13 research outputs found
Comparison of Arabidopsis thaliana (col-0) plants and cell suspension cultures responses to Alternaria brassicicola infection
Transcription profiling of Arabidopsis thaliana and Brassica oleracea var. capitata f. alba during Alternaria brassicicola infection reveals different mode of action of fungus
Green islands develop in a leaf age-dependent manner during black spot disease of Brassica juncea
Efficient in vitro callus induction and regeneration of different Polish cultivars of tomato
Brassica oleracea var. botrytis: evaluation of in vitro potential of two commercial cultivars
New Vistas are Opened for Sorghum Improvement by Genetic Transformation
Transgenic fertile sorghum plants (Sorghum bicolor L. Moench) were
obtained by microprojectile bombardment of immature embryo and immature
inflorescence explants. Regeneration from the calli was through
embryogenesis and organogenesis pathways, resulting in both the
uniformly transformed and chimeric plants. Plant regeneration occurred
on media supplemented with bialaphos. Microprojectile bombardment
resulted in plants resistant to the herbicide and exhibited
phosphinothricin acetyltransferase activity. The presence of bar, uMa
and luc genes in the T[o] plants was confirmed by Southern blot
analysis of genomic DNA. The herbicide resistance was inherited in T[1]
plants.Les plantes transgeniques fertiles de surgho (Sorghum bicolor L. Moench
) etaient obtenues par bombardement de microprojectiles d'embryons
immatures et d'explants d'inflorescences immatures. La regeneration a
partit de cales a lieu a travers l'embryogenese et l'organogenese qui
resultent en la formation des plants uniformement transformes et des
chimeres. La regeneration des plantes se fait sur des milieux ayant
obtenu un supplement de bialaphos. Le bombardement des microprojectiles
a abouti a la production des plants resistants aux herbicides et
presentant l'activite phosphinothricine acetyltransferase. La presence
des genes bar, uida et luc sur les plants T[o] a ete confirmee a l'aide
de l'analyse southern blot du DNA genomique. La resistance a
l'herbicide etait herite par les plants en T[1]