS). We compare both tactics since they are quite similar (symmetry principles). We arrive to the conclusion that the LM is too restrictive and brings us to get only the flat FRW solution. The SS, MC and KSS approaches bring us to obtain all the quantities depending on \int c(t)dt. Therefore, in order to study their behavior we impose some physical restrictions like for example the condition q<0 (accelerating universe). In this way we find that $c$ is a growing time function and Lambda is a decreasing time function whose sing depends on the equation of state, w, while the exponents of the scale factor must satisfy the conditions $\sum_{i=1}^{3}\alpha_{i}=1$ and $\sum_{i=1}^{3}\alpha_{i}^{2}<1,$ $\forall\omega$, i.e. for all equation of state$,$ relaxing in this way the Kasner conditions. The behavior of $G$ depends on two parameters, the equation of state $\omega$ and $\epsilon,$ a parameter that controls the behavior of $c(t),$ therefore $G$ may be growing or decreasing.We also show that through the Lie method, there is no difference between to study the field equations under the assumption of a $c-$var affecting to the curvature tensor which the other one where it is not considered such effects.Nevertheless, it is essential to consider such effects in the cases studied under the SS, MC, and KSS hypotheses.Comment: 29 pages, Revtex4, Accepted for publication in Astrophysics & Space Scienc

Light propagation in statistically homogeneous and isotropic universes with general matter content

We derive the relationship of the redshift and the angular diameter distance to the average expansion rate for universes which are statistically homogeneous and isotropic and where the distribution evolves slowly, but which have otherwise arbitrary geometry and matter content. The relevant average expansion rate is selected by the observable redshift and the assumed symmetry properties of the spacetime. We show why light deflection and shear remain small. We write down the evolution equations for the average expansion rate and discuss the validity of the dust approximation.Comment: 42 pages, no figures. v2: Corrected one detail about the angular diameter distance and two typos. No change in result

Isolation of antibodies specific to a single conformation-dependant antigenic determinant on the EG95 hydatid vaccine

EG95 is a recombinant vaccine protein that elicits protection against hydatid disease in sheep. Previous studies have shown that the host-protective epitopes on EG95 depend on correct conformation and cannot be represented by simple “linear” peptides. By screening random peptide phage display libraries with polyclonal antibodies directed against conformation-dependant epitopes of EG95, we have selected a number of peptides that mimic these epitopes. The selected peptides did not show sequence homology to EG95. Antigen binding assays involving these peptides have provided evidence of at least four conformationally-dependant epitope regions on EG95. One of the selected peptides, E100, has been used to purify antibodies from anti-sera raised in sheep vaccinated with EG95. This yielded monospecific antibodies capable of recognizing recombinant EG95 in ELISA and native EG95 in Western blot assays. This antibody was demonstrated to be effective in antibody-dependant complement-mediated in vitro killing of Echinococcus granulosus oncospheres. Peptide E100 may represent the basis for a quality control assay for EG95 production, and has the potential to become a component of a synthetic peptide-based vaccine against E. granulosus

A radar survey of M- and X-class asteroids. III. Insights into their composition, hydration state, & structure

Using the S-band radar at Arecibo Observatory, we observed thirteen X/M-class asteroids; nine were previously undetected and four were re-observed, bringing the total number of Tholen X/M-class asteroids observed with radar to 29. Of these 29M-class asteroids, 13 are also W-class, defined as M-class objects that also display a 3-μm absorption feature which is often interpreted as the signature of hydrated minerals (Jones, T.D., Lebofsky, L.A., Lewis, J.S., Marley, M.S. [1990]. Icarus 88, 172-192; Rivkin, A.S., Howell, E.S., Britt, D.T., Lebofsky, L.A., Nolan, M.C., Branston, D.D. [1995]. Icarus 117, 90-100; Rivkin, A.S., Howell, E.S., Lebofsky, L.A., Clark, B.E., Britt, D.T. [2000]. Icarus 145, 351-368).Consistent with our previous work (Shepard, M.K. et al. [2008]. Icarus 195, 184-205; Shepard, M.K., Harris, A.W., Taylor, P.A., Clark, B.E., Ockert-Bell, M., Nolan, M.C., Howell, E.S., Magri, C., Giorgini, J.D., Benner, L.A.M. [2011]. Icarus 215, 547-551), we find that 38% of our sample (11 of 29) have radar albedos consistent with metal-dominated compositions. With the exception of 83 Beatrix and 572 Rebekka, the remaining objects have radar albedos significantly higher than the mean S- or C-class asteroid (Magri, C., Nolan, M.C., Ostro, S.J., Giorgini, J.D. [2007]. Icarus 186, 126-151).Seven of the eleven high-radar-albedo asteroids, or 64%, also display a 3-μm absorption feature (W-class) which is thought to be inconsistent with the formation of a metal dominated asteroid. We suggest that the hydration absorption could be a secondary feature caused by low-velocity collisions with hydrated asteroids, such as CI or CM analogs, and subsequent implantation of the hydrated minerals into the upper regolith. There is recent evidence for this process on Vesta (Reddy, V. et al. [2012]. Icarus 221, 544-559; McCord, T.B. et al. [2012]. Nature 491, 83-86; Prettyman, T.H. et al. [2012]. Science 338, 242-246; Denevi, B.W. et al. [2012]. Science 338, 246-249).Eleven members of our sample show bifurcated radar echoes at some rotation phases; eight of these are high radar albedo targets. One interpretation of a bifurcated echo is a contact binary, like 216 Kleopatra, and several of our sample are contact binary candidates. However, evidence for other targets indicates they are not contact binaries. Instead, we hypothesize that these asteroids may have large-scale variations in surface bulk density, i.e. isolated patches of metal-rich and silicate-rich regions at the near-surface, possibly the result of collisions between metal and silicate-rich asteroids