Posterior urethral valve: a retrospective review of cases and challenges of management in a low-resource centre

Objective: Posterior urethral valves (PUV) diagnosis and management remain a major challenge in developing countries. We sought to review cases of PUV managed in our health centre and to highlight the challenges in its management.Methods: A retrospective analysis of patients with PUV managed over a 12month period. Data obtained from patients' medical records include: demography, clinical presentation, serum electrolytes, urea and creatinine, radiological diagnosis, initial management, surgical management and the outcome of management.Results: Eight patients were managed during the period under review. The median age was 26 months. The commonest clinical feature was recurrent fever. Three patients (37.5%) had renal insufficiency requiring peritoneal dialysis at initial presentation. Six patients (75%) had Mohan's valvotomy while two (25%) died before valve ablation. One patient (12.5%) died a month after valve ablation from renal failure, while one (12.5%) had residual valve requiring a repeat valve ablation. Post-operative complication of urosepsis occurred in two patients (25%).Conclusions: Physicians should have a high index of suspicion for PUV in boys who present with recurrent fever or urinary tract infections. Screening for foetal anomalies during routine ante-natal maternal ultrasonography will improve prenatal diagnosis of this condition.Keywords: Low-resource centre, Management, Nigeria, Posterior urethral valv

Caveolin expression changes in the neurovascular unit after juvenile traumatic brain injury: signs of blood-brain barrier healing?

Traumatic brain injury (TBI) is one of the major causes of death and disability in pediatrics, and results in a complex cascade of events including the disruption of the blood-brain barrier (BBB). A controlled-cortical impact on post-natal 17-day-old rats induced BBB disruption by IgG extravasation from 1 to 3 days after injury and returned to normal at day 7. In parallel, we characterized the expression of three caveolin isoforms, caveolin 1 (cav-1), caveolin 2 (cav-2) and caveolin 3 (cav-3). While cav-1 and cav-2 are expressed on endothelial cells, both cav-1 and cav-3 were found to be present on reactive astrocytes, in vivo and in vitro. Following TBI, cav-1 expression was increased in blood vessels at 1 and 7 days in the perilesional cortex. An increase of vascular cav-2 expression was observed 7 days after TBI. In contrast, astrocytic cav-3 expression decreased 3 and 7 days after TBI. Activation of endothelial nitric oxide synthase (eNOS) (via its phosphorylation) was detected 1 day after TBI and phospho-eNOS was detected both in association with blood vessels and with astrocytes. The molecular changes involving caveolins occurring in endothelial cells following juvenile-TBI might participate, independently of eNOS activation, to a mechanism of BBB repair while, they might subserve other undefined roles in astrocytes

Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

The aim of this study was to evaluate the viability of bull spermatozoa diluted with commercial semen extender and two culture media stored at controlled room temperature (24 °C) for 72 hours. Two Nguni bulls were used for semen collection with the aid of an electro-ejaculator. After macroscopic evaluation, semen was pooled and aliquoted randomly into Triladyl, modified Ham’s F10, and TCM-199 culture media, and then stored at 24 °C. Sperm motility parameters, morphology, and viability were analysed with computer aided sperm analysis (CASA) after 0, 24, 48 and 72 hours. The study was replicated four times, and data were analysed using analysis of variance (ANOVA). Triladyl had significantly higher sperm viability rate (41.3%) and total motility rate (96.3%) for 72 hours than modified Ham’s F10 (86.8%; 26.5%) and TCM-199 (76.7%; 25.0%) culture media. Ham’s F10 had higher progressive motility rate (37.8%) than the other extenders TCM-199 (31.7%) and Triladyl (23.4%). There was no significant difference in viability rate between Ham’s F10 (26.5%) and TCM-199 (25.0 %) after 72 hours’ storage at 24 °C. Furthermore, no significant difference was observed in total sperm abnormalities, except for reacted acrosomes and absent tails, between the two Nguni bulls. Nguni semen can be preserved in Triladyl or modified Ham’s F10 and TCM-199 culture media, stored at 24 °C, and stay viable for 72 hours.Keywords: Ham’s F10, TCM-199, Triladyl, sperm motility, viabilit

Activation of pro-apoptotic cells, reactive astrogliosis and hyperphosphorylation of tau protein in trimethyltin-induced hippocampal injury in rats

Neurodegenerative diseases cause neural cells to lose both the functional and sensory abilities as a result of genetic factors, proteopathies and mitochondrial dysfunction. Neurodegeneration forms the basis of most neurodegenerative disorders for example Alzheimer’s disease, Huntington’s diseases, and Parkinson’s diseases. The mechanism that underlines the process of neurodegeneration is not well understood. Understanding the process and mechanism involved in neurodegeneration might offer a better therapeutic approach to positively manage cases of neurodegenerative diseases. Therefore, this study’s target was to create an animal model to study neurodegeneration. Sixteen adult male Wistar rats were used in the study and divided into two groups. Control (0.2 mL of normal saline (NS)), and trimethyltin-treated (TMT, 8 mg/kg stat dose only). These animals underwent perfusion with 4% paraformaldehyde, brain excision and analysis of p53 antigen, GFAP and Bielshowsky on these tissues. The results showed that animals in the control group showed presence of activated p53 antigen, reactive astrogliosis, neurofibrillary tangles, and amyloid plaques within the cytoplasm of the hippocampal cells. Cornus Ammonis (CA2) and (CA3) showed more of the trimethylrtin injury than CA1 and CA4. This study thus revealed that, intra-peritoneal administration of single dose of 8mg/kg of trimethyltin can offer an attractive disease model to study some neurodegenerative diseases. Keywords: p53 antigen, Bielshowsky, Glia fibrillary acidic protein, Trimethyltin, Hippocampus

Effect of varying level of ginger powder in the diet of starter turkeys on performance, haematological and serum biochemical indices

Dietary inclusion of various phytogenic additives has been used as alternative growth promoters in poultry. They enhance performance and stabilize health status of farm animals. This study was conducted to investigate the effect of inclusion of ginger powder to the diets of pre starter and starter turkeys at different levels (0g/kg, 2g/kg and 4g/kg) on its performance and some blood parameters. A total of one hundred and twenty day- old British United Turkey (BUT) poults were allotted on weight equalization basis into the 3 dietary treatments in a complete randomised design. Forty poults were assigned to each treatment group replicated four times with 10 poults per replicate. The study lasted for 56 days comprising of the prestarter (0-28 days) and starter (29-56 days) phases. At the pre-starter phase of the experiment, growth performance was not affected (p>0.05) by ginger powder inclusion while the starter phase showed improved (p<0.05) final weight, feed intake and feed conversion ratio. Final weight increased (p<0.05) with ginger inclusion in the diets, while feed intake reduced linearly as ginger inclusion increased. Supplementation with ginger reduced haemoglobin and RBC of the pre starter turkeys. The RBC increases linearly (p<0.05) as ginger supplementation increases with starter turkeys. Serum albumin improved significantly (p<0.05) following dietary inclusion of 2 g/kg ginger powder at the starter (3.37 g/dl) phase. In conclusion, starter turkeys fed diet containing ginger at 2 g/kg showed improved growth with improvement in hepatic enzyme activities