Atl1 Regulates Choice between Global Genome and Transcription-Coupled Repair of O6-Alkylguanines

Nucleotide excision repair (NER) has long been known to remove DNA lesions induced by chemical carcinogens, and the molecular mechanism has been partially elucidated. Here we demonstrate that in Schizosaccharomyces pombe a DNA recognition protein, alkyltransferase-like 1 (Atl1), can play a pivotal role in selecting a specific NER pathway, depending on the nature of the DNA modification. The relative ease of dissociation of Atl1 from DNA containing small O6-alkylguanines allows accurate completion of global genome repair (GGR), whereas strong Atl1 binding to bulky O6-alkylguanines blocks GGR, stalls the transcription machinery, and diverts the damage to transcription-coupled repair. Our findings redraw the initial stages of the NER process in those organisms that express an alkyltransferase-like gene and raise the question of whether or not O6-alkylguanine lesions that are poor substrates for the alkyltransferase proteins in higher eukaryotes might, by analogy, signal such lesions for repair by NER

African resources vol. 2 Management

SIGLEAvailable from British Library Document Supply Centre- DSC:4126.581(RU-GP--97) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

African resources Vol. 1; appraisal and monitoring

2.00; papers by Allen, T.; Berry, L.; Hulme, M.; Matheson, W.; Olofin, E.; Ringrose, S. and Vujakovic, PAvailable from British Library Document Supply Centre- DSC:4126.581(RU-GP--96) / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo