A critical analysis of high-redshift, massive galaxy clusters: I

We critically investigate current statistical tests applied to high redshift clusters of galaxies in order to test the standard cosmological model and describe their range of validity. We carefully compare a sample of high-redshift, massive, galaxy clusters with realistic Poisson sample simulations of the theoretical mass function, which include the effect of Eddington bias. We compare the observations and simulations using the following statistical tests: the distributions of ensemble and individual existence probabilities (in the >M,>z sense), the redshift distributions, and the 2d Kolmogorov-Smirnov test. Using seemingly rare clusters from Hoyle et al. (2011), and Jee et al. (2011) and assuming the same survey geometry as in Jee et al. (2011, which is less conservative than Hoyle et al. 2011), we find that the (>M,>z) existence probabilities of all clusters are fully consistent with LCDM. However assuming the same survey geometry, we use the 2d K-S test probability to show that the observed clusters are not consistent with being the least probable clusters from simulations at >95% confidence, and are also not consistent with being a random selection of clusters, which may be caused by the non-trivial selection function and survey geometry. Tension can be removed if we examine only a X-ray selected sub sample, with simulations performed assuming a modified survey geometry.Comment: 20 pages, 6 figures, 2 tables, modified to match accepted version (JCAP); title changed, main analysis unchanged, additional analysi

Street canyon ventilation: Combined effect of cross‐section geometry and wall heating

Understanding the dynamics ofmass exchange between a street canyon and the overlying atmosphere is crucial to predict air quality in urban areas. Despite the large number of studies on this topic, there are many aspects that still need to be clarified. Among these, one is certainly the role of thermal stratification in street canyon ventilation. In order to fill this gap, this study evaluates how the combined effect of street canyon geometry, wall roughness and differential heating of the building facades influences pollutant dispersion within the canyon and out of it. The study was carried out in a wind tunnel, adopting an idealized urban geometrymade up of square bars placed normal to thewind direction. The boundary conditions inside the canyon were modified by heating its windward and leeward walls, by changing its aspect ratio and by introducing roughness elements at the walls. A passive scalar was injected from a line source at ground level. The flow and concentration fields were measured in a cross-section of the canyon. Characteristic exchange velocities within the canyon and towards the external flow were estimated comparing the experimental data with an analytical model for the cavity wash-out. Results show that the transition from one recirculating cell to two counter-rotating cells inhibits canyon ventilation, with a consequent increase in pollutant concentration at the pedestrian level. This transition occurs as the cavity aspect ratio increases and is facilitated by adding roughness elements at the windward wall. Heating the leeward wall has negligible effects on canyon ventilation. Heating the windward wall accelerates pollutant removals in square cavities, while it contributes to a worsening of air quality in narrow cavities. Finally, the wash-out times of the cavity are discussed in terms of a relative contribution of the mean advectivemotion and its turbulent counterpart

Seasonal changes in vertebrate immune activity: mediation by physiological trade-offs

Animals living in temporally dynamic environments experience variation in resource availability, climate and threat of infection over the course of the year. Thus, to survive and reproduce successfully, these organisms must allocate resources among competing physiological systems in such a way as to maximize fitness in changing environments. Here, we review evidence supporting the hypothesis that physiological trade-offs, particularly those between the reproductive and immune systems, mediate part of the seasonal changes detected in the immune defences of many vertebrates. Abundant recent work has detected significant energetic and nutritional costs of immune defence. Sometimes these physiological costs are sufficiently large to affect fitness (e.g. reproductive output, growth or survival), indicating that selection for appropriate allocation strategies probably occurred in the past. Because hormones often orchestrate allocations among physiological systems, the endocrine mediators of seasonal changes in immune activity are discussed. Many hormones, including melatonin, glucocorticoids and androgens have extensive and consistent effects on the immune system, and they change in systematic fashions over the year. Finally, a modified framework within which to conduct future studies in ecological immunology is proposed, viz. a heightened appreciation of the complex but intelligible nature of the vertebrate immune system. Although other factors besides trade-offs undoubtedly influence seasonal variation in immune defence in animals, a growing literature supports a role for physiological trade-offs and the fitness consequences they sometimes produce

Seminiferous cord formation and germ-cell programming: epigenetic transgenerational actions of endocrine disruptors

The molecular and cellular control of embryonic testis development was investigated through an analysis of the embryonic testis transcriptome to identify potential regulatory factors for male sex determination and testis morphogenesis. One critical factor identified is neurotropin 3 (NT3). At the onset of male sex determination, Sertoli cells initiate differentiation and express NT3 to act as a chemotactic factor for mesonephros cells to migrate and associate with Sertoli-germ cell aggregates to promote cord formation. Promoter analysis suggests that NT3 may be an initial downstream gene to SRY and helps promote testis morphogenesis. Endocrine disruptors were used to potentially interfere with embryonic testis development and further investigate this biological process. The estrogenic pesticide methoxychlor and antiandrogenic fungicide vinclozolin were used. Previous studies have shown that methoxychlor and vinclozolin both interfere with embryonic testis cord formation and cause increased spermatogenic cell apoptosis in the adult testis. Interestingly, transient in vivo exposure to endocrine disruptors at the time of male sex determination caused a transgenerational phenotype (F1-F4) of spermatogenic cell apoptosis and subfertility. This apparent epigenetic mechanism involves altered DNA methylation and permanent re-programming of the male germ-line. A series of genes with altered DNA methylation and imprinting are being identified. Observations reviewed demonstrate that a transient embryonic in utero exposure to an endocrine disruptor influences the embryonic testis transcriptome and through epigenetic effects (e.g., DNA methylation) results in abnormal germ-cell differentiation that subsequently influences adult spermatogenic capacity and male fertility, and that this phenotype is transgenerational through the germ-line. The novel observations of transgenerational epigenetic endocrine disruptor actions on male reproduction critically impact the potential hazards of these compounds as environmental toxins. The literature reviewed provides insight into the molecular and cellular control of embryonic testis development, male sex determination, and the programming of the male germ-line