Immunisation against gonadotrophin-releasing hormone (GnRH) reduces agonistic behaviours in male rangeland goats

Rangeland goat bucks were used to evaluate the efficacy of a commercially available anti-gonadotrophin-releasing hormone vaccine, Improvac (Zoetis Australia, West Ryde, NSW, Australia). The hypothesis tested was that immunisation would suppress testosterone secretion by the testis and agonistic behaviour between male goats. We also compared intervals of 2 and 4 weeks between primary and booster immunisations and monitored responses over a 2-month period. The 45 goats were split into three groups (n = 15): one group receiving the vaccination booster on Day 14, one group receiving the vaccination booster on Day 28, and the Control group receiving sterile saline injections. Body mass, body condition score and scrotal circumference were measured fortnightly, and blood was collected at 2-week intervals and analysed for testosterone concentration. Behavioural interaction tests of 2-min duration were also conducted fortnightly. There was a significant decrease in paired testicular circumference (P < 0.05) and testosterone concentration (P < 0.01) in both vaccination groups by the end of the experiment at Day 60, compared with the Control group. Agonistic interactions measured at Day 60 were significantly reduced in both vaccination groups (P < 0.05) compared with the Control group. These results support the efficacy of Improvac in reducing agonistic behaviours in rangeland goat bucks and suggest that the use of the vaccine may assist in reducing social stress and possible injury in groups of confined male goats

Animal welfare and the use of procedural documents: Limitations and refinement

Increased scrutiny of animal welfare in wildlife management has seen a recent proliferation in the use of procedural documents (standard operating procedures, codes of practice etc.). Some procedural documents are presumed to represent 'best practice' methods, whereby adherence to prescribed inputs is explicitly purported to generate humane outcomes. However, the relationship between what is done to animals (inputs) and what they experience (outputs), as assessed by animal-based measures, has received little attention. Procedural documents are commonly developed in the absence of empirical animal-based measures, creating uncertainty in animal welfare outcomes. Prescribed procedures are valuable as guidelines for standardising methodology, but the development of 'welfare standards' that focus on desired thresholds for animal-based measures offers many advantages for improving animal welfare. Refinement of the use of procedural documents in wildlife management is required to ensure they generate desirable outcomes for animals, and do not preclude the development of improved methods

Animal welfare indicators for sheep during sea transport: The effect of voyage day and time of day

Ensuring the well-being of animals during transport is becoming an increasingly important societal concern. The Australian livestock export industry recognises the need for comprehensive monitoring and reporting on animal welfare during sea transport. It is predicted that pen-side assessments of sheep can be used to monitor environmental conditions, resource access, and animal health and behavioural outcomes throughout a sea voyage. Pen-side assessments by observation are non-invasive and practical to apply in an industry setting. This study monitored sheep using a pilot list of welfare indicators during two sea voyages from Australia to the Middle East, in contrasting seasons. Sheep behaviour, environment and resources were recorded three times daily via pen-side observations of six pens of Merino wethers (castrated males), repeated over three decks for each voyage. Behavioural outcomes were examined for the effect of sampling frequency on group assessments. The number of behavioural measures were reduced via Principal Component (PC) analysis. The primary three PC factors were tested against the time of sampling and pen location after accounting for the effect of environmental- and resource-based predictor variables. PC 1 (24.0 % of the total variance) described activity levels, with sheep on Voyage B being more active in the morning and resting or recumbent in the middle of the afternoon and evening. PC 2 (14.7 %) reflected heat responses with the majority of the variation in these data accounted for by changes in Wet Bulb Globe Temperature (WBGT) and manure pad moisture. The heat responses described by PC 2 also varied by voyage day (p < 0.001) and time point (p < 0.001). PC 3 scores (9.5 %) reflected flight distances and feeding behaviour and strongly correlated to WBGT and pellet consumption per head per day. Feeding behaviour generally became more competitive, and flight distances reduced as both voyages progressed. Results indicate that a comprehensive welfare monitoring protocol requires repeated daily sampling throughout a voyage. The findings of this study are pertinent for developing a sampling strategy to assess sheep welfare during sea transport

The contribution of qualitative behavioural assessment to appraisal of livestock welfare

Animal welfare is increasingly important for the Australian livestock industries, to maintain social licence to practice as well as ensuring market share overseas. Improvement of animal welfare in the livestock industries requires several important key steps. Paramount among these, objective measures are needed for welfare assessment that will enable comparison and contrast of welfare implications of husbandry procedures or housing options. Such measures need to be versatile (can be applied under a wide range of on- and off-farm situations), relevant (reveal aspects of the animal’s affective or physiological state that is relevant to their welfare), reliable (can be repeated with confidence in the results), relatively economic to apply, and they need to have broad acceptance by all stakeholders. Qualitative Behavioural Assessment (QBA) is an integrated measure that characterises behaviour as a dynamic, expressive body language. QBA is a versatile tool requiring little specialist equipment suiting application to in situ assessments that enables comparative, hypothesis-driven evaluation of various industry-relevant practices. QBA is being increasingly used as part of animal welfare assessments in Europe, and although most other welfare assessment methods record ‘problems’ (e.g. lameness, injury scores, and so on), QBA can capture positive aspects of animal welfare (e.g. positively engaged with their environment, playfulness). In this viewpoint, we review the outcomes of recent QBA studies and discuss the potential application of QBA, in combination with other methods, as a welfare assessment tool for the Australian livestock industries

The changing trend in nitrate concentrations in major aquifers due to historical nitrate loading from agricultural land across England and Wales from 1925 to 2150

Nitrate is necessary for agricultural productivity, but can cause considerable problems if released into aquatic systems. Agricultural land is the major source of nitrates in UK groundwater. Due to the long time-lag in the groundwater system, it could take decades for leached nitrate from the soil to discharge into freshwaters. However, this nitrate time-lag has rarely been considered in environmental water management. Against this background, this paper presents an approach to modelling groundwater nitrate at the national scale, to simulate the impacts of historical nitrate loading from agricultural land on the evolution of groundwater nitrate concentrations. An additional process-based component was constructed for the saturated zone of significant aquifers in England and Wales. This uses a simple flow model which requires modelled recharge values, together with published aquifer properties and thickness data. A spatially distributed and temporally variable nitrate input function was also introduced. The sensitivity of parameters was analysed using Monte Carlo simulations. The model was calibrated using national nitrate monitoring data. Time series of annual average nitrate concentrations along with annual spatially distributed nitrate concentration maps from 1925 to 2150 were generated for 28 selected aquifer zones. The results show that 16 aquifer zones have an increasing trend in nitrate concentration, while average nitrate concentrations in the remaining 12 are declining. The results are also indicative of the trend in the flux of groundwater nitrate entering rivers through baseflow. The model thus enables the magnitude and timescale of groundwater nitrate response to be factored into source apportionment tools and to be taken into account alongside current planning of land-management options for reducing nitrate losses

The fine sediment conundrum; quantifying, mitigating and managing the issues

Copyright © 2017 John Wiley & Sons, Ltd. Excess fine sediment is a global cause of lotic ecosystem degradation. Despite historic interest in identifying sediment sources and quantifying instream dynamics, tackling fine sediment problems remains a key challenge for river managers and a continued focus of international research. Accordingly, a national meeting of the British Hydrological Society brought together those working on fine sediment issues at the interface of hydrology, geomorphology, and ecology. The resulting collection of papers illustrates the range of research being undertaken in this interdisciplinary research arena, by academic researchers, environmental regulators, landowners, and consultants. More specifically, the contributions highlight key methodological advancements in the identification of fine sediment sources, discuss the complexities surrounding the accurate quantification of riverbed fine sediment content, demonstrate the potential utility of faunal traits as a biological monitoring tool, and recognize the need for improved mechanistic understanding of the functional responses of riverine organisms to excess fine sediment. Understanding and mitigating the effects of fine sediment pressures remains an important and multifaceted problem that requires interdisciplinary collaborative research to deliver novel and robust management tools and sustainable solutions

Review of livestock welfare indicators relevant for the Australian live export industry

Animal welfare is an important issue for the live export industry (LEI), in terms of economic returns, community attitudes and international socio-political relations. Mortality has traditionally been the main welfare measure recorded within the LEI; however, high mortality incidents are usually acted upon after adverse events occur, reducing the scope for proactive welfare enhancement. We reviewed 71 potential animal welfare measures, identifying those measures that would be appropriate for use throughout the LEI for feeder and slaughter livestock species, and categorised these as animal-, environment- and resource-based. We divided the live export supply chain into three sectors: (1) Australian facilities, (2) vessel and (3) destination country facilities. After reviewing the relevant regulations for each sector of the industry, we identified 38 (sector 1), 35 (sector 2) and 26 (sector 3) measures already being collected under current practice. These could be used to form a ‘welfare information dashboard’: a LEI-specific online interface for collecting data that could contribute towards standardised industry reporting. We identified another 20, 25 and 28 measures that are relevant to each LEI sector (sectors 1, 2, 3, respectively), and that could be developed and integrated into a benchmarking system in the future

Interactions between fine-grained sediment delivery, river bed deposition and salmonid spawning success

Salmonids clean river bed gravels to lay their eggs. However, during the incubation period fine sediment infiltrates the bed. This has been found to limit the success of salmonid spawning, as fine sediment reduces gravel permeability resulting in intra-gravel flow velocities and O2 concentrations decreasing. The success of salmonid spawning is therefore a function of the coincidence of fine sediment delivery and the development of the salmonid eggs. The presence of fine sediment also exerts sub-lethal effects on the rate of egg development with a negative feedback slowing and extending the incubation process meaning the eggs are in the gravels for longer and susceptible to more potential sediment delivery events. The SIDO (Sediment Intrusion and Dissolved Oxygen)- UK model is a physically-based numerical model which simulates the effect of fine sediment deposition on the abiotic characteristics of the salmonid redd, along with the consequences for egg development and survival. This model is used to investigate the interactions and feedbacks between the timing and concentrations of suspended sediment delivery events, and the deposition of fine sediment within the gravel bed, and the consequences of this on the rate of egg development and survival. The model simulations suggest that egg survival is highly sensitive to suspended sediment concentrations, particularly to changes in the supply rate of sand particles. The magnitude, frequency and specific timing of sediment delivery events effects egg survival rates. The modelling framework is also used to investigate the impact of the rate of gravel infilling by sediment. The hypotheses of continual, discrete event and non-linear decline in the rate of infilling are investigated

Stochastic Resonance in Ion Channels Characterized by Information Theory

We identify a unifying measure for stochastic resonance (SR) in voltage dependent ion channels which comprises periodic (conventional), aperiodic and nonstationary SR. Within a simplest setting, the gating dynamics is governed by two-state conductance fluctuations, which switch at random time points between two values. The corresponding continuous time point process is analyzed by virtue of information theory. In pursuing this goal we evaluate for our dynamics the tau-information, the mutual information and the rate of information gain. As a main result we find an analytical formula for the rate of information gain that solely involves the probability of the two channel states and their noise averaged rates. For small voltage signals it simplifies to a handy expression. Our findings are applied to study SR in a potassium channel. We find that SR occurs only when the closed state is predominantly dwelled. Upon increasing the probability for the open channel state the application of an extra dose of noise monotonically deteriorates the rate of information gain, i.e., no SR behavior occurs.Comment: 10 pages, 2 figures, to appear in Phys. Rev.